Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   R2X36_RS02855 Genome accession   NZ_CP137009
Coordinates   581937..582971 (-) Length   344 a.a.
NCBI ID   WP_041206426.1    Uniprot ID   -
Organism   Aeromonas media strain QST31     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 576937..587971
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R2X36_RS02835 (R2X36_02835) - 577747..578457 (-) 711 WP_042649785.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  R2X36_RS02840 (R2X36_02840) srmB 578586..579809 (+) 1224 WP_005332263.1 ATP-dependent RNA helicase SrmB -
  R2X36_RS02845 (R2X36_02845) yaaA 579995..580768 (-) 774 WP_171272666.1 peroxide stress protein YaaA -
  R2X36_RS02850 (R2X36_02850) pilU 580801..581910 (-) 1110 WP_268422625.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  R2X36_RS02855 (R2X36_02855) pilT 581937..582971 (-) 1035 WP_041206426.1 type IVa pilus ATPase TapT Machinery gene
  R2X36_RS02860 (R2X36_02860) - 583011..583712 (+) 702 WP_317841900.1 YggS family pyridoxal phosphate-dependent enzyme -
  R2X36_RS02865 (R2X36_02865) proC 583872..584699 (+) 828 WP_317841901.1 pyrroline-5-carboxylate reductase -
  R2X36_RS02870 (R2X36_02870) - 584723..585274 (+) 552 WP_025325667.1 YggT family protein -
  R2X36_RS02875 (R2X36_02875) yggU 585274..585582 (+) 309 WP_043128939.1 DUF167 family protein YggU -
  R2X36_RS02880 (R2X36_02880) - 585587..586006 (+) 420 WP_041206420.1 DUF4426 domain-containing protein -
  R2X36_RS02885 (R2X36_02885) - 586086..586916 (-) 831 WP_171269314.1 CPBP family intramembrane glutamic endopeptidase -
  R2X36_RS02890 (R2X36_02890) - 587111..587407 (-) 297 WP_005332293.1 hypothetical protein -
  R2X36_RS02895 (R2X36_02895) - 587458..587832 (-) 375 WP_005332296.1 YacL family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38257.00 Da        Isoelectric Point: 6.6958

>NTDB_id=894213 R2X36_RS02855 WP_041206426.1 581937..582971(-) (pilT) [Aeromonas media strain QST31]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALDHREVHALIYDIMNDYQRKELEENFEVDFSFEVPGMAR
FRVNAFQQARGSGAVFRTIPSTVLTLEDLEAPEMFRKIAEFPRGLVLVTGPTGSGKSTTLAAMVNYINDNFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFANALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMLGIPAVRNLIREDKVAQLYSVIQTGMTHGMQTMDQSL
KQLVNRGVVAALDARAKAADPNSI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=894213 R2X36_RS02855 WP_041206426.1 581937..582971(-) (pilT) [Aeromonas media strain QST31]
ATGGATATCACAGAGTTATTGGCTTTCAGTGTAAAGCATAAGGCCTCGGATCTACACCTCTCAGCCGGGGTTCCCCCGAT
GATCAGGGTTGATGGAGAGGTTCGCAAGATCAATTTACCCGCCCTGGATCACCGTGAAGTGCACGCCCTGATCTACGACA
TCATGAACGACTATCAGCGCAAGGAGCTGGAGGAGAATTTCGAGGTGGACTTCTCCTTCGAGGTACCCGGCATGGCGCGT
TTTCGGGTCAACGCCTTTCAACAGGCGCGGGGCTCTGGCGCCGTGTTTCGCACCATACCCAGTACCGTTCTGACCCTGGA
GGATCTGGAAGCTCCCGAGATGTTCCGCAAGATAGCCGAGTTTCCGCGCGGCCTGGTGCTGGTCACGGGCCCGACCGGTT
CGGGCAAGTCCACCACGCTTGCGGCCATGGTCAATTACATCAATGACAACTTCCACCACCACATCCTCACCATCGAGGAT
CCCATCGAGTTCGTGCACGAGAACAAGCGCTGCCTGGTCAACCAGCGCGAGGTGCACCGCGATACCAAGAGTTTCGCCAA
CGCCCTCCGCTCGGCCCTGCGTGAGGACCCGGACATCATACTGGTGGGGGAAATGCGCGACCTCGAGACCATCCGCCTCG
CCATGACGGCGGCAGAGACCGGTCATCTGGTGTTCGGTACCCTGCACACCTCGTCGGCGGCCAAGACCATAGACCGGATC
ATCGACGTCTTCCCCGGCTCGGAGAAGGACATGGTGAGATCCATGCTCTCCGAGTCCCTGCGGGCGGTCATCTCCCAGAC
TCTGCTCAAGCGGATCGGCGGTGGCCGGGTGGCAGCCCACGAGATCATGCTGGGGATCCCGGCGGTGCGAAACCTCATCC
GGGAAGACAAGGTCGCCCAACTCTACTCCGTCATCCAGACCGGGATGACCCACGGCATGCAAACCATGGATCAGAGCCTC
AAGCAGCTCGTCAACCGAGGCGTGGTTGCCGCCCTCGATGCCAGGGCCAAGGCGGCCGATCCCAACAGCATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

77.843

99.709

0.776
  pilT Acinetobacter baumannii D1279779

76.968

99.709

0.767
  pilT Acinetobacter baumannii strain A118

76.968

99.709

0.767
  pilT Acinetobacter nosocomialis M2

76.968

99.709

0.767
  pilT Pseudomonas stutzeri DSM 10701

76.744

100

0.767
  pilT Pseudomonas aeruginosa PAK

75.872

100

0.759
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

76.558

97.965

0.75
  pilT Vibrio cholerae strain A1552

76.558

97.965

0.75
  pilT Legionella pneumophila strain Lp02

72.727

95.93

0.698
  pilT Legionella pneumophila strain ERS1305867

72.727

95.93

0.698
  pilT Neisseria meningitidis 8013

68.696

100

0.689
  pilT Neisseria gonorrhoeae MS11

68.406

100

0.686
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.339

99.419

0.52
  pilU Vibrio cholerae strain A1552

43.478

100

0.436
  pilU Pseudomonas stutzeri DSM 10701

41.449

100

0.416
  pilU Acinetobacter baylyi ADP1

39.884

100

0.401