Detailed information NTG0090158

Bioinformatically predicted

Overview

• Name: pilF
• Type: Machinery gene
• Locus tag: TthTMY_RS00095
• Genome accession: NZ_AP025158
• Coordinates: 18694..21363 (-)
• Length: 889 a.a.
• NCBI ID: WP_096411376.1
• Uniprot ID: -
• Organism: Thermus thermophilus strain TMY
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 13694..26363

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
TthTMY_RS00075 (TthTMY_00120)	-	14540..15172 (-)	633	WP_096411372.1	histidine phosphatase family protein	-
TthTMY_RS00080 (TthTMY_00130)	purM	15169..16170 (-)	1002	WP_096411373.1	phosphoribosylformylglycinamidine cyclo-ligase	-
TthTMY_RS00085 (TthTMY_00140)	gatB	16176..17585 (-)	1410	WP_096411374.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB	-
TthTMY_RS00090 (TthTMY_00150)	pilT	17596..18681 (-)	1086	WP_096411375.1	type IV pilus twitching motility protein PilT	Machinery gene
TthTMY_RS00095 (TthTMY_00160)	pilF	18694..21363 (-)	2670	WP_096411376.1	type IV pilus assembly ATPase PilB	Machinery gene
TthTMY_RS00100 (TthTMY_00170)	-	21360..21845 (-)	486	WP_096411377.1	YqeG family HAD IIIA-type phosphatase	-
TthTMY_RS00105 (TthTMY_00180)	pgeF	21880..22608 (-)	729	WP_096411378.1	peptidoglycan editing factor PgeF	-
TthTMY_RS00110 (TthTMY_00190)	surE	22605..23360 (-)	756	WP_096411379.1	5'/3'-nucleotidase SurE	-
TthTMY_RS00115 (TthTMY_00200)	-	23419..23625 (-)	207	WP_096411380.1	cold-shock protein	-
TthTMY_RS00120 (TthTMY_00210)	-	23787..24884 (+)	1098	WP_096410490.1	IS4 family transposase	-
TthTMY_RS00125 (TthTMY_00220)	-	24965..26254 (+)	1290	WP_096411381.1	ABC transporter substrate-binding protein	-

Sequence

Protein

• Length: 889 a.a.
• Molecular weight: 98149.21 Da
• Isoelectric Point: 5.3343

>NTDB_id=90158 TthTMY_RS00095 WP_096411376.1 18694..21363(-) (pilF) [Thermus thermophilus strain TMY]
MSVLTIGDKRLGAALLDAGLLTDEELQRALERHREVGGSLAEVLVDMGLLSERRIAQTIEDHFGIPLVELPQVEIPPKAK
ALLPAEKAKELKAIPFALDEEAGVVRVAFLNPLDTLSLEEVEDLTGLVVEPYQTTKSAFLYALAKHYPELGLPVPPPPSG
EGQKELKLGELLLQKGWISREALEEALVEQEKTGDLLGRILVRKGLPEEALYRALAEQKGLEFMESTEGIAPDPSAALLL
LRSDALRYGAVPIGFRNGKVEVVLSDPRHKETVAQLLNRPVRFYLTLPQAWEELFRRAYPQKNRLGEVLVQEGKLSREAL
KEALEVQKGLPRAKSLGEILVELGLARPEDVEEALKKQRQGGGRLEDTLVQSGKLRPEALAQAVATQLGYPYVDPEEDPP
DPGASLLLPEDLCRRYGVFPHRLEGDRLVLLMKDPRNILALDDVRLALKRKGLDHEVAPAVATEAAITKLIERFYGKAEL
SEIAKEFAKKQAEEEVASPLELDESAAQKFVKQVIREAYLQDASDIHIEPRQNDVQVRLRIDGALRPYSTLPKGALNAVI
SVVKIMGGLNIAEKRLPQDGRVRYREGAIDVDLRLSTLPTVYGEKAVMRLLKKASDIPEIEELGFAPGVFERFKEVISKP
YGIFLITGPTGSGKSFTTFSILKRIATPDKNTQTIEDPVEYEIPGINQTQVNPQAGLTFARALRAFLRQDPDIIMVGEIR
DSETAKIATEAALTGHLVIATLHTNDAAQAITRLDEMGVEPFNISAALIGVLSQRLVRRVCEHCKVEVKPDPETLRRLGL
SEAEIQGARLYKGMGCERCGGTGYKGRYAIHELLVVDDEIRHAIVAGKSATEIKEIARRKGMKTLREDGLYKALQGITTL
EEVLARTIE

Nucleotide

• Length: 2670 bp

>NTDB_id=90158 TthTMY_RS00095 WP_096411376.1 18694..21363(-) (pilF) [Thermus thermophilus strain TMY]
ATGAGCGTGCTCACCATAGGGGACAAAAGGCTCGGGGCGGCCCTCTTGGACGCCGGGCTCCTCACGGACGAGGAACTGCA
GCGGGCCTTGGAACGGCACCGGGAGGTGGGGGGCTCCTTGGCCGAGGTCCTGGTGGACATGGGCCTCCTCTCCGAGCGGA
GGATCGCCCAGACCATTGAGGACCACTTCGGCATCCCCCTGGTGGAGCTCCCCCAAGTGGAGATCCCCCCCAAGGCCAAG
GCCCTCCTTCCCGCGGAAAAGGCCAAAGAACTCAAGGCCATCCCCTTCGCCCTGGACGAGGAGGCGGGGGTGGTGCGGGT
GGCCTTCCTCAACCCCCTGGACACCCTGAGCCTCGAGGAGGTGGAGGACCTCACGGGGCTCGTGGTGGAGCCATACCAGA
CCACCAAAAGCGCCTTCCTCTACGCCCTGGCCAAGCACTACCCGGAGCTCGGCCTCCCCGTCCCCCCGCCGCCTTCGGGC
GAGGGCCAGAAGGAGCTCAAGCTCGGGGAGCTCCTCCTGCAAAAGGGGTGGATCTCCCGCGAGGCCCTGGAGGAGGCCTT
GGTGGAACAGGAGAAGACGGGGGACCTCCTCGGGCGGATCCTGGTGCGGAAGGGGCTCCCCGAGGAGGCCCTTTACCGGG
CTTTGGCGGAGCAGAAGGGGCTGGAGTTTATGGAAAGCACCGAGGGGATCGCCCCCGACCCTTCCGCCGCCCTCCTCCTC
CTCCGCTCCGATGCCCTGCGCTACGGCGCCGTGCCCATCGGCTTCCGAAACGGGAAGGTGGAGGTGGTCCTCTCCGACCC
CCGGCACAAGGAGACGGTGGCCCAACTCCTCAATCGGCCCGTCCGCTTCTACCTCACCCTCCCCCAGGCCTGGGAGGAGC
TCTTCCGCCGGGCCTACCCGCAGAAGAACCGCCTGGGGGAGGTCCTGGTCCAGGAGGGCAAGCTCTCCCGGGAGGCGCTG
AAAGAAGCCCTGGAGGTGCAGAAAGGCCTGCCTCGGGCCAAGTCTTTGGGGGAGATCCTGGTGGAGCTCGGCCTCGCCCG
CCCCGAGGACGTGGAGGAGGCCCTGAAGAAGCAAAGGCAGGGCGGAGGCCGGCTGGAGGACACCCTGGTCCAGTCGGGGA
AGCTCAGGCCCGAGGCCCTGGCCCAGGCGGTGGCCACCCAGCTGGGCTACCCCTACGTTGACCCCGAGGAGGACCCCCCC
GATCCCGGCGCCTCCCTCCTCCTCCCGGAGGACCTGTGCCGCCGCTATGGGGTCTTCCCCCACCGCCTCGAGGGAGACCG
CCTCGTCCTCCTGATGAAGGACCCGAGGAACATCCTGGCCCTGGACGACGTGCGCCTCGCCCTCAAGAGGAAGGGCCTGG
ACCACGAGGTGGCCCCCGCCGTGGCCACGGAGGCCGCCATCACCAAGCTCATTGAGCGCTTCTACGGCAAGGCCGAGCTC
TCGGAGATCGCCAAGGAATTCGCCAAAAAGCAGGCGGAGGAGGAGGTCGCAAGCCCCCTGGAGCTGGACGAGAGCGCCGC
CCAGAAGTTCGTGAAGCAGGTGATCCGGGAGGCCTACCTCCAGGACGCCTCCGACATCCACATTGAGCCCAGGCAGAACG
ACGTCCAGGTGCGCCTCCGGATTGACGGCGCCCTGCGGCCGTACAGCACCCTACCCAAGGGGGCGCTAAACGCGGTGATC
TCCGTGGTCAAGATCATGGGCGGGCTCAACATCGCCGAGAAGCGCCTCCCCCAGGACGGACGGGTGCGCTACCGGGAGGG
GGCCATAGACGTGGACCTCCGGCTTTCCACCCTGCCCACGGTCTACGGGGAGAAGGCGGTGATGCGCCTGCTCAAGAAGG
CCTCGGACATCCCCGAGATCGAGGAGCTTGGCTTCGCCCCGGGGGTGTTTGAACGCTTTAAGGAGGTGATCTCCAAACCC
TACGGCATCTTCCTCATCACCGGGCCCACAGGGTCAGGCAAGAGCTTCACCACCTTCTCCATCCTGAAGCGCATCGCCAC
CCCCGACAAGAACACCCAGACCATTGAGGACCCCGTGGAGTACGAGATCCCGGGCATCAACCAGACCCAGGTGAACCCGC
AGGCGGGCCTCACCTTCGCCCGGGCGCTAAGGGCCTTCCTCAGGCAGGACCCGGACATCATCATGGTAGGGGAGATCCGG
GACTCCGAGACGGCCAAGATCGCCACCGAGGCCGCCCTCACCGGCCACCTCGTCATCGCCACCCTGCACACCAACGACGC
CGCCCAGGCCATCACCCGCCTGGACGAGATGGGGGTGGAACCCTTTAACATCTCCGCTGCCCTCATCGGCGTCCTCTCCC
AGCGCCTGGTGCGCAGGGTATGCGAGCACTGCAAGGTGGAGGTCAAGCCGGACCCCGAGACCCTAAGGCGCCTTGGGCTT
TCCGAGGCGGAGATCCAAGGGGCCAGGCTCTATAAGGGCATGGGGTGCGAGCGGTGCGGCGGCACCGGCTACAAGGGCCG
CTACGCCATCCACGAGCTTTTGGTGGTGGACGACGAGATCCGCCACGCCATCGTGGCGGGGAAGTCGGCCACGGAGATCA
AGGAGATCGCCCGGAGGAAGGGGATGAAAACCCTGAGGGAGGACGGCCTCTACAAGGCCCTCCAAGGGATCACCACCCTC
GAGGAGGTCCTGGCGCGTACCATTGAGTAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (369-479)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (201-302)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (44-148)

• Type II/IV secretion system protein (T2SSE) (513-779)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilF	Thermus thermophilus HB27	97.525	100	0.975
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	56.067	100	0.561