Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   R2B70_RS17220 Genome accession   NZ_CP137630
Coordinates   3741429..3742538 (+) Length   369 a.a.
NCBI ID   WP_042079328.1    Uniprot ID   -
Organism   Aeromonas sp. XH     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3736429..3747538
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R2B70_RS17190 (R2B70_17190) - 3737297..3737716 (-) 420 WP_042079322.1 DUF4426 domain-containing protein -
  R2B70_RS17195 (R2B70_17195) yggU 3737721..3738029 (-) 309 WP_042079323.1 DUF167 family protein YggU -
  R2B70_RS17200 (R2B70_17200) - 3738029..3738580 (-) 552 WP_209793643.1 YggT family protein -
  R2B70_RS17205 (R2B70_17205) proC 3738605..3739429 (-) 825 WP_318162067.1 pyrroline-5-carboxylate reductase -
  R2B70_RS17210 (R2B70_17210) - 3739630..3740331 (-) 702 WP_042079326.1 YggS family pyridoxal phosphate-dependent enzyme -
  R2B70_RS17215 (R2B70_17215) pilT 3740371..3741405 (+) 1035 WP_042079327.1 type IVa pilus ATPase TapT Machinery gene
  R2B70_RS17220 (R2B70_17220) pilU 3741429..3742538 (+) 1110 WP_042079328.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  R2B70_RS17225 (R2B70_17225) yaaA 3742570..3743343 (+) 774 WP_318162068.1 peroxide stress protein YaaA -
  R2B70_RS17230 (R2B70_17230) srmB 3743517..3744737 (-) 1221 WP_041210934.1 ATP-dependent RNA helicase SrmB -
  R2B70_RS17235 (R2B70_17235) - 3744865..3745575 (+) 711 WP_318162069.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  R2B70_RS17240 (R2B70_17240) brnQ 3745811..3747073 (+) 1263 WP_042079405.1 branched-chain amino acid transport system II carrier protein -
  R2B70_RS17245 (R2B70_17245) - 3747165..3747392 (-) 228 WP_042079332.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41035.03 Da        Isoelectric Point: 6.0456

>NTDB_id=899535 R2B70_RS17220 WP_042079328.1 3741429..3742538(+) (pilU) [Aeromonas sp. XH]
MDMDVLLAALVEQKGSDLFITVDAPPTLKVNGRLVPLGETPLDRQTALALVKSSLDAEHFERYQHTREANYAIHREGLGR
FRVSAFWQQDLPGMVLRRIETRIPTFDELVLPPILQDVAMAKRGLVLFVGATGAGKSTTQAAMLGYRNQHGDGHILTVED
PVEFVHQHGRCLVTQREVGIDTGSFDVALRNSLRQAPDVILIGEIRNQETMEFAIQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSIDGSRRFAAFEILLNTPLITDIVRKGEMHRLKEVMTKSTELGMQTFDQA
LFTLFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=899535 R2B70_RS17220 WP_042079328.1 3741429..3742538(+) (pilU) [Aeromonas sp. XH]
ATGGACATGGATGTTCTGCTCGCAGCACTGGTTGAACAAAAGGGCTCGGACCTCTTCATCACGGTCGATGCCCCCCCCAC
TCTCAAGGTGAATGGGCGCCTGGTGCCGCTCGGAGAAACCCCGCTGGACAGACAGACCGCGCTGGCCCTGGTCAAGAGCA
GCCTGGATGCCGAGCATTTCGAACGCTACCAGCACACCCGGGAGGCCAACTACGCCATCCATCGCGAGGGGCTTGGGCGC
TTTCGGGTCAGCGCCTTCTGGCAACAGGACCTGCCGGGCATGGTGCTGCGCCGCATCGAGACCCGCATTCCCACCTTCGA
CGAGCTGGTGCTGCCCCCGATATTGCAGGACGTCGCCATGGCCAAGCGCGGCCTGGTGCTGTTCGTCGGGGCCACGGGCG
CCGGCAAATCCACCACCCAGGCGGCCATGCTCGGCTATCGCAACCAGCATGGCGACGGCCACATCCTGACGGTGGAAGAC
CCGGTGGAGTTCGTCCACCAGCACGGTCGCTGCCTGGTGACCCAGCGGGAGGTCGGCATCGACACCGGCTCCTTCGACGT
GGCGCTCAGAAACTCCCTGCGCCAGGCACCGGACGTCATCTTGATCGGCGAGATCCGCAACCAGGAGACCATGGAGTTCG
CCATCCAGTTTGCCGAGACGGGCCACCTCTGCCTCGCCACCCTGCACGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTCCACCTGGTTCCCCAGGAGAAGCACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGCGCCATCGTCGCACAACA
ACTGCTGCCGAGCATCGACGGGAGTCGCCGCTTCGCCGCCTTCGAGATCCTGCTCAACACCCCCCTCATCACCGACATCG
TCCGCAAGGGGGAGATGCATCGGCTCAAGGAGGTGATGACCAAGTCCACCGAGCTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCACCCTGTTCTGCGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCCGCCAACGACCTGCGACT
GCTGATCAAGCTGTCGGGGCGCGAGCAGCTGGGTGCAGGTACTCTCGACAACGTCACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(119-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.054

99.729

0.599
  pilU Pseudomonas stutzeri DSM 10701

59.829

95.122

0.569
  pilU Acinetobacter baylyi ADP1

56.657

95.664

0.542
  pilT Legionella pneumophila strain Lp02

40.816

92.954

0.379
  pilT Legionella pneumophila strain ERS1305867

40.816

92.954

0.379
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.708

91.87

0.374
  pilT Acinetobacter baylyi ADP1

39.589

92.412

0.366