Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   R0134_RS02180 Genome accession   NZ_CP136856
Coordinates   473749..474858 (+) Length   369 a.a.
NCBI ID   WP_319783273.1    Uniprot ID   -
Organism   Oceanisphaera sp. IT1-181     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 468749..479858
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R0134_RS02145 rdgB 468959..469645 (-) 687 WP_319783268.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  R0134_RS02150 - 469757..470179 (-) 423 WP_319783269.1 DUF4426 domain-containing protein -
  R0134_RS02155 - 470219..470515 (-) 297 WP_319784284.1 DUF167 family protein -
  R0134_RS02160 - 470518..471078 (-) 561 WP_319783270.1 YggT family protein -
  R0134_RS02165 proC 471088..471909 (-) 822 WP_319784285.1 pyrroline-5-carboxylate reductase -
  R0134_RS02170 - 471971..472663 (-) 693 WP_319783271.1 YggS family pyridoxal phosphate-dependent enzyme -
  R0134_RS02175 pilT 472699..473736 (+) 1038 WP_319783272.1 type IV pilus twitching motility protein PilT Machinery gene
  R0134_RS02180 pilU 473749..474858 (+) 1110 WP_319783273.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  R0134_RS02185 yaaA 474983..475756 (+) 774 WP_319783274.1 peroxide stress protein YaaA -
  R0134_RS02190 - 476313..476465 (-) 153 WP_319783275.1 hypothetical protein -
  R0134_RS02195 - 476671..477996 (+) 1326 WP_319782802.1 IS4 family transposase -
  R0134_RS02200 srmB 478072..479433 (-) 1362 WP_319783276.1 ATP-dependent RNA helicase SrmB -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41302.11 Da        Isoelectric Point: 6.1476

>NTDB_id=893117 R0134_RS02180 WP_319783273.1 473749..474858(+) (pilU) [Oceanisphaera sp. IT1-181]
MQLTSFLQAMVEQQASDLYISVGVPAMIKSHGNLVAISDTKLTAADALALVESCMTPEWHMRFHQQKEANFAISDEQHGR
FRVSAFWQQEKAGMVIRRIESRIPSFEELYLPDSLREVAMAKRGLVLFVGGTGTGKSTTQAAMIGHRNRHADDHILTIED
PIEFVHQHERSIITQREVGIDTESFEAALKSSLRQAPNVILIGEIRSEETMGYALAFAETGHLCMATLHANNANQALDRI
MHLVPESKHRQLQFDLSFNLRAIVAQQLIPTREGDKRRAAFEVLLNTPLMADLIRKGELHRLKEVMSKSREQGMQTFDQA
LFDLYNQNQIGYSEALAYADSANDLRLMIKLQGGGTGLDAGLMDNVTIE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=893117 R0134_RS02180 WP_319783273.1 473749..474858(+) (pilU) [Oceanisphaera sp. IT1-181]
ATGCAGTTAACCTCTTTTTTGCAGGCCATGGTGGAGCAACAAGCGTCAGACTTGTATATCTCGGTGGGCGTGCCTGCCAT
GATCAAAAGCCACGGCAATTTAGTTGCTATTAGTGATACTAAACTGACCGCCGCCGATGCCTTGGCGCTGGTGGAGTCGT
GCATGACGCCGGAGTGGCACATGCGTTTTCATCAGCAGAAAGAAGCCAACTTCGCCATTAGTGATGAGCAGCATGGCCGT
TTTCGGGTCAGTGCTTTTTGGCAGCAAGAGAAAGCCGGAATGGTGATCCGGCGCATTGAAAGCAGGATCCCCTCCTTTGA
AGAGCTGTATTTGCCCGATAGCCTGCGCGAAGTGGCCATGGCCAAACGCGGTTTAGTACTGTTTGTAGGCGGCACGGGTA
CCGGTAAGTCGACCACGCAAGCTGCCATGATTGGGCATCGTAATCGCCATGCAGACGATCATATTTTGACCATCGAAGAT
CCGATTGAATTTGTGCATCAGCATGAGCGTTCTATTATCACCCAGCGGGAAGTGGGCATAGATACTGAATCCTTTGAAGC
CGCACTCAAAAGCTCCTTGCGCCAAGCGCCGAACGTGATTTTAATCGGTGAAATTCGCTCCGAAGAAACCATGGGCTATG
CGCTGGCCTTTGCTGAAACCGGTCATTTGTGTATGGCGACTTTGCACGCTAATAACGCCAACCAAGCACTGGATCGCATT
ATGCATTTAGTGCCTGAAAGTAAGCATCGGCAGTTACAGTTTGATTTATCTTTTAACCTGCGCGCAATCGTGGCCCAGCA
GCTGATTCCCACTCGTGAAGGCGACAAGCGTCGTGCGGCCTTTGAGGTGCTGCTTAATACGCCGTTAATGGCGGATTTGA
TCCGCAAAGGTGAGCTGCACCGTTTGAAAGAGGTAATGAGCAAGTCTCGTGAACAGGGTATGCAGACCTTCGATCAAGCC
TTATTTGATCTCTATAACCAGAATCAGATCGGTTACAGCGAAGCCCTGGCTTATGCAGACTCAGCCAACGACTTGCGCTT
GATGATCAAGTTGCAAGGCGGCGGCACAGGACTGGACGCGGGCTTGATGGATAACGTGACCATAGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

58.808

100

0.588
  pilU Pseudomonas stutzeri DSM 10701

59.544

95.122

0.566
  pilU Acinetobacter baylyi ADP1

57.955

95.393

0.553
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.888

91.87

0.385
  pilT Neisseria gonorrhoeae MS11

40.597

90.786

0.369
  pilT Neisseria meningitidis 8013

40.785

89.702

0.366
  pilT Legionella pneumophila strain Lp02

40.785

89.702

0.366
  pilT Legionella pneumophila strain ERS1305867

40.785

89.702

0.366
  pilT Acinetobacter baylyi ADP1

39.003

92.412

0.36