Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   R5H13_RS03525 Genome accession   NZ_CP137578
Coordinates   840866..841912 (+) Length   348 a.a.
NCBI ID   WP_130126814.1    Uniprot ID   -
Organism   Pseudoalteromonas maricaloris strain DSM 15914     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 835866..846912
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R5H13_RS03495 (R5H13_03495) hemW 836594..837730 (-) 1137 WP_193522437.1 radical SAM family heme chaperone HemW -
  R5H13_RS03500 (R5H13_03500) - 837727..838320 (-) 594 WP_193522438.1 XTP/dITP diphosphatase -
  R5H13_RS03505 (R5H13_03505) - 838329..838763 (-) 435 WP_130126816.1 DUF4426 domain-containing protein -
  R5H13_RS03510 (R5H13_03510) - 838774..839307 (-) 534 WP_010379021.1 YggT family protein -
  R5H13_RS03515 (R5H13_03515) proC 839317..840138 (-) 822 WP_039494449.1 pyrroline-5-carboxylate reductase -
  R5H13_RS03520 (R5H13_03520) - 840152..840835 (-) 684 WP_130126815.1 YggS family pyridoxal phosphate-dependent enzyme -
  R5H13_RS03525 (R5H13_03525) pilT 840866..841912 (+) 1047 WP_130126814.1 type IV pilus twitching motility protein PilT Machinery gene
  R5H13_RS03530 (R5H13_03530) pilU 841927..843048 (+) 1122 WP_010607713.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  R5H13_RS03535 (R5H13_03535) - 843026..843910 (+) 885 WP_130126813.1 hypothetical protein -
  R5H13_RS03540 (R5H13_03540) ruvX 844227..844676 (-) 450 WP_039494452.1 Holliday junction resolvase RuvX -
  R5H13_RS03545 (R5H13_03545) - 844719..845276 (-) 558 WP_010379034.1 YqgE/AlgH family protein -
  R5H13_RS03550 (R5H13_03550) gshB 845296..846255 (-) 960 WP_010379037.1 glutathione synthase -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38649.92 Da        Isoelectric Point: 6.3154

>NTDB_id=899187 R5H13_RS03525 WP_130126814.1 840866..841912(+) (pilT) [Pseudoalteromonas maricaloris strain DSM 15914]
MDITELLAFSVQHKASDLHLSSGVSPMIRVDGDVRRVNIPPLEVKDVSSLIYDIMNDNQRRDYEQNLEVDFSFEVPNLAR
FRVNAFNSNRGPAAVFRTIPNQVLSLDDLGAPDIFTKISDNPRGLVLVTGPTGSGKSTTLAAMVDYINNNKHHHILTIED
PIEFVHDNKQSLINQREVHRDTHSFNAALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTTSAPKTIDRI
VDVFPGEEKDMVRSMLSESLQAVISQTLVKKVGGGRIAAHEIMLGIPAIRNLIREDKIAQMYSAIQTGAMHGMQTMDQCL
TNLLNRGLITQQDARAKAHDKNQFNSGY

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=899187 R5H13_RS03525 WP_130126814.1 840866..841912(+) (pilT) [Pseudoalteromonas maricaloris strain DSM 15914]
ATGGATATTACAGAATTATTGGCTTTTAGTGTTCAACACAAAGCATCTGACTTACACCTTTCTTCAGGTGTTTCCCCTAT
GATCCGTGTTGACGGGGACGTACGCCGGGTGAATATTCCGCCGTTAGAAGTAAAAGACGTCAGCAGCCTCATCTACGATA
TTATGAATGATAATCAACGTCGTGACTATGAGCAAAACCTTGAGGTAGATTTCTCGTTCGAAGTACCAAATCTGGCGCGT
TTTCGTGTTAACGCCTTTAATTCAAATCGTGGTCCTGCTGCGGTGTTCCGTACCATTCCAAATCAAGTGCTGAGCTTAGA
TGATTTGGGGGCACCAGACATTTTTACCAAAATTTCCGACAACCCACGTGGCTTAGTATTGGTAACCGGCCCGACAGGCT
CTGGTAAATCAACCACGCTGGCTGCGATGGTGGATTATATTAATAACAACAAACATCACCATATTCTAACCATCGAAGAC
CCGATTGAATTTGTTCACGACAACAAGCAAAGCCTTATTAACCAACGTGAAGTACATAGAGATACGCACAGCTTTAACGC
GGCGCTGAGAAGTGCACTCCGTGAAGACCCAGACGTTATTTTAGTTGGTGAATTACGTGATCTTGAGACCATTCGTTTAG
CGCTAACTGCCGCGGAAACAGGTCACTTAGTATTTGGTACTTTGCATACTACCTCAGCACCTAAAACCATCGACCGTATT
GTGGATGTGTTCCCAGGTGAAGAAAAAGACATGGTGCGTTCTATGCTGTCAGAGTCGCTACAAGCGGTGATTTCACAAAC
ACTGGTGAAAAAAGTTGGCGGCGGGCGCATAGCAGCGCATGAAATCATGCTCGGGATCCCTGCTATTCGTAACCTTATTC
GTGAAGACAAGATTGCACAGATGTATTCGGCAATTCAAACCGGTGCAATGCACGGTATGCAAACCATGGATCAATGCCTT
ACAAATTTATTAAACCGTGGTTTGATCACACAGCAAGATGCGCGTGCAAAAGCGCATGACAAAAATCAGTTTAATAGCGG
CTATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.035

98.851

0.761
  pilT Acinetobacter baumannii D1279779

77.035

98.851

0.761
  pilT Acinetobacter baumannii strain A118

77.035

98.851

0.761
  pilT Acinetobacter baylyi ADP1

76.744

98.851

0.759
  pilT Pseudomonas stutzeri DSM 10701

75.872

98.851

0.75
  pilT Pseudomonas aeruginosa PAK

75.581

98.851

0.747
  pilT Legionella pneumophila strain Lp02

71.221

98.851

0.704
  pilT Legionella pneumophila strain ERS1305867

71.221

98.851

0.704
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.217

96.839

0.69
  pilT Vibrio cholerae strain A1552

71.217

96.839

0.69
  pilT Neisseria meningitidis 8013

69.208

97.989

0.678
  pilT Neisseria gonorrhoeae MS11

68.915

97.989

0.675
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.754

99.138

0.523
  pilU Vibrio cholerae strain A1552

40.525

98.563

0.399
  pilU Pseudomonas stutzeri DSM 10701

39.942

98.563

0.394
  pilU Acinetobacter baylyi ADP1

38.953

98.851

0.385