Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   R0135_RS11395 Genome accession   NZ_CP136864
Coordinates   2588664..2589773 (-) Length   369 a.a.
NCBI ID   WP_407346977.1    Uniprot ID   -
Organism   Congregibacter variabilis strain IMCC43200     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2583664..2594773
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R0135_RS11365 (R0135_11325) metF 2584122..2584958 (+) 837 WP_407346970.1 methylenetetrahydrofolate reductase [NAD(P)H] -
  R0135_RS11370 (R0135_11330) - 2584972..2585709 (+) 738 WP_407346971.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  R0135_RS11375 (R0135_11335) gshB 2585768..2586724 (+) 957 WP_407346973.1 glutathione synthase -
  R0135_RS11380 (R0135_11340) - 2586728..2587579 (+) 852 WP_407346974.1 TonB family protein -
  R0135_RS11385 (R0135_11345) - 2587698..2588219 (+) 522 WP_407349912.1 YqgE/AlgH family protein -
  R0135_RS11390 (R0135_11350) ruvX 2588221..2588649 (+) 429 WP_407346976.1 Holliday junction resolvase RuvX -
  R0135_RS11395 (R0135_11355) pilU 2588664..2589773 (-) 1110 WP_407346977.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  R0135_RS11400 (R0135_11360) pilT 2589827..2590861 (-) 1035 WP_407346978.1 type IV pilus twitching motility protein PilT Machinery gene
  R0135_RS11405 (R0135_11365) - 2590984..2591676 (+) 693 WP_407346979.1 YggS family pyridoxal phosphate-dependent enzyme -
  R0135_RS11410 (R0135_11370) proC 2591739..2592557 (+) 819 WP_407346980.1 pyrroline-5-carboxylate reductase -
  R0135_RS11415 (R0135_11375) - 2592601..2593188 (+) 588 WP_407346981.1 YggT family protein -
  R0135_RS11420 (R0135_11380) - 2593204..2594334 (+) 1131 WP_407346982.1 homoserine O-acetyltransferase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40498.20 Da        Isoelectric Point: 5.7664

>NTDB_id=893160 R0135_RS11395 WP_407346977.1 2588664..2589773(-) (pilU) [Congregibacter variabilis strain IMCC43200]
MKIDELFAKIVEAGASDGFVTARAAPSIKVDGEIRALSKESLSEEQARELVLATMSPSQQEDFMRDQECNYAVYSEDHGR
FRASAFVQRGQYGIVLRRIKDEIPKFGELGLPPIVAELAMAKRGLVIFVGGTGTGKSTSLAAMVGHRNRHSRGHILSIED
PIEFVHDHAGCLITQREVGIDTDSFETGLQNALRQAPDVILIGEVRSAETMAHAMSFAETGHLCLCTLHANNANQAIDRI
LSFFPVERHDQVLMDLSLNLNAMIAQQLLPKATGEGRVPVMEVLLNSPLISDHIRNGQVHLVKEVMAKSTEQGMQTLDQS
LLAKYCDGEITAEEAIRCADSANEVRLGIKMFDKRKSNYSAGVSLQVDR

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=893160 R0135_RS11395 WP_407346977.1 2588664..2589773(-) (pilU) [Congregibacter variabilis strain IMCC43200]
ATGAAAATTGACGAACTGTTCGCGAAGATCGTAGAGGCCGGTGCTTCTGATGGTTTTGTAACGGCTCGGGCCGCGCCATC
TATCAAGGTCGATGGAGAAATCCGCGCGCTGAGCAAGGAGAGCCTCAGCGAGGAGCAGGCCCGGGAACTGGTCTTGGCAA
CAATGTCCCCTTCGCAGCAAGAGGACTTTATGCGTGATCAGGAATGCAACTATGCCGTGTATTCCGAGGATCATGGGCGT
TTTCGGGCCAGTGCTTTTGTGCAGCGCGGGCAGTACGGTATTGTTTTACGCCGCATCAAAGATGAAATTCCGAAGTTTGG
TGAGCTGGGCCTACCACCGATTGTTGCGGAGCTTGCCATGGCCAAGCGCGGCCTGGTGATTTTTGTGGGTGGCACAGGAA
CGGGCAAATCCACCTCGCTGGCGGCAATGGTTGGGCACCGCAATCGCCACAGCCGAGGGCATATTCTGTCTATCGAAGAC
CCTATCGAGTTTGTCCATGACCACGCCGGCTGCCTGATCACCCAGCGTGAGGTGGGTATTGATACGGACTCTTTTGAAAC
AGGTCTGCAAAATGCGCTTCGGCAAGCCCCCGACGTCATTCTTATCGGCGAGGTGCGTTCTGCAGAGACCATGGCTCACG
CCATGAGCTTCGCGGAGACAGGCCATCTGTGTCTGTGCACATTGCACGCTAACAACGCCAACCAGGCTATTGACAGAATT
TTGAGTTTTTTCCCCGTGGAGAGACATGATCAGGTACTTATGGACCTGTCTTTGAACCTCAATGCCATGATCGCACAGCA
GTTGCTGCCCAAAGCCACGGGTGAGGGTCGCGTGCCGGTAATGGAGGTGTTGCTCAATTCGCCGCTTATCTCCGACCACA
TTCGTAACGGACAGGTGCATTTAGTCAAAGAAGTGATGGCTAAATCGACAGAACAGGGCATGCAAACTCTCGATCAATCG
CTGTTGGCCAAATACTGTGATGGTGAGATTACCGCTGAGGAGGCGATCCGTTGTGCTGATTCTGCTAACGAAGTGCGCCT
GGGTATTAAAATGTTCGACAAGCGCAAGAGCAATTACTCAGCTGGTGTAAGCCTGCAGGTCGATCGCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

57.143

98.645

0.564
  pilU Vibrio cholerae strain A1552

53.825

99.187

0.534
  pilU Acinetobacter baylyi ADP1

54.545

95.393

0.52
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.241

94.309

0.398
  pilT Acinetobacter baylyi ADP1

41.056

92.412

0.379
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.773

95.393

0.379
  pilT Vibrio cholerae strain A1552

39.773

95.393

0.379
  pilT Acinetobacter baumannii D1279779

40.896

90.786

0.371
  pilT Acinetobacter baumannii strain A118

40.896

90.786

0.371
  pilT Acinetobacter nosocomialis M2

40.896

90.786

0.371
  pilT Legionella pneumophila strain Lp02

40.533

91.599

0.371
  pilT Legionella pneumophila strain ERS1305867

40.533

91.599

0.371
  pilT Pseudomonas aeruginosa PAK

39.701

90.786

0.36