Detailed information NTG0899022

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: R5M92_RS13000
• Genome accession: NZ_CP137552
• Coordinates: 2838475..2839635 (-)
• Length: 386 a.a.
• NCBI ID: WP_346796379.1
• Uniprot ID: -
• Organism: Halomonas kashgarensis strain zp-37
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2833475..2844635

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R5M92_RS12990 (R5M92_12990)	tyrS	2836021..2837220 (-)	1200	WP_346796376.1	tyrosine--tRNA ligase	-
R5M92_RS12995 (R5M92_12995)	-	2837338..2838474 (+)	1137	WP_346796378.1	anhydro-N-acetylmuramic acid kinase	-
R5M92_RS13000 (R5M92_13000)	pilU	2838475..2839635 (-)	1161	WP_346796379.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
R5M92_RS13005 (R5M92_13005)	pilT	2839672..2840706 (-)	1035	WP_346796380.1	type IV pilus twitching motility protein PilT	Machinery gene
R5M92_RS13010 (R5M92_13010)	-	2840741..2841454 (+)	714	WP_346796381.1	YggS family pyridoxal phosphate-dependent enzyme	-
R5M92_RS13015 (R5M92_13015)	proC	2841509..2842333 (+)	825	WP_346796382.1	pyrroline-5-carboxylate reductase	-
R5M92_RS13020 (R5M92_13020)	-	2842363..2842956 (+)	594	WP_346796383.1	YggT family protein	-
R5M92_RS13025 (R5M92_13025)	metX	2842988..2844169 (+)	1182	WP_346796384.1	homoserine O-succinyltransferase MetX	-

Sequence

Protein

• Length: 386 a.a.
• Molecular weight: 42583.91 Da
• Isoelectric Point: 6.3417

>NTDB_id=899022 R5M92_RS13000 WP_346796379.1 2838475..2839635(-) (pilU) [Halomonas kashgarensis strain zp-37]
MTEAHEGSLTPIQWLHQLLDIMVTQQASDLLISAGAPPSLKMAQGLVPLGKHCLSQHQVSELVGVTLPEGLRGRFHQDRE
ANFALSLGEKGRFRVSAFQQRNQMAMVIRRISLEIPRLDELGVPPQLADLAQAKRGLVLVVGGSGTGKSTTLAAMIQQRN
ETIGGHIISVEDPIEYIHPHKKAIVNQREVGIDTESFEVALKNTLRQAPDVILIGEIRTRETMQHALTFAETGHLCLATL
HANNANQALDRIINFFPHERHEQIWMDLSLNLKAIVAQQLMPRVGGGRYPAIEIMLQSPRIADLISKGAVGEIKPAMAKS
RDGGMQTFDQALFDLYRADLISEEVALAHADSANDLRMMIKFGEHRDVVPAAEAPSQLALRDEEDY

Nucleotide

• Length: 1161 bp

>NTDB_id=899022 R5M92_RS13000 WP_346796379.1 2838475..2839635(-) (pilU) [Halomonas kashgarensis strain zp-37]
ATGACTGAAGCTCACGAGGGAAGCCTGACGCCGATCCAGTGGCTGCATCAACTGCTCGATATCATGGTGACACAGCAGGC
GTCGGACCTTTTGATTTCGGCGGGTGCCCCGCCGAGTTTGAAGATGGCTCAGGGCCTGGTACCGCTGGGCAAGCACTGCC
TGAGCCAGCATCAGGTCAGCGAGTTGGTCGGTGTCACGCTGCCGGAGGGGCTGCGGGGGCGGTTCCATCAGGACCGAGAG
GCGAATTTCGCCCTGAGCCTGGGCGAAAAGGGGCGCTTTCGCGTGAGTGCCTTCCAGCAGCGCAACCAGATGGCGATGGT
GATCCGGCGCATCTCGCTGGAAATCCCCCGGCTGGATGAGCTGGGCGTCCCGCCGCAACTGGCCGACCTGGCGCAGGCCA
AGCGCGGCCTGGTACTGGTGGTGGGAGGGAGCGGCACAGGGAAATCGACGACCTTGGCAGCGATGATCCAGCAGCGCAAC
GAGACCATCGGTGGGCATATCATCAGTGTGGAAGATCCCATCGAATATATTCACCCGCACAAGAAGGCCATCGTCAATCA
GCGAGAAGTCGGCATCGATACCGAGTCCTTCGAGGTGGCGCTGAAGAACACCCTGCGCCAGGCGCCGGATGTGATTCTGA
TCGGCGAGATTCGCACCCGGGAAACCATGCAGCATGCCTTGACCTTTGCTGAAACCGGACACCTGTGCCTGGCGACGCTG
CACGCCAACAATGCCAACCAGGCGCTCGATCGTATCATCAACTTCTTTCCTCACGAGCGTCATGAACAGATATGGATGGA
CCTATCGCTCAATCTCAAGGCGATCGTGGCGCAGCAGCTCATGCCCAGGGTGGGCGGCGGTCGCTATCCGGCCATCGAGA
TCATGTTGCAGTCTCCGCGTATCGCCGACCTGATCAGCAAGGGGGCAGTGGGAGAAATCAAGCCGGCGATGGCAAAGTCC
CGTGATGGCGGGATGCAGACCTTCGATCAGGCGCTTTTTGATCTCTACCGTGCCGATCTCATCAGCGAGGAAGTGGCCCT
GGCGCATGCCGATTCCGCCAACGATCTGCGCATGATGATCAAGTTTGGCGAGCATAGGGATGTTGTGCCCGCCGCGGAGG
CGCCGAGCCAGCTGGCCCTGCGCGACGAAGAAGATTACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (81-282)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.299	91.71	0.562
pilU	Vibrio cholerae strain A1552	58.046	90.155	0.523
pilU	Acinetobacter baylyi ADP1	55.682	91.192	0.508
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	44.214	87.306	0.386