Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   R0137_RS02865 Genome accession   NZ_CP136865
Coordinates   637815..638849 (+) Length   344 a.a.
NCBI ID   WP_405272373.1    Uniprot ID   -
Organism   Congregibacter brevis strain IMCC45268     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 632815..643849
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R0137_RS02835 (R0137_02825) - 633249..633713 (-) 465 WP_407328385.1 DUF4426 domain-containing protein -
  R0137_RS02840 (R0137_02830) metW 633734..634324 (-) 591 WP_407328387.1 methionine biosynthesis protein MetW -
  R0137_RS02845 (R0137_02835) - 634324..635445 (-) 1122 WP_407328389.1 homoserine O-acetyltransferase -
  R0137_RS02850 (R0137_02840) - 635460..636047 (-) 588 WP_407328391.1 YggT family protein -
  R0137_RS02855 (R0137_02845) proC 636124..636942 (-) 819 WP_407328393.1 pyrroline-5-carboxylate reductase -
  R0137_RS02860 (R0137_02850) - 637005..637697 (-) 693 WP_407328394.1 YggS family pyridoxal phosphate-dependent enzyme -
  R0137_RS02865 (R0137_02855) pilT 637815..638849 (+) 1035 WP_405272373.1 type IV pilus twitching motility protein PilT Machinery gene
  R0137_RS02870 (R0137_02860) pilU 638900..640009 (+) 1110 WP_407328396.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  R0137_RS02875 (R0137_02865) ruvX 640011..640433 (-) 423 WP_407328398.1 Holliday junction resolvase RuvX -
  R0137_RS02880 (R0137_02870) - 640441..641037 (-) 597 WP_407328399.1 YqgE/AlgH family protein -
  R0137_RS02885 (R0137_02875) - 641079..641930 (-) 852 WP_407328401.1 TonB family protein -
  R0137_RS02890 (R0137_02880) gshB 641934..642890 (-) 957 WP_407328402.1 glutathione synthase -
  R0137_RS02895 (R0137_02885) - 642949..643686 (-) 738 WP_407328403.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38421.23 Da        Isoelectric Point: 6.5069

>NTDB_id=893174 R0137_RS02865 WP_405272373.1 637815..638849(+) (pilT) [Congregibacter brevis strain IMCC45268]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPLEHKQVHDLIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGGVFRTIPSKVLTMEDLGMGQVFRDISMMPRGLVLVTGPTGSGKSTTLAAMIDFINDHKYEHILTIED
PIEFVHESRKCLVNQREVHRDTLGFAEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAAEKSMVRSMLSESLQAVVSQTLMKRTAGGRVAAHEIMRATSAIRNLIREDKVAQMYSAIQTGQAVGMQTMDQCL
KELVDKRVISRDVAREKARMPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=893174 R0137_RS02865 WP_405272373.1 637815..638849(+) (pilT) [Congregibacter brevis strain IMCC45268]
ATGGATATTACTGAACTGCTGGCGTTCAGCGCCAAACAGGGAGCTTCGGATTTGCACCTTTCCGCAGGCCTGCCGCCCAT
GATCCGCGTTGATGGCGATGTACGTCGCATCAACTTGCCGCCGCTTGAACACAAGCAGGTGCACGATCTTATCTACGACA
TCATGAACGATAAGCAGCGAAAGGACTTTGAGGAATTTCTGGAGACAGACTTTTCTTTCGAGGTTCCCGGCGTGGCACGC
TTCCGGGTCAATGCCTTTAACCAGAATCGTGGTGCGGGTGGCGTTTTTCGAACCATTCCCTCCAAGGTTCTCACCATGGA
AGACCTGGGCATGGGCCAGGTGTTTCGTGACATCAGCATGATGCCTCGGGGTCTGGTCCTGGTGACGGGGCCAACGGGCT
CAGGAAAGTCCACAACTCTTGCCGCGATGATCGACTTTATTAACGATCACAAGTACGAGCATATTCTCACCATTGAGGAT
CCTATCGAATTTGTTCACGAGAGCCGGAAGTGTCTGGTGAACCAACGGGAAGTTCATCGTGACACCTTAGGCTTTGCCGA
GGCACTGCGCAGCGCTTTGCGAGAAGACCCCGATATTATTCTGGTGGGCGAGATGCGTGACCTGGAGACCATTCGTCTTG
CCCTCACAGCAGCGGAAACCGGTCACCTCGTGTTTGGCACCCTGCACACCACTTCAGCAGCGAAGACGATTGACCGGGTG
ATTGACGTTTTCCCCGCCGCTGAAAAGTCCATGGTGCGTTCAATGCTCTCTGAGTCCCTGCAGGCCGTGGTATCCCAAAC
GCTGATGAAGCGTACTGCCGGTGGGCGTGTGGCAGCGCACGAAATCATGCGCGCTACCTCGGCGATACGTAACCTCATTC
GCGAAGACAAAGTTGCGCAGATGTACTCTGCTATTCAGACCGGCCAGGCCGTGGGCATGCAGACTATGGACCAGTGTCTT
AAGGAGCTGGTCGACAAGCGGGTAATTTCCCGCGACGTTGCGCGAGAGAAAGCGCGCATGCCTGAGAATTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

87.791

100

0.878
  pilT Pseudomonas stutzeri DSM 10701

87.209

100

0.872
  pilT Acinetobacter nosocomialis M2

81.686

100

0.817
  pilT Acinetobacter baumannii D1279779

81.686

100

0.817
  pilT Acinetobacter baumannii strain A118

81.686

100

0.817
  pilT Acinetobacter baylyi ADP1

78.779

100

0.788
  pilT Legionella pneumophila strain Lp02

76.036

98.256

0.747
  pilT Legionella pneumophila strain ERS1305867

76.036

98.256

0.747
  pilT Neisseria meningitidis 8013

66.377

100

0.666
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.301

95.64

0.663
  pilT Vibrio cholerae strain A1552

69.301

95.64

0.663
  pilT Neisseria gonorrhoeae MS11

65.797

100

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.333

100

0.535
  pilU Acinetobacter baylyi ADP1

39.595

100

0.398
  pilU Pseudomonas stutzeri DSM 10701

41.358

94.186

0.39
  pilU Vibrio cholerae strain A1552

39.521

97.093

0.384