Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   R2X36_RS02850 Genome accession   NZ_CP137009
Coordinates   580801..581910 (-) Length   369 a.a.
NCBI ID   WP_268422625.1    Uniprot ID   -
Organism   Aeromonas media strain QST31     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 575801..586910
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R2X36_RS02825 (R2X36_02825) - 575932..576159 (+) 228 WP_005332257.1 hypothetical protein -
  R2X36_RS02830 (R2X36_02830) brnQ 576248..577510 (-) 1263 WP_317842629.1 branched-chain amino acid transport system II carrier protein -
  R2X36_RS02835 (R2X36_02835) - 577747..578457 (-) 711 WP_042649785.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  R2X36_RS02840 (R2X36_02840) srmB 578586..579809 (+) 1224 WP_005332263.1 ATP-dependent RNA helicase SrmB -
  R2X36_RS02845 (R2X36_02845) yaaA 579995..580768 (-) 774 WP_171272666.1 peroxide stress protein YaaA -
  R2X36_RS02850 (R2X36_02850) pilU 580801..581910 (-) 1110 WP_268422625.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  R2X36_RS02855 (R2X36_02855) pilT 581937..582971 (-) 1035 WP_041206426.1 type IVa pilus ATPase TapT Machinery gene
  R2X36_RS02860 (R2X36_02860) - 583011..583712 (+) 702 WP_317841900.1 YggS family pyridoxal phosphate-dependent enzyme -
  R2X36_RS02865 (R2X36_02865) proC 583872..584699 (+) 828 WP_317841901.1 pyrroline-5-carboxylate reductase -
  R2X36_RS02870 (R2X36_02870) - 584723..585274 (+) 552 WP_025325667.1 YggT family protein -
  R2X36_RS02875 (R2X36_02875) yggU 585274..585582 (+) 309 WP_043128939.1 DUF167 family protein YggU -
  R2X36_RS02880 (R2X36_02880) - 585587..586006 (+) 420 WP_041206420.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41031.16 Da        Isoelectric Point: 6.1858

>NTDB_id=894212 R2X36_RS02850 WP_268422625.1 580801..581910(-) (pilU) [Aeromonas media strain QST31]
MNMDVLLAALVEQKGSDLFITVDAPPTLKVNGRLVPLGDKPLDRQTALTLVRDSLDETHFERYLHSREANYAIHREGLGR
FRVSAFWQQDMPGMVLRRIETRIPTFEELVLPPILQDVAMAKRGLVLFVGATGAGKSTTQAAMIGYRNQHGDGHILTVED
PVEFVHQHGRCLVTQREVGIDTDSFDVALKNSMRQAPDVILIGEIRTQETMELAIQFAETGHLCLATLHANNANQALDRI
LHLVPEAKHRQFLFDLSFNLRAIVAQQLLPSVNGSRRIAAFEILLNTPLITDIIRKGEMHRLKEVMTKSGELGMQTFDQA
LFTLFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=894212 R2X36_RS02850 WP_268422625.1 580801..581910(-) (pilU) [Aeromonas media strain QST31]
ATGAACATGGATGTTCTGCTCGCTGCACTGGTTGAACAAAAGGGATCGGATCTCTTCATCACGGTCGATGCCCCCCCCAC
CCTCAAGGTCAATGGACGCCTGGTACCACTGGGCGATAAGCCGCTGGACAGGCAGACGGCGCTGACCCTGGTCAGGGATA
GCCTGGACGAGACGCACTTCGAGCGATACCTGCATAGCCGGGAGGCAAACTACGCCATCCACCGCGAAGGACTTGGCCGA
TTCCGGGTCAGCGCCTTCTGGCAGCAGGATATGCCGGGCATGGTGCTGCGTCGTATCGAGACCCGTATTCCCACCTTCGA
GGAATTGGTACTGCCCCCCATCCTGCAGGATGTGGCCATGGCCAAACGCGGGCTGGTGCTGTTCGTCGGTGCCACGGGGG
CTGGCAAGTCCACCACCCAGGCGGCCATGATCGGTTATCGCAACCAGCACGGCGACGGCCACATACTGACGGTGGAGGAT
CCGGTGGAGTTCGTCCACCAGCATGGGCGCTGCCTGGTGACCCAGCGGGAGGTCGGCATAGATACCGACTCCTTCGACGT
GGCCCTCAAGAATTCCATGCGCCAGGCACCGGATGTCATCCTGATCGGTGAGATCCGTACCCAGGAAACCATGGAACTGG
CCATCCAGTTTGCCGAGACGGGCCACCTCTGCCTCGCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
CTCCATCTGGTGCCGGAGGCGAAACACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGCGCCATCGTGGCGCAGCA
ACTGCTCCCCAGCGTGAATGGCAGCCGCCGGATCGCCGCCTTCGAGATCCTGCTCAACACCCCGCTCATCACCGACATCA
TCCGCAAGGGCGAGATGCACAGGCTCAAGGAGGTGATGACCAAATCCGGCGAGCTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCACCCTGTTCTGCGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCCGCCAACGACCTGCGACT
GCTGATCAAGCTGTCGGGTCGCGAGCAACTGGGTGCAGGCACACTCGACAACGTGACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(119-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

59.511

99.729

0.593
  pilU Pseudomonas stutzeri DSM 10701

60.399

95.122

0.575
  pilU Acinetobacter baylyi ADP1

56.374

95.664

0.539
  pilT Legionella pneumophila strain ERS1305867

41.108

92.954

0.382
  pilT Legionella pneumophila strain Lp02

41.108

92.954

0.382
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.692

89.702

0.374
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363
  pilT Vibrio cholerae strain A1552

39.82

90.515

0.36
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.515

0.36