Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   R5H13_RS03530 Genome accession   NZ_CP137578
Coordinates   841927..843048 (+) Length   373 a.a.
NCBI ID   WP_010607713.1    Uniprot ID   A0A2K9JUM3
Organism   Pseudoalteromonas maricaloris strain DSM 15914     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 836927..848048
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R5H13_RS03500 (R5H13_03500) - 837727..838320 (-) 594 WP_193522438.1 XTP/dITP diphosphatase -
  R5H13_RS03505 (R5H13_03505) - 838329..838763 (-) 435 WP_130126816.1 DUF4426 domain-containing protein -
  R5H13_RS03510 (R5H13_03510) - 838774..839307 (-) 534 WP_010379021.1 YggT family protein -
  R5H13_RS03515 (R5H13_03515) proC 839317..840138 (-) 822 WP_039494449.1 pyrroline-5-carboxylate reductase -
  R5H13_RS03520 (R5H13_03520) - 840152..840835 (-) 684 WP_130126815.1 YggS family pyridoxal phosphate-dependent enzyme -
  R5H13_RS03525 (R5H13_03525) pilT 840866..841912 (+) 1047 WP_130126814.1 type IV pilus twitching motility protein PilT Machinery gene
  R5H13_RS03530 (R5H13_03530) pilU 841927..843048 (+) 1122 WP_010607713.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  R5H13_RS03535 (R5H13_03535) - 843026..843910 (+) 885 WP_130126813.1 hypothetical protein -
  R5H13_RS03540 (R5H13_03540) ruvX 844227..844676 (-) 450 WP_039494452.1 Holliday junction resolvase RuvX -
  R5H13_RS03545 (R5H13_03545) - 844719..845276 (-) 558 WP_010379034.1 YqgE/AlgH family protein -
  R5H13_RS03550 (R5H13_03550) gshB 845296..846255 (-) 960 WP_010379037.1 glutathione synthase -
  R5H13_RS03555 (R5H13_03555) rsmE 846313..847047 (-) 735 WP_039494453.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  R5H13_RS03560 (R5H13_03560) - 847110..847607 (-) 498 WP_039494456.1 hypothetical protein -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41134.98 Da        Isoelectric Point: 6.0556

>NTDB_id=899188 R5H13_RS03530 WP_010607713.1 841927..843048(+) (pilU) [Pseudoalteromonas maricaloris strain DSM 15914]
MNIEPFLQTMANQQASDLFVSAGLAVSAKIDGELRALSEESLDAEQSLNLVTSIMSDKQKQEFFNTKECNFAIANDIGRF
RVSAFWQRDCAGMVIRRIVTAIPDVNELGLPSVLTDVIMSKRGLVLFVGGTGTGKSTSLAALLGYRNRNQRGHILTIEDP
IEFVHQHQKSIITQREVGIDTDSFESALKSSLRQAPDVILIGEIRSQETMEYALSFAETGHLCVATLHANNANQAIDRIM
HLVPKEKHDKLKYDLALNLRAIVAQQLVPSSKGEGREAAIEVLLNSPLVAELIKKGDIGSIKEAMAKSKDMGMQTFDQAL
FELYKQQRINYADALHHADSPNDLRLMIKLQNNEQKGAGFLQGVTVDGLDSKN

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=899188 R5H13_RS03530 WP_010607713.1 841927..843048(+) (pilU) [Pseudoalteromonas maricaloris strain DSM 15914]
ATGAATATAGAACCATTTTTGCAAACGATGGCCAATCAGCAAGCATCCGACTTGTTCGTTTCGGCAGGGCTTGCGGTGAG
CGCCAAAATCGATGGCGAACTTAGAGCGTTAAGTGAAGAAAGCTTAGATGCCGAGCAGTCACTGAATCTTGTTACCTCGA
TTATGAGCGACAAGCAAAAGCAAGAATTCTTTAACACTAAAGAGTGTAACTTTGCGATTGCCAATGATATCGGGCGTTTT
CGTGTTTCTGCATTTTGGCAACGGGATTGTGCGGGTATGGTTATTCGCCGTATCGTTACCGCGATTCCCGATGTCAATGA
GCTAGGTTTGCCGTCGGTACTGACCGATGTGATCATGTCCAAACGTGGCTTGGTGTTATTTGTAGGTGGTACGGGCACGG
GTAAGTCAACCTCGCTTGCTGCACTGCTCGGTTATCGCAATCGTAACCAACGTGGCCATATTTTGACCATTGAAGATCCC
ATCGAATTTGTGCATCAACACCAAAAAAGCATCATTACTCAGCGTGAAGTGGGCATTGATACTGACAGTTTTGAATCTGC
CTTAAAAAGTTCATTACGCCAAGCGCCAGATGTTATTTTAATTGGTGAAATTCGCTCGCAAGAAACCATGGAGTATGCAC
TGAGCTTTGCTGAAACGGGGCATTTATGCGTTGCAACCTTACACGCCAATAATGCTAACCAAGCCATCGACCGTATCATG
CACTTAGTCCCCAAAGAAAAGCACGATAAACTGAAGTACGATTTGGCGCTGAACTTGCGCGCTATTGTGGCGCAACAACT
AGTGCCTTCTTCAAAAGGTGAAGGTCGAGAAGCAGCCATTGAAGTACTACTGAATTCCCCACTCGTTGCCGAGCTTATTA
AAAAAGGCGATATTGGCTCGATTAAAGAGGCAATGGCAAAATCGAAAGACATGGGCATGCAAACCTTTGATCAAGCGTTG
TTTGAGCTTTATAAGCAGCAGCGGATCAACTATGCCGACGCACTACATCATGCTGACTCGCCAAACGATCTGCGCTTGAT
GATCAAGCTGCAAAATAATGAACAAAAAGGTGCGGGCTTCCTACAAGGGGTTACGGTAGATGGGTTGGATTCGAAGAACT
AA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2K9JUM3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

56.764

100

0.574
  pilU Pseudomonas stutzeri DSM 10701

60.399

94.102

0.568
  pilU Vibrio cholerae strain A1552

56.131

98.391

0.552
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.5

96.515

0.41
  pilT Acinetobacter baylyi ADP1

40.936

91.689

0.375
  pilT Legionella pneumophila strain ERS1305867

41.742

89.276

0.373
  pilT Legionella pneumophila strain Lp02

41.742

89.276

0.373
  pilT Pseudomonas aeruginosa PAK

40.29

92.493

0.373
  pilT Acinetobacter nosocomialis M2

40.351

91.689

0.37
  pilT Acinetobacter baumannii D1279779

40.351

91.689

0.37
  pilT Acinetobacter baumannii strain A118

40.351

91.689

0.37
  pilT Pseudomonas stutzeri DSM 10701

39.42

92.493

0.365