NTDB

Overview

Name	pilT	Type	Machinery gene
Locus tag	R2B70_RS17215	Genome accession	NZ_CP137630
Coordinates	3740371..3741405 (+)	Length	344 a.a.
NCBI ID	WP_042079327.1	Uniprot ID	-
Organism	Aeromonas sp. XH
Function	mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3735371..3746405

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R2B70_RS17175 (R2B70_17175)	-	3735479..3735853 (+)	375	WP_209793640.1	YacL family protein	-
R2B70_RS17180 (R2B70_17180)	-	3735904..3736194 (+)	291	WP_042079320.1	hypothetical protein	-
R2B70_RS17185 (R2B70_17185)	-	3736388..3737218 (+)	831	WP_318162066.1	CPBP family intramembrane glutamic endopeptidase	-
R2B70_RS17190 (R2B70_17190)	-	3737297..3737716 (-)	420	WP_042079322.1	DUF4426 domain-containing protein	-
R2B70_RS17195 (R2B70_17195)	yggU	3737721..3738029 (-)	309	WP_042079323.1	DUF167 family protein YggU	-
R2B70_RS17200 (R2B70_17200)	-	3738029..3738580 (-)	552	WP_209793643.1	YggT family protein	-
R2B70_RS17205 (R2B70_17205)	proC	3738605..3739429 (-)	825	WP_318162067.1	pyrroline-5-carboxylate reductase	-
R2B70_RS17210 (R2B70_17210)	-	3739630..3740331 (-)	702	WP_042079326.1	YggS family pyridoxal phosphate-dependent enzyme	-
R2B70_RS17215 (R2B70_17215)	pilT	3740371..3741405 (+)	1035	WP_042079327.1	type IVa pilus ATPase TapT	Machinery gene
R2B70_RS17220 (R2B70_17220)	pilU	3741429..3742538 (+)	1110	WP_042079328.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
R2B70_RS17225 (R2B70_17225)	yaaA	3742570..3743343 (+)	774	WP_318162068.1	peroxide stress protein YaaA	-
R2B70_RS17230 (R2B70_17230)	srmB	3743517..3744737 (-)	1221	WP_041210934.1	ATP-dependent RNA helicase SrmB	-
R2B70_RS17235 (R2B70_17235)	-	3744865..3745575 (+)	711	WP_318162069.1	tRNA1(Val) (adenine(37)-N6)-methyltransferase	-

Sequence

Protein

Download Length: 344 a.a. Molecular weight: 38203.98 Da Isoelectric Point: 6.7437

                                >NTDB_id=899534 R2B70_RS17215 WP_042079327.1 3740371..3741405(+) (pilT) [Aeromonas sp. XH]

MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALDHREVHALIYDIMNDHQRKELEENFEVDFSFEVPGMAR

FRVNAFQQARGSGAVFRTIPSTVLTLDDLAAPEIFRKIAEFPRGLVLVTGPTGSGKSTTLAAMVDYINDNFHHHILTIED

PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI

IDVFPGSEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMMGIPAVRNLIREDKVAQLYSVIQTGMTHGMQTMDQSL

KQLVNRGIVAALDAKAKAVDPNTI

Nucleotide

Download Length: 1035 bp

                                >NTDB_id=899534 R2B70_RS17215 WP_042079327.1 3740371..3741405(+) (pilT) [Aeromonas sp. XH]

ATGGATATCACAGAGTTATTGGCATTCAGTGTAAAGCATAAGGCCTCGGATCTACACCTCTCCGCCGGGGTCCCTCCGAT

GATCAGGGTTGACGGGGAGGTTCGCAAGATCAATTTGCCCGCCCTGGATCACCGGGAAGTCCACGCCCTCATCTACGACA

TCATGAACGACCACCAGCGCAAGGAGCTGGAGGAGAACTTCGAGGTGGACTTCTCCTTCGAGGTGCCCGGCATGGCGCGC

TTCCGGGTCAACGCCTTCCAGCAGGCGCGGGGCTCGGGCGCCGTGTTCCGTACCATTCCGAGCACAGTCCTGACCCTGGA

CGATCTGGCGGCACCCGAGATATTTCGCAAGATAGCGGAGTTTCCCCGCGGCCTAGTGCTGGTGACAGGGCCGACCGGTT

CGGGCAAGTCCACCACCCTGGCGGCCATGGTCGACTACATCAACGACAACTTCCACCACCATATCCTCACCATCGAGGAT

CCCATCGAATTCGTGCACGAGAACAAGCGCTGCCTAGTCAACCAGCGGGAGGTGCACCGCGACACCAAGAGCTTCAGCAA

CGCCCTGCGCTCGGCCCTGCGGGAAGACCCGGACATCATACTGGTGGGGGAGATGCGCGACCTCGAGACCATCCGCCTCG

CCATGACGGCGGCCGAGACCGGCCACCTGGTGTTTGGCACCCTGCACACCTCGTCGGCGGCCAAGACCATAGACCGTATC

ATCGACGTCTTCCCCGGCTCGGAGAAGGACATGGTGCGTTCCATGCTGTCCGAATCCCTGCGGGCGGTCATCTCCCAGAC

CCTGCTCAAGCGGATCGGGGGTGGCCGGGTGGCGGCCCACGAGATCATGATGGGCATTCCCGCCGTGCGCAACCTCATCC

GGGAGGACAAGGTGGCCCAGCTCTATTCCGTCATCCAGACCGGGATGACCCACGGCATGCAGACCATGGACCAGAGCCTC

AAGCAGCTGGTCAACCGGGGGATCGTCGCCGCCCTCGACGCCAAGGCCAAGGCGGTCGACCCCAACACCATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilT	Acinetobacter baylyi ADP1	77.843	99.709	0.776
pilT	Pseudomonas stutzeri DSM 10701	78.006	99.128	0.773
pilT	Acinetobacter baumannii D1279779	76.163	100	0.762
pilT	Acinetobacter baumannii strain A118	76.163	100	0.762
pilT	Acinetobacter nosocomialis M2	76.163	100	0.762
pilT	Pseudomonas aeruginosa PAK	76.54	99.128	0.759
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	75.668	97.965	0.741
pilT	Vibrio cholerae strain A1552	75.668	97.965	0.741
pilT	Legionella pneumophila strain Lp02	72.059	98.837	0.712
pilT	Legionella pneumophila strain ERS1305867	72.059	98.837	0.712
pilT	Neisseria meningitidis 8013	69.027	98.547	0.68
pilT	Neisseria gonorrhoeae MS11	68.732	98.547	0.677
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	52.339	99.419	0.52
pilU	Vibrio cholerae strain A1552	42.899	100	0.43
pilU	Pseudomonas stutzeri DSM 10701	41.159	100	0.413
pilU	Acinetobacter baylyi ADP1	40.462	100	0.407

Detailed information NTG0899534