Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   R0135_RS11400 Genome accession   NZ_CP136864
Coordinates   2589827..2590861 (-) Length   344 a.a.
NCBI ID   WP_407346978.1    Uniprot ID   -
Organism   Congregibacter variabilis strain IMCC43200     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2584827..2595861
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R0135_RS11370 (R0135_11330) - 2584972..2585709 (+) 738 WP_407346971.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  R0135_RS11375 (R0135_11335) gshB 2585768..2586724 (+) 957 WP_407346973.1 glutathione synthase -
  R0135_RS11380 (R0135_11340) - 2586728..2587579 (+) 852 WP_407346974.1 TonB family protein -
  R0135_RS11385 (R0135_11345) - 2587698..2588219 (+) 522 WP_407349912.1 YqgE/AlgH family protein -
  R0135_RS11390 (R0135_11350) ruvX 2588221..2588649 (+) 429 WP_407346976.1 Holliday junction resolvase RuvX -
  R0135_RS11395 (R0135_11355) pilU 2588664..2589773 (-) 1110 WP_407346977.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  R0135_RS11400 (R0135_11360) pilT 2589827..2590861 (-) 1035 WP_407346978.1 type IV pilus twitching motility protein PilT Machinery gene
  R0135_RS11405 (R0135_11365) - 2590984..2591676 (+) 693 WP_407346979.1 YggS family pyridoxal phosphate-dependent enzyme -
  R0135_RS11410 (R0135_11370) proC 2591739..2592557 (+) 819 WP_407346980.1 pyrroline-5-carboxylate reductase -
  R0135_RS11415 (R0135_11375) - 2592601..2593188 (+) 588 WP_407346981.1 YggT family protein -
  R0135_RS11420 (R0135_11380) - 2593204..2594334 (+) 1131 WP_407346982.1 homoserine O-acetyltransferase -
  R0135_RS11425 (R0135_11385) metW 2594334..2594924 (+) 591 WP_407346983.1 methionine biosynthesis protein MetW -
  R0135_RS11430 (R0135_11390) - 2594927..2595421 (+) 495 WP_407346984.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38435.26 Da        Isoelectric Point: 6.5069

>NTDB_id=893161 R0135_RS11400 WP_407346978.1 2589827..2590861(-) (pilT) [Congregibacter variabilis strain IMCC43200]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPLEHKQVHDLIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGGVFRTIPSKVLSMEDLGMGQVFRDISMMPRGLVLVTGPTGSGKSTTLAAMIDFINDHKYEHILTIED
PIEFVHESRKCLVNQREVHRDTLGFAEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAAEKSMVRSMLSESLQAVISQTLLKRTAGGRVAAHEIMRATSAIRNLIREDKVAQMYSAIQTGQAVGMQTMDQCL
KEMVDKRVISRDIAREKARMPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=893161 R0135_RS11400 WP_407346978.1 2589827..2590861(-) (pilT) [Congregibacter variabilis strain IMCC43200]
ATGGATATTACCGAACTGCTGGCGTTTAGCGCCAAACAGGGTGCGTCGGATTTGCACCTCTCCGCAGGCCTGCCGCCGAT
GATTCGTGTCGATGGCGATGTACGTCGTATCAACCTGCCTCCACTTGAGCACAAGCAGGTGCACGACCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGACTTCGAAGAATTTCTGGAGACTGATTTCTCCTTTGAAGTACCGGGTGTCGCGCGC
TTTCGTGTAAACGCCTTCAATCAGAACCGCGGTGCCGGCGGCGTGTTTCGAACCATTCCCTCCAAGGTGCTCAGCATGGA
AGACCTGGGCATGGGGCAGGTGTTTCGCGACATCAGCATGATGCCCCGGGGTTTGGTGCTAGTGACTGGTCCGACGGGTT
CAGGAAAGTCTACGACGCTTGCCGCGATGATCGACTTCATCAACGATCACAAATACGAGCACATTCTTACCATTGAGGAC
CCGATCGAATTTGTTCACGAGAGCCGAAAGTGCCTGGTAAACCAGCGCGAGGTCCATCGGGACACGCTGGGCTTTGCTGA
AGCACTGCGTAGCGCACTTAGAGAAGACCCGGATATCATTCTAGTGGGCGAAATGCGTGATCTGGAGACCATTCGCCTGG
CGCTTACCGCCGCCGAGACGGGGCACCTGGTGTTTGGCACCCTACACACCACATCAGCGGCCAAGACCATTGACCGTGTG
ATTGACGTGTTCCCCGCCGCAGAAAAATCCATGGTTCGCTCTATGCTCTCCGAGTCATTGCAGGCCGTAATTTCGCAAAC
CTTGTTGAAGCGTACTGCCGGAGGCCGGGTAGCGGCGCACGAGATCATGCGAGCGACGTCCGCAATTAGAAACCTTATTC
GAGAGGACAAGGTGGCACAGATGTATTCCGCTATCCAAACGGGTCAGGCTGTAGGAATGCAGACCATGGACCAATGCCTC
AAGGAGATGGTCGACAAGCGAGTTATCTCTAGGGATATTGCGCGCGAAAAAGCCCGTATGCCGGAAAACTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

87.5

100

0.875
  pilT Pseudomonas stutzeri DSM 10701

87.209

100

0.872
  pilT Acinetobacter nosocomialis M2

81.686

100

0.817
  pilT Acinetobacter baumannii D1279779

81.686

100

0.817
  pilT Acinetobacter baumannii strain A118

81.686

100

0.817
  pilT Acinetobacter baylyi ADP1

78.779

100

0.788
  pilT Legionella pneumophila strain Lp02

76.036

98.256

0.747
  pilT Legionella pneumophila strain ERS1305867

76.036

98.256

0.747
  pilT Neisseria meningitidis 8013

66.667

100

0.669
  pilT Neisseria gonorrhoeae MS11

66.377

100

0.666
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.301

95.64

0.663
  pilT Vibrio cholerae strain A1552

69.301

95.64

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.043

100

0.532
  pilU Acinetobacter baylyi ADP1

39.884

100

0.401
  pilU Vibrio cholerae strain A1552

39.521

97.093

0.384
  pilU Pseudomonas stutzeri DSM 10701

40.432

94.186

0.381