Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   R5R33_RS08630 Genome accession   NZ_CP137555
Coordinates   2000930..2001964 (+) Length   344 a.a.
NCBI ID   WP_318955615.1    Uniprot ID   A0AAU0N4L5
Organism   Microbulbifer pacificus strain SPO729     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1995930..2006964
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R5R33_RS08610 (R5R33_08610) - 1996492..1998468 (-) 1977 WP_318955611.1 dynamin family protein -
  R5R33_RS08615 (R5R33_08615) - 1998635..1999240 (-) 606 WP_318955612.1 YggT family protein -
  R5R33_RS08620 (R5R33_08620) proC 1999252..2000079 (-) 828 WP_318955613.1 pyrroline-5-carboxylate reductase -
  R5R33_RS08625 (R5R33_08625) - 2000126..2000830 (-) 705 WP_318955614.1 YggS family pyridoxal phosphate-dependent enzyme -
  R5R33_RS08630 (R5R33_08630) pilT 2000930..2001964 (+) 1035 WP_318955615.1 type IV pilus twitching motility protein PilT Machinery gene
  R5R33_RS08635 (R5R33_08635) pilU 2002247..2003398 (+) 1152 WP_318955616.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  R5R33_RS08640 (R5R33_08640) - 2003555..2004172 (+) 618 WP_318955617.1 LysE family translocator -
  R5R33_RS08645 (R5R33_08645) - 2004272..2005906 (+) 1635 WP_318955618.1 amidohydrolase -
  R5R33_RS08650 (R5R33_08650) - 2005921..2006679 (-) 759 WP_404810391.1 LytR/AlgR family response regulator transcription factor -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38440.11 Da        Isoelectric Point: 6.5788

>NTDB_id=899042 R5R33_RS08630 WP_318955615.1 2000930..2001964(+) (pilT) [Microbulbifer pacificus strain SPO729]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDVRRINLPPMEHKQVHGLIYEIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNHNRGAGAVFRTIPSKVLTMEDLGMGQVFKQISDTPRGLVLVTGPTGSGKSTTLAAMIDYINDNKYEHILTIED
PIEFVHESKKCLVNQREVHRDTHGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAQEKAMVRSMLSESLQAVISQTLLKRNGGGRIAAHEIMRGTPAIRNLIREDKIAQMYSAIQTGASVGMQTMDQCL
QDLVEKRIISREVAREKAKMPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=899042 R5R33_RS08630 WP_318955615.1 2000930..2001964(+) (pilT) [Microbulbifer pacificus strain SPO729]
ATGGACATTACAGAACTCCTCGCCTTCAGCGCCAAGCAGAACGCTTCCGACTTGCACCTCTCTGCCGGCCTGCCCCCCAT
GATCCGCGTCGACGGCGACGTCCGCCGCATCAACCTGCCGCCCATGGAGCACAAGCAGGTGCACGGCCTGATCTACGAGA
TCATGAACGACAAGCAGCGCAAGGATTACGAGGAGTTCCTGGAAACCGACTTCTCCTTCGAAGTACCCGGTGTCGCCCGC
TTCCGTGTGAATGCGTTCAACCACAACCGCGGCGCCGGCGCCGTGTTCCGGACCATTCCCTCCAAGGTACTCACCATGGA
AGATCTGGGGATGGGCCAGGTGTTCAAGCAGATCTCCGACACCCCCCGCGGCCTGGTACTGGTCACCGGCCCCACCGGTT
CCGGTAAATCCACCACCCTGGCGGCGATGATCGACTACATTAACGACAACAAGTACGAACACATCCTCACCATCGAGGAC
CCGATCGAATTCGTACACGAGTCGAAGAAGTGCCTGGTGAACCAGCGCGAAGTCCACCGCGACACCCACGGCTTCTCCGA
AGCCCTGCGCTCCGCACTGCGTGAAGACCCGGACATCATCCTCGTGGGTGAGATGCGCGACCTCGAGACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGCCACCTGGTATTCGGCACCCTGCACACCACCTCCGCCGCCAAGACCATCGACCGTGTG
GTGGACGTGTTTCCGGCGCAGGAAAAGGCCATGGTGCGCTCCATGCTGTCGGAATCACTGCAGGCGGTGATCTCCCAGAC
CCTGCTGAAGCGCAACGGCGGCGGCCGTATTGCCGCCCACGAAATCATGCGCGGCACCCCGGCGATCCGTAACCTGATCC
GCGAAGACAAGATCGCGCAGATGTACTCCGCCATCCAGACCGGCGCCAGCGTCGGCATGCAGACCATGGACCAGTGCCTG
CAGGACCTGGTGGAAAAACGCATCATCAGCCGCGAAGTCGCGCGCGAAAAAGCGAAGATGCCGGAGAATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

89.535

100

0.895
  pilT Pseudomonas stutzeri DSM 10701

88.081

100

0.881
  pilT Acinetobacter nosocomialis M2

83.43

100

0.834
  pilT Acinetobacter baumannii D1279779

83.43

100

0.834
  pilT Acinetobacter baumannii strain A118

83.43

100

0.834
  pilT Acinetobacter baylyi ADP1

79.942

100

0.799
  pilT Legionella pneumophila strain Lp02

75.444

98.256

0.741
  pilT Legionella pneumophila strain ERS1305867

75.444

98.256

0.741
  pilT Neisseria meningitidis 8013

68.116

100

0.683
  pilT Neisseria gonorrhoeae MS11

67.536

100

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.941

98.837

0.672
  pilT Vibrio cholerae strain A1552

67.941

98.837

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.52
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Vibrio cholerae strain A1552

40.118

98.547

0.395
  pilU Acinetobacter baylyi ADP1

39.017

100

0.392