Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   R0134_RS02175 Genome accession   NZ_CP136856
Coordinates   472699..473736 (+) Length   345 a.a.
NCBI ID   WP_319783272.1    Uniprot ID   -
Organism   Oceanisphaera sp. IT1-181     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 467699..478736
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R0134_RS02140 hemW 467814..468959 (-) 1146 WP_319783267.1 radical SAM family heme chaperone HemW -
  R0134_RS02145 rdgB 468959..469645 (-) 687 WP_319783268.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  R0134_RS02150 - 469757..470179 (-) 423 WP_319783269.1 DUF4426 domain-containing protein -
  R0134_RS02155 - 470219..470515 (-) 297 WP_319784284.1 DUF167 family protein -
  R0134_RS02160 - 470518..471078 (-) 561 WP_319783270.1 YggT family protein -
  R0134_RS02165 proC 471088..471909 (-) 822 WP_319784285.1 pyrroline-5-carboxylate reductase -
  R0134_RS02170 - 471971..472663 (-) 693 WP_319783271.1 YggS family pyridoxal phosphate-dependent enzyme -
  R0134_RS02175 pilT 472699..473736 (+) 1038 WP_319783272.1 type IV pilus twitching motility protein PilT Machinery gene
  R0134_RS02180 pilU 473749..474858 (+) 1110 WP_319783273.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  R0134_RS02185 yaaA 474983..475756 (+) 774 WP_319783274.1 peroxide stress protein YaaA -
  R0134_RS02190 - 476313..476465 (-) 153 WP_319783275.1 hypothetical protein -
  R0134_RS02195 - 476671..477996 (+) 1326 WP_319782802.1 IS4 family transposase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38285.85 Da        Isoelectric Point: 6.7414

>NTDB_id=893116 R0134_RS02175 WP_319783272.1 472699..473736(+) (pilT) [Oceanisphaera sp. IT1-181]
MDITELLAFSVKHNASDLHLSAGVPPQIRVDGEVRKINLPILEHTEVHRLVYEIMNDRQRQEFEDNLEVDFSFELPGLAR
FRVNAYHQARGAAAVFRVIPSEVLSLEQLGGPEIFRRIAEYQRGLVLVTGPTGSGKSTTLAAMVDHINDNYNKHILTIED
PIEFVHSNKKCLINQREVHRDTHSFSNALRSSLREDPDIILVGELRDLETIRLALTAAETGHLVFATLHTSSAAKTIDRI
VDVFPGAEKAMVRSMLSESLRAVISQTLLKKPNGGRIAAHEIMLGTPAIRNLIREDKVAQMYSVIQTGLAHGMQTMDQCL
KQLVNGGLISAVDARAKATDPQSIQ

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=893116 R0134_RS02175 WP_319783272.1 472699..473736(+) (pilT) [Oceanisphaera sp. IT1-181]
ATGGATATTACGGAACTATTGGCGTTTAGTGTAAAGCATAATGCCTCGGATCTGCACCTGTCTGCGGGCGTACCGCCACA
AATCAGAGTGGACGGTGAAGTGCGCAAGATTAACTTGCCGATATTAGAGCATACCGAAGTGCACCGTTTGGTGTACGAGA
TTATGAATGATCGCCAGCGTCAAGAGTTTGAAGACAACCTCGAAGTCGACTTCTCGTTTGAACTGCCAGGCTTGGCTCGC
TTTCGTGTTAACGCCTATCACCAAGCACGCGGGGCGGCCGCCGTATTCCGAGTAATTCCCAGCGAAGTGCTGAGCTTAGA
ACAACTGGGCGGACCGGAAATCTTTCGGCGCATTGCCGAGTATCAAAGAGGCTTAGTGCTGGTCACAGGTCCTACTGGTT
CGGGTAAATCCACCACCCTCGCGGCCATGGTGGATCATATAAATGACAACTATAATAAGCACATTCTGACCATCGAAGAT
CCCATTGAATTTGTGCACAGTAATAAAAAATGCCTGATCAACCAGCGGGAAGTACACAGAGACACCCACTCCTTTAGTAA
CGCACTGCGCTCCTCGTTACGAGAAGATCCGGATATTATTTTGGTGGGCGAGCTGCGCGACTTAGAAACTATTCGCTTGG
CACTCACCGCCGCCGAAACCGGCCATTTAGTGTTTGCGACCTTGCATACCTCGTCCGCCGCCAAAACCATAGACCGTATT
GTGGATGTGTTTCCGGGCGCGGAAAAAGCCATGGTGCGCTCCATGCTATCTGAGTCACTGCGTGCGGTGATTTCTCAAAC
CTTGCTGAAAAAGCCCAATGGCGGACGCATCGCGGCTCATGAGATCATGCTCGGCACGCCGGCCATTCGTAACTTGATCC
GCGAAGATAAAGTCGCCCAGATGTATTCGGTGATCCAAACCGGTCTGGCGCACGGCATGCAAACCATGGATCAATGTTTG
AAGCAGCTGGTGAACGGTGGTTTGATCTCAGCGGTGGATGCGCGAGCTAAAGCCACAGATCCTCAGAGTATTCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

75.291

99.71

0.751
  pilT Acinetobacter baumannii strain A118

75.291

99.71

0.751
  pilT Pseudomonas aeruginosa PAK

75.291

99.71

0.751
  pilT Acinetobacter nosocomialis M2

75.291

99.71

0.751
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

76.261

97.681

0.745
  pilT Vibrio cholerae strain A1552

76.261

97.681

0.745
  pilT Acinetobacter baylyi ADP1

74.709

99.71

0.745
  pilT Pseudomonas stutzeri DSM 10701

74.419

99.71

0.742
  pilT Legionella pneumophila strain Lp02

73.03

95.652

0.699
  pilT Legionella pneumophila strain ERS1305867

73.03

95.652

0.699
  pilT Neisseria meningitidis 8013

66.957

100

0.67
  pilT Neisseria gonorrhoeae MS11

66.667

100

0.667
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.603

99.42

0.513
  pilU Vibrio cholerae strain A1552

42.388

97.101

0.412
  pilU Pseudomonas stutzeri DSM 10701

41.194

97.101

0.4
  pilU Acinetobacter baylyi ADP1

39.769

100

0.4