Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   R0137_RS02870 Genome accession   NZ_CP136865
Coordinates   638900..640009 (+) Length   369 a.a.
NCBI ID   WP_407328396.1    Uniprot ID   -
Organism   Congregibacter brevis strain IMCC45268     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 633900..645009
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R0137_RS02845 (R0137_02835) - 634324..635445 (-) 1122 WP_407328389.1 homoserine O-acetyltransferase -
  R0137_RS02850 (R0137_02840) - 635460..636047 (-) 588 WP_407328391.1 YggT family protein -
  R0137_RS02855 (R0137_02845) proC 636124..636942 (-) 819 WP_407328393.1 pyrroline-5-carboxylate reductase -
  R0137_RS02860 (R0137_02850) - 637005..637697 (-) 693 WP_407328394.1 YggS family pyridoxal phosphate-dependent enzyme -
  R0137_RS02865 (R0137_02855) pilT 637815..638849 (+) 1035 WP_405272373.1 type IV pilus twitching motility protein PilT Machinery gene
  R0137_RS02870 (R0137_02860) pilU 638900..640009 (+) 1110 WP_407328396.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  R0137_RS02875 (R0137_02865) ruvX 640011..640433 (-) 423 WP_407328398.1 Holliday junction resolvase RuvX -
  R0137_RS02880 (R0137_02870) - 640441..641037 (-) 597 WP_407328399.1 YqgE/AlgH family protein -
  R0137_RS02885 (R0137_02875) - 641079..641930 (-) 852 WP_407328401.1 TonB family protein -
  R0137_RS02890 (R0137_02880) gshB 641934..642890 (-) 957 WP_407328402.1 glutathione synthase -
  R0137_RS02895 (R0137_02885) - 642949..643686 (-) 738 WP_407328403.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  R0137_RS02900 (R0137_02890) metF 643696..644532 (-) 837 WP_407328405.1 methylenetetrahydrofolate reductase [NAD(P)H] -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40401.32 Da        Isoelectric Point: 6.5535

>NTDB_id=893175 R0137_RS02870 WP_407328396.1 638900..640009(+) (pilU) [Congregibacter brevis strain IMCC45268]
MQIDDLLAKMVESGASDGFITARAAPSIKVDGEIHAIGKDGLSLEQTRLLVLETMSPAQQKEFEQNQECNYAVHSDKYGR
FRASAFIQRGQHGLVLRRIKDEIPRIPELGLPPIVAELAMAKRGLVIFVGGTGTGKSTSLAAMVGHRNRNSRGHILSIED
PIEFVHDHAGCLVTQREVGIDTETFEAGLQNALRQAPDVILIGEVRSAETMAHALTFAETGHLCLCTLHANNANQAIDRI
LSFFPVARHHQVLMDLSLNMNAMIAQQLLPTAKGDGRVPVMEVLLNSPLIADHIRNGQVHLIKEVMAKSTELGMQTLDQS
LLAKYCDGEVTADEAIRCADSANEVRLGIKMFDKRRSSHANTVNLQVDR

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=893175 R0137_RS02870 WP_407328396.1 638900..640009(+) (pilU) [Congregibacter brevis strain IMCC45268]
ATGCAAATTGATGACCTGTTAGCCAAGATGGTGGAGTCCGGCGCTTCCGATGGTTTTATAACCGCACGCGCGGCCCCTTC
TATTAAGGTTGACGGTGAGATTCACGCCATTGGCAAAGATGGTTTGAGCCTTGAGCAAACTCGTCTGTTGGTTCTGGAGA
CGATGTCTCCTGCACAGCAGAAAGAGTTTGAACAAAATCAGGAATGCAATTACGCGGTGCACTCTGACAAGTACGGCCGA
TTCCGAGCCAGTGCCTTTATCCAGCGAGGCCAGCACGGTCTGGTTCTGCGTCGTATTAAGGATGAGATTCCGCGAATTCC
AGAGCTGGGTTTACCCCCCATCGTCGCTGAACTCGCGATGGCTAAACGGGGTCTGGTTATTTTTGTGGGAGGTACTGGAA
CCGGTAAGTCGACATCTCTCGCCGCGATGGTAGGACACCGGAATCGCAATAGTCGGGGACACATCCTGTCTATCGAGGAT
CCCATCGAATTTGTGCATGATCACGCAGGTTGTCTCGTCACCCAGCGAGAAGTGGGCATCGATACAGAGACCTTTGAAGC
GGGCCTGCAAAACGCGTTGCGACAGGCTCCCGACGTTATTCTCATCGGTGAAGTGCGCTCAGCGGAGACCATGGCTCATG
CCCTGACTTTCGCTGAAACCGGACATTTGTGTTTATGTACTTTGCACGCCAATAACGCTAATCAGGCTATTGACCGTATT
CTGAGCTTCTTCCCCGTTGCGCGGCATCACCAGGTGCTTATGGATCTGTCGCTGAATATGAACGCCATGATCGCGCAGCA
GCTCTTACCCACCGCAAAGGGCGATGGCCGGGTACCTGTCATGGAAGTTTTGCTCAATTCGCCTCTGATCGCTGATCACA
TTCGAAATGGTCAGGTGCATCTGATCAAAGAAGTCATGGCGAAGTCCACGGAGCTAGGTATGCAGACGCTCGATCAGTCC
CTGCTTGCGAAGTACTGTGATGGCGAAGTGACCGCTGATGAGGCCATACGCTGTGCCGACTCTGCAAACGAAGTGCGCTT
GGGTATCAAGATGTTCGATAAGCGTCGGAGCAGCCACGCGAATACCGTGAACTTGCAGGTTGATCGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

58.791

98.645

0.58
  pilU Acinetobacter baylyi ADP1

54.83

95.393

0.523
  pilU Vibrio cholerae strain A1552

54.571

94.851

0.518
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.816

94.309

0.404
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.773

95.393

0.379
  pilT Vibrio cholerae strain A1552

39.773

95.393

0.379
  pilT Neisseria meningitidis 8013

39.942

92.954

0.371
  pilT Legionella pneumophila strain ERS1305867

42.056

86.992

0.366
  pilT Legionella pneumophila strain Lp02

42.056

86.992

0.366
  pilT Acinetobacter baumannii D1279779

40

90.786

0.363
  pilT Acinetobacter baumannii strain A118

40

90.786

0.363
  pilT Acinetobacter nosocomialis M2

40

90.786

0.363
  pilT Neisseria gonorrhoeae MS11

39.181

92.683

0.363
  pilT Pseudomonas stutzeri DSM 10701

39.701

90.786

0.36
  pilT Pseudomonas aeruginosa PAK

39.701

90.786

0.36