Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   R3L12_RS08520 Genome accession   NZ_CP137057
Coordinates   1812533..1813567 (+) Length   344 a.a.
NCBI ID   WP_149930465.1    Uniprot ID   -
Organism   Acinetobacter soli strain SNUBHAB0075     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1807533..1818567
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R3L12_RS08505 (R3L12_08485) - 1809878..1810669 (+) 792 WP_149930467.1 transglutaminase family protein -
  R3L12_RS08510 (R3L12_08490) - 1810764..1811609 (-) 846 WP_149930466.1 undecaprenyl-diphosphate phosphatase -
  R3L12_RS08515 (R3L12_08495) - 1811715..1812407 (-) 693 WP_088459378.1 YggS family pyridoxal phosphate-dependent enzyme -
  R3L12_RS08520 (R3L12_08500) pilT 1812533..1813567 (+) 1035 WP_149930465.1 type IV pilus twitching motility protein PilT Machinery gene
  R3L12_RS08525 (R3L12_08505) pilU 1813600..1814751 (+) 1152 WP_149930464.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  R3L12_RS08530 (R3L12_08510) fur 1814803..1815240 (-) 438 WP_004935475.1 ferric iron uptake transcriptional regulator -
  R3L12_RS08535 (R3L12_08515) - 1815352..1815750 (+) 399 WP_004935473.1 outer membrane protein assembly factor BamE -
  R3L12_RS08540 (R3L12_08520) - 1815806..1816117 (-) 312 WP_076032905.1 RnfH family protein -
  R3L12_RS08545 (R3L12_08525) - 1816114..1817202 (-) 1089 WP_171490486.1 hypothetical protein -
  R3L12_RS08550 (R3L12_08530) - 1817342..1818190 (-) 849 WP_076032907.1 class II glutamine amidotransferase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38307.00 Da        Isoelectric Point: 6.4335

>NTDB_id=894458 R3L12_RS08520 WP_149930465.1 1812533..1813567(+) (pilT) [Acinetobacter soli strain SNUBHAB0075]
MDITELLAFSVQNRASDLHLSAGLAPMIRVDGEVRRINLPALEHSEVYRLVYDIMNDKQRRDYEDKLETDFSFEVPNLAR
FRVNVFNQNRGAGAVFRTIPSQVLTLEELGLGKIFRDICDYPRGLVLVTGPTGSGKSTTLAAMLDYINHHRYDHILTVED
PIEFVHEPKKCLINQREVHRDTLGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPAEEKDMVRTMLSESLQAVISQTLLKKEGGGRVAAHEIMIGIPAIRNLIREGKVAQMYSSIQTGASHGMTTLDQSL
KQLVAKGIINLPTARAVAKSPESL

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=894458 R3L12_RS08520 WP_149930465.1 1812533..1813567(+) (pilT) [Acinetobacter soli strain SNUBHAB0075]
ATGGATATAACCGAACTGCTGGCATTTTCAGTCCAAAACAGGGCATCTGATTTACATTTGTCTGCAGGTTTGGCACCCAT
GATTCGCGTCGATGGTGAAGTTCGACGCATTAACCTCCCCGCACTTGAACATTCCGAAGTATACCGGCTGGTTTACGACA
TTATGAATGACAAACAGCGTCGCGATTACGAAGATAAGCTCGAAACCGACTTTTCCTTTGAGGTGCCAAATCTGGCCCGT
TTTCGTGTCAATGTGTTTAATCAGAACCGTGGTGCAGGTGCCGTTTTTCGAACCATTCCCTCACAGGTATTGACGCTTGA
AGAACTCGGACTCGGAAAAATTTTCAGAGACATCTGTGACTATCCGCGCGGCTTGGTGTTAGTGACCGGCCCAACAGGCT
CGGGGAAGTCGACCACCCTTGCCGCCATGCTCGATTATATTAATCATCATCGCTATGATCATATTTTAACGGTTGAAGAT
CCGATCGAGTTTGTACACGAACCCAAAAAATGCCTAATCAACCAGCGCGAAGTGCACCGCGACACACTTGGTTTTAATGA
GGCACTTCGCTCAGCACTGCGTGAAGATCCTGATATCATTCTGGTGGGGGAAATGCGTGATCTGGAAACCATACGTTTGG
CATTGACTGCCGCAGAAACCGGACATTTGGTGTTTGGTACGCTACACACGACCTCGGCAGCCAAAACCATTGATCGAATT
ATCGATGTATTTCCCGCAGAGGAAAAAGATATGGTTCGTACCATGCTGTCCGAATCCCTTCAGGCGGTTATTTCACAAAC
CTTGTTAAAAAAAGAAGGTGGCGGTCGTGTGGCAGCACATGAAATTATGATTGGTATTCCGGCCATTCGTAATTTGATCC
GTGAAGGAAAAGTCGCGCAAATGTATTCTTCGATTCAAACGGGTGCAAGCCATGGCATGACCACGCTCGATCAAAGTCTT
AAACAACTGGTCGCCAAAGGCATCATTAACTTACCAACAGCACGCGCTGTGGCAAAATCACCAGAATCCTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

96.512

100

0.965
  pilT Acinetobacter baumannii D1279779

89.244

100

0.892
  pilT Acinetobacter baumannii strain A118

89.244

100

0.892
  pilT Acinetobacter nosocomialis M2

88.953

100

0.89
  pilT Pseudomonas stutzeri DSM 10701

81.395

100

0.814
  pilT Pseudomonas aeruginosa PAK

81.395

100

0.814
  pilT Legionella pneumophila strain Lp02

74.26

98.256

0.73
  pilT Legionella pneumophila strain ERS1305867

74.26

98.256

0.73
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.515

95.93

0.686
  pilT Vibrio cholerae strain A1552

71.515

95.93

0.686
  pilT Neisseria meningitidis 8013

67.246

100

0.674
  pilT Neisseria gonorrhoeae MS11

66.957

100

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.333

95.93

0.512
  pilU Vibrio cholerae strain A1552

41.739

100

0.419
  pilU Pseudomonas stutzeri DSM 10701

38.841

100

0.39
  pilU Acinetobacter baylyi ADP1

38.15

100

0.384