Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   R5M92_RS13005 Genome accession   NZ_CP137552
Coordinates   2839672..2840706 (-) Length   344 a.a.
NCBI ID   WP_346796380.1    Uniprot ID   -
Organism   Halomonas kashgarensis strain zp-37     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2834672..2845706
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R5M92_RS12990 (R5M92_12990) tyrS 2836021..2837220 (-) 1200 WP_346796376.1 tyrosine--tRNA ligase -
  R5M92_RS12995 (R5M92_12995) - 2837338..2838474 (+) 1137 WP_346796378.1 anhydro-N-acetylmuramic acid kinase -
  R5M92_RS13000 (R5M92_13000) pilU 2838475..2839635 (-) 1161 WP_346796379.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  R5M92_RS13005 (R5M92_13005) pilT 2839672..2840706 (-) 1035 WP_346796380.1 type IV pilus twitching motility protein PilT Machinery gene
  R5M92_RS13010 (R5M92_13010) - 2840741..2841454 (+) 714 WP_346796381.1 YggS family pyridoxal phosphate-dependent enzyme -
  R5M92_RS13015 (R5M92_13015) proC 2841509..2842333 (+) 825 WP_346796382.1 pyrroline-5-carboxylate reductase -
  R5M92_RS13020 (R5M92_13020) - 2842363..2842956 (+) 594 WP_346796383.1 YggT family protein -
  R5M92_RS13025 (R5M92_13025) metX 2842988..2844169 (+) 1182 WP_346796384.1 homoserine O-succinyltransferase MetX -
  R5M92_RS13030 (R5M92_13030) metW 2844169..2844765 (+) 597 WP_346799367.1 methionine biosynthesis protein MetW -
  R5M92_RS13035 (R5M92_13035) - 2844788..2845243 (+) 456 WP_346796385.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37786.21 Da        Isoelectric Point: 6.2449

>NTDB_id=899023 R5M92_RS13005 WP_346796380.1 2839672..2840706(-) (pilT) [Halomonas kashgarensis strain zp-37]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDIRRLNVPAMDNSEVRKLIYDIMGDRQRRDYEEHMETDFSFEVPGVSR
FRVNAFNQARGAGAVFRTIPSQVSSMADLGLGEVFERLAMLPRGLVLVTGPTGSGKSTTLAAMIDYINDKRFEHILTIED
PVEFVHTSKRCLINQREVHRDTHGFAQALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPGEEKAMVRSMLSESLQAVISQSLLKRQGGGRVAAHEILVATAAVRNLIREDKVAQIYSAIQTGGNLGMQTLNSSL
ERLVKEGVVSVEEAQARAKGDLAL

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=899023 R5M92_RS13005 WP_346796380.1 2839672..2840706(-) (pilT) [Halomonas kashgarensis strain zp-37]
ATGGATATTACCGAACTGCTGGCGTTTTCGGCAAAACAGAATGCGTCGGACCTGCACCTGTCGGCGGGCCTGCCGCCGAT
GATACGCGTTGACGGCGATATTCGCCGACTCAATGTTCCCGCCATGGACAATAGCGAGGTGCGCAAGCTGATCTACGACA
TCATGGGGGATCGCCAGCGGCGTGACTATGAAGAGCACATGGAGACGGACTTTTCCTTCGAAGTGCCGGGGGTGTCGCGG
TTTCGCGTCAATGCCTTCAACCAGGCGCGTGGCGCTGGCGCGGTATTTCGCACCATCCCCAGTCAGGTCTCGAGCATGGC
GGATCTCGGCCTGGGGGAGGTGTTCGAGCGACTGGCGATGTTGCCCCGTGGGCTGGTACTGGTGACGGGTCCAACCGGTT
CGGGTAAAAGCACTACCCTGGCGGCGATGATCGATTACATAAACGACAAGCGCTTCGAGCATATCCTCACCATCGAGGAC
CCGGTCGAGTTCGTGCATACCAGCAAACGTTGCCTGATCAACCAGCGTGAAGTGCATCGCGATACCCATGGTTTCGCCCA
GGCGCTGCGCAGCGCGCTGCGTGAAGATCCGGATGTCATCCTGGTGGGTGAGTTGCGCGACCTGGAAACCATTCGTCTGG
CGCTTACCGCAGCGGAAACCGGCCACCTGGTCTTCGGTACACTGCACACCACCTCGGCGGCCAAGACCATCGACCGCATC
ATCGATGTCTTCCCCGGCGAGGAGAAGGCCATGGTGCGTTCGATGCTGTCGGAGTCGCTGCAGGCGGTAATATCGCAATC
GTTGCTCAAGCGTCAGGGCGGCGGGCGCGTGGCGGCCCATGAAATCCTTGTCGCCACAGCGGCGGTGCGTAACTTGATCC
GCGAAGACAAGGTGGCGCAGATCTACTCCGCTATCCAGACCGGCGGCAACCTGGGCATGCAGACACTGAACTCCTCGTTG
GAACGGCTGGTGAAAGAGGGTGTGGTCAGCGTCGAAGAGGCACAAGCCCGTGCCAAGGGCGACTTGGCTTTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

77.876

98.547

0.767
  pilT Pseudomonas aeruginosa PAK

77.581

98.547

0.765
  pilT Acinetobacter nosocomialis M2

76.401

98.547

0.753
  pilT Acinetobacter baumannii D1279779

76.401

98.547

0.753
  pilT Acinetobacter baumannii strain A118

76.401

98.547

0.753
  pilT Acinetobacter baylyi ADP1

75.516

98.547

0.744
  pilT Legionella pneumophila strain Lp02

71.006

98.256

0.698
  pilT Legionella pneumophila strain ERS1305867

71.006

98.256

0.698
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.006

99.419

0.686
  pilT Vibrio cholerae strain A1552

69.006

99.419

0.686
  pilT Neisseria meningitidis 8013

66.276

99.128

0.657
  pilT Neisseria gonorrhoeae MS11

65.982

99.128

0.654
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.097

98.547

0.523
  pilU Pseudomonas stutzeri DSM 10701

40.634

100

0.41
  pilU Vibrio cholerae strain A1552

39.769

100

0.401
  pilU Acinetobacter baylyi ADP1

40.294

98.837

0.398