Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   R5R33_RS08635 Genome accession   NZ_CP137555
Coordinates   2002247..2003398 (+) Length   383 a.a.
NCBI ID   WP_318955616.1    Uniprot ID   A0AAU0N571
Organism   Microbulbifer pacificus strain SPO729     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1997247..2008398
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R5R33_RS08615 (R5R33_08615) - 1998635..1999240 (-) 606 WP_318955612.1 YggT family protein -
  R5R33_RS08620 (R5R33_08620) proC 1999252..2000079 (-) 828 WP_318955613.1 pyrroline-5-carboxylate reductase -
  R5R33_RS08625 (R5R33_08625) - 2000126..2000830 (-) 705 WP_318955614.1 YggS family pyridoxal phosphate-dependent enzyme -
  R5R33_RS08630 (R5R33_08630) pilT 2000930..2001964 (+) 1035 WP_318955615.1 type IV pilus twitching motility protein PilT Machinery gene
  R5R33_RS08635 (R5R33_08635) pilU 2002247..2003398 (+) 1152 WP_318955616.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  R5R33_RS08640 (R5R33_08640) - 2003555..2004172 (+) 618 WP_318955617.1 LysE family translocator -
  R5R33_RS08645 (R5R33_08645) - 2004272..2005906 (+) 1635 WP_318955618.1 amidohydrolase -
  R5R33_RS08650 (R5R33_08650) - 2005921..2006679 (-) 759 WP_404810391.1 LytR/AlgR family response regulator transcription factor -
  R5R33_RS08655 (R5R33_08655) - 2006676..2007920 (-) 1245 WP_318955619.1 histidine kinase -

Sequence

Protein


Download         Length: 383 a.a.        Molecular weight: 42627.93 Da        Isoelectric Point: 6.4440

>NTDB_id=899043 R5R33_RS08635 WP_318955616.1 2002247..2003398(+) (pilU) [Microbulbifer pacificus strain SPO729]
MEIERLLQLVTEKGASDLFITAGVPASIKLHGKVVPVSTTALTPEKARELVIGIMNERQKKEFIESKELNFAISVRNVGR
FRVSAFFQRNLVGMVLRRIETKIPTIDGLGLPEQLKELAMTKRGLIIFVGATGTGKSTSLASMIGHRNEHSKGHIISIED
PIEFIHQHRGCIVTQREVGLDTDSFETALKNTLRQAPDVILIGEVRSRETMDHAIAFAETGHLCLCTLHANNANQALDRI
IHFFPADRHQQLWMDLSLNLRAMVAQQLVPTPDGDGRRACLEIMINTPLMADLIRKGEVHKMKELMKRSTEQGMQTFDQA
LYELYDRGEITYEDALSHADSANDLRLMIKLKSESDAEYLNSAADELSLASDSDGSGGGPRLY

Nucleotide


Download         Length: 1152 bp        

>NTDB_id=899043 R5R33_RS08635 WP_318955616.1 2002247..2003398(+) (pilU) [Microbulbifer pacificus strain SPO729]
ATGGAAATCGAACGACTCCTACAACTGGTGACTGAAAAAGGCGCATCCGACCTGTTCATCACCGCCGGTGTGCCCGCCTC
CATCAAGCTGCACGGCAAAGTCGTGCCGGTCAGCACCACTGCGCTCACGCCGGAAAAAGCCCGCGAACTCGTTATCGGCA
TCATGAACGAGCGGCAGAAAAAAGAGTTCATCGAAAGCAAGGAACTCAACTTTGCCATCTCCGTGCGCAATGTCGGCCGC
TTCCGGGTCTCCGCCTTCTTCCAGCGCAACCTCGTCGGCATGGTGCTGCGCCGGATCGAAACCAAGATCCCCACCATCGA
CGGTCTCGGCCTGCCCGAGCAGCTCAAAGAGCTGGCCATGACCAAGCGCGGCTTGATCATCTTTGTGGGCGCCACCGGTA
CCGGTAAGTCCACCTCACTCGCCTCCATGATCGGCCACCGCAACGAGCACTCCAAGGGTCACATCATCTCCATCGAAGAC
CCCATCGAATTTATCCACCAGCACCGCGGCTGCATCGTCACCCAGCGCGAGGTGGGCCTGGATACCGACTCCTTCGAGAC
CGCGCTGAAGAACACCCTGCGCCAAGCACCCGACGTCATCCTCATTGGTGAGGTGCGCTCGCGCGAGACCATGGACCACG
CCATCGCCTTCGCGGAAACCGGCCACCTGTGCCTGTGCACCCTGCACGCCAACAACGCCAACCAGGCCCTCGACCGGATC
ATCCACTTCTTCCCCGCTGACCGTCATCAGCAACTGTGGATGGACCTGTCACTGAACCTCCGCGCCATGGTTGCCCAGCA
GCTGGTACCCACACCGGATGGTGACGGCCGCCGTGCCTGTCTCGAGATCATGATCAACACCCCGCTGATGGCCGACCTGA
TCCGCAAGGGCGAAGTACACAAAATGAAGGAGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCC
CTGTACGAACTCTACGACCGCGGCGAAATTACCTACGAAGACGCGCTGTCGCACGCTGACTCCGCCAACGACCTGCGCCT
GATGATCAAGCTCAAGTCCGAGTCAGACGCCGAGTACCTGAACAGCGCCGCCGACGAACTCAGTCTTGCCAGCGACTCCG
ACGGCAGCGGCGGCGGCCCGCGCCTTTACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(49-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

75.066

98.433

0.739
  pilU Acinetobacter baylyi ADP1

67.507

93.211

0.629
  pilU Vibrio cholerae strain A1552

56.18

92.95

0.522
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.298

100

0.423
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.136

87.99

0.371
  pilT Vibrio cholerae strain A1552

42.136

87.99

0.371
  pilT Acinetobacter nosocomialis M2

41.298

88.512

0.366
  pilT Acinetobacter baumannii D1279779

41.298

88.512

0.366
  pilT Acinetobacter baumannii strain A118

41.298

88.512

0.366
  pilT Legionella pneumophila strain Lp02

41.791

87.467

0.366
  pilT Legionella pneumophila strain ERS1305867

41.791

87.467

0.366
  pilT Pseudomonas aeruginosa PAK

41.003

88.512

0.363