Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   R3L12_RS08525 Genome accession   NZ_CP137057
Coordinates   1813600..1814751 (+) Length   383 a.a.
NCBI ID   WP_149930464.1    Uniprot ID   -
Organism   Acinetobacter soli strain SNUBHAB0075     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1808600..1819751
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R3L12_RS08505 (R3L12_08485) - 1809878..1810669 (+) 792 WP_149930467.1 transglutaminase family protein -
  R3L12_RS08510 (R3L12_08490) - 1810764..1811609 (-) 846 WP_149930466.1 undecaprenyl-diphosphate phosphatase -
  R3L12_RS08515 (R3L12_08495) - 1811715..1812407 (-) 693 WP_088459378.1 YggS family pyridoxal phosphate-dependent enzyme -
  R3L12_RS08520 (R3L12_08500) pilT 1812533..1813567 (+) 1035 WP_149930465.1 type IV pilus twitching motility protein PilT Machinery gene
  R3L12_RS08525 (R3L12_08505) pilU 1813600..1814751 (+) 1152 WP_149930464.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  R3L12_RS08530 (R3L12_08510) fur 1814803..1815240 (-) 438 WP_004935475.1 ferric iron uptake transcriptional regulator -
  R3L12_RS08535 (R3L12_08515) - 1815352..1815750 (+) 399 WP_004935473.1 outer membrane protein assembly factor BamE -
  R3L12_RS08540 (R3L12_08520) - 1815806..1816117 (-) 312 WP_076032905.1 RnfH family protein -
  R3L12_RS08545 (R3L12_08525) - 1816114..1817202 (-) 1089 WP_171490486.1 hypothetical protein -
  R3L12_RS08550 (R3L12_08530) - 1817342..1818190 (-) 849 WP_076032907.1 class II glutamine amidotransferase -
  R3L12_RS08555 (R3L12_08535) - 1818291..1819076 (-) 786 WP_149930462.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 383 a.a.        Molecular weight: 43218.49 Da        Isoelectric Point: 6.5598

>NTDB_id=894459 R3L12_RS08525 WP_149930464.1 1813600..1814751(+) (pilU) [Acinetobacter soli strain SNUBHAB0075]
MDFNDLLNLMASHKASDMFITAGVEPSLKINGKITPVSKTKLDGDMIAQLIDSITTQKQRDEYMATHECNFAILNRERTE
RFRVSAFQQRDLPGMVIRRIETKIPTLDQLQLPPILKDLALTKRGIILFSGATGTGKSTSLASMIHHRNLHSSGHIITIE
DPIEFIHAHAGCIVTQREVGIDTDSYEVALKNTLRQAPDVILIGEIRTREVMDYALAFAETGHLVFATLHANNANQTLER
IIHFFEQDRREQVLMDLSLNLKAVISQQLIATPDGQSRRAAVEVMINSPLMSDYIRQGEIYKIKELMARSGELGMQTFDQ
ALFELHEHGQISYADALKHADSPNNLRLQIKLSQEANTAIDQKEKMPEVDRKITFDGQRHDLK

Nucleotide


Download         Length: 1152 bp        

>NTDB_id=894459 R3L12_RS08525 WP_149930464.1 1813600..1814751(+) (pilU) [Acinetobacter soli strain SNUBHAB0075]
ATGGACTTTAATGATTTACTCAATTTAATGGCATCCCACAAAGCCTCCGATATGTTCATTACGGCTGGTGTTGAGCCATC
ATTAAAAATTAATGGCAAAATTACCCCAGTTTCCAAAACCAAATTAGACGGTGACATGATCGCGCAGTTGATTGATTCGA
TCACCACGCAAAAGCAACGCGATGAGTACATGGCAACACATGAATGTAATTTCGCCATTTTAAACCGCGAACGTACTGAG
CGCTTTCGTGTAAGTGCCTTTCAGCAGCGTGATTTACCCGGCATGGTTATCCGCCGCATCGAAACCAAAATTCCGACGCT
AGATCAACTACAGCTACCACCGATTCTCAAGGACTTGGCCTTGACTAAACGTGGTATTATTTTGTTTTCTGGTGCAACAG
GAACAGGAAAATCCACTTCGCTTGCGTCGATGATTCATCACCGTAATCTACACTCAAGCGGTCATATTATTACCATTGAA
GATCCGATTGAATTTATACATGCACATGCTGGCTGTATTGTTACGCAGCGCGAAGTCGGTATTGATACTGATTCCTACGA
AGTGGCTTTAAAAAATACGTTACGCCAAGCACCAGACGTTATTTTGATTGGTGAAATCCGCACACGTGAAGTCATGGATT
ATGCACTGGCATTTGCCGAAACCGGGCATTTGGTTTTTGCAACTTTACATGCCAACAACGCCAATCAGACCTTAGAGCGT
ATCATTCACTTTTTTGAGCAAGATCGACGTGAGCAGGTATTGATGGACTTATCACTTAACTTAAAAGCCGTCATCTCTCA
GCAACTGATTGCTACGCCTGATGGTCAAAGTCGCCGTGCTGCCGTTGAAGTGATGATCAATTCACCTTTGATGTCGGATT
ACATTCGTCAAGGTGAAATTTATAAAATTAAGGAACTGATGGCGCGTTCTGGTGAACTGGGAATGCAGACCTTTGATCAG
GCCTTGTTTGAACTACACGAACACGGACAAATCAGTTATGCCGATGCACTCAAACATGCCGACTCACCCAACAATCTGCG
TTTGCAGATCAAACTTTCTCAAGAAGCCAATACCGCAATTGATCAAAAAGAAAAAATGCCTGAAGTCGATCGAAAAATCA
CCTTTGACGGTCAACGTCATGATCTAAAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

85.64

100

0.856
  pilU Pseudomonas stutzeri DSM 10701

66.106

93.211

0.616
  pilU Vibrio cholerae strain A1552

55.556

91.645

0.509
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.42

95.822

0.368