Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OCU56_RS10855 Genome accession   NZ_AP024900
Coordinates   2389613..2390659 (-) Length   348 a.a.
NCBI ID   WP_261873235.1    Uniprot ID   -
Organism   Vibrio rarus strain LMG 23674     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2384613..2395659
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCU56_RS10825 - 2384632..2385327 (+) 696 WP_261873229.1 endonuclease -
  OCU56_RS10830 rsmE 2385422..2386153 (+) 732 WP_261873230.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OCU56_RS10835 gshB 2386163..2387131 (+) 969 WP_261873231.1 glutathione synthase -
  OCU56_RS10840 - 2387272..2387832 (+) 561 WP_261873232.1 YqgE/AlgH family protein -
  OCU56_RS10845 ruvX 2387829..2388254 (+) 426 WP_261873233.1 Holliday junction resolvase RuvX -
  OCU56_RS10850 pilU 2388263..2389366 (-) 1104 WP_261873234.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCU56_RS10855 pilT 2389613..2390659 (-) 1047 WP_261873235.1 type IV pilus twitching motility protein PilT Machinery gene
  OCU56_RS10860 - 2390684..2391394 (+) 711 WP_261873236.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCU56_RS10865 proC 2391418..2392242 (+) 825 WP_261873237.1 pyrroline-5-carboxylate reductase -
  OCU56_RS10870 - 2392261..2392830 (+) 570 WP_261873238.1 YggT family protein -
  OCU56_RS10875 - 2392862..2393305 (+) 444 WP_261873239.1 DUF4426 domain-containing protein -
  OCU56_RS10880 - 2393312..2393926 (+) 615 WP_261873240.1 XTP/dITP diphosphatase -
  OCU56_RS10885 hemW 2393923..2395095 (+) 1173 WP_261873241.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38686.10 Da        Isoelectric Point: 5.7867

>NTDB_id=89291 OCU56_RS10855 WP_261873235.1 2389613..2390659(-) (pilT) [Vibrio rarus strain LMG 23674]
MDITELLRFSVKHNASDLHLSSGLPPMVRIDGDVRRLEMAEFSESEVHDLIFDIMDDAQRSEFESKLEIDFSIELTGVGR
FRVNAFNQSRGASAVFRTIPTIIPTLEQLDCPPTFEQIVNYEKGLVLVTGPTGSGKSTTLAAMVDYINRNHNKHILTIED
PIEFVHNSAKSLINQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFATLHTSSAAKSIDRI
IDVFPAGDKNMVRSMLSESLRAVIAQKLLKRIGGGRVACHEIMLANPAIRNLIREDKVAQMYSIIQTGAAQGMRTMEQSV
NQLMQQNVVTREEAEAKIELQSSFGSSF

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=89291 OCU56_RS10855 WP_261873235.1 2389613..2390659(-) (pilT) [Vibrio rarus strain LMG 23674]
ATGGATATCACCGAATTGCTTCGCTTTAGTGTAAAGCATAATGCATCAGATCTACATCTTTCATCGGGTTTGCCACCTAT
GGTGAGAATTGATGGTGATGTGCGTCGCTTAGAGATGGCGGAATTTAGTGAATCAGAGGTGCATGATCTCATTTTTGACA
TAATGGATGATGCGCAGCGAAGTGAGTTTGAGTCAAAGTTAGAGATTGATTTTTCTATCGAGCTCACAGGTGTAGGGCGT
TTTAGGGTTAACGCTTTTAATCAAAGCCGTGGTGCTTCGGCGGTATTTCGTACCATCCCTACTATTATTCCAACCTTAGA
ACAATTAGATTGCCCGCCGACCTTTGAACAGATTGTTAATTATGAAAAAGGCTTAGTGCTAGTCACCGGACCGACAGGCT
CAGGTAAGTCCACCACTTTGGCGGCAATGGTGGATTATATTAACCGTAACCACAATAAGCATATATTAACCATTGAAGAC
CCTATTGAGTTTGTACATAACAGCGCTAAATCTTTGATTAATCAGCGTGAAGTGCATCGTGATACTCATAGTTTTAAAGC
GGCACTGCGCTCTGCTTTACGTGAAGATCCCGATGTGATCTTGGTGGGGGAGTTACGGGATCAAGAAACCATAAGTTTGG
CGCTTACCGCCGCGGAAACAGGGCATCTAGTGTTTGCTACTTTGCATACCAGTTCTGCGGCAAAGAGCATAGACCGAATC
ATTGATGTGTTTCCGGCTGGAGATAAAAACATGGTGCGCTCTATGCTTTCCGAATCTTTACGTGCGGTAATTGCCCAGAA
ATTATTGAAGCGTATTGGTGGTGGCCGAGTGGCGTGCCACGAAATTATGTTAGCCAACCCTGCCATTCGTAACTTGATTC
GAGAAGATAAAGTGGCGCAGATGTATTCGATTATTCAAACGGGTGCCGCTCAAGGTATGCGAACCATGGAACAGTCGGTG
AATCAGTTAATGCAACAAAATGTGGTAACAAGAGAAGAGGCGGAAGCGAAAATAGAATTGCAAAGCAGTTTTGGTTCAAG
TTTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

81.471

97.701

0.796
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

81.471

97.701

0.796
  pilT Acinetobacter baylyi ADP1

67.442

98.851

0.667
  pilT Acinetobacter baumannii D1279779

67.151

98.851

0.664
  pilT Acinetobacter baumannii strain A118

67.151

98.851

0.664
  pilT Acinetobacter nosocomialis M2

67.151

98.851

0.664
  pilT Pseudomonas stutzeri DSM 10701

66.86

98.851

0.661
  pilT Pseudomonas aeruginosa PAK

66.279

98.851

0.655
  pilT Neisseria meningitidis 8013

62.464

100

0.626
  pilT Legionella pneumophila strain Lp02

64.97

95.977

0.624
  pilT Legionella pneumophila strain ERS1305867

64.97

95.977

0.624
  pilT Neisseria gonorrhoeae MS11

63.582

96.264

0.612
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.642

96.264

0.497
  pilU Pseudomonas stutzeri DSM 10701

41.194

96.264

0.397
  pilU Vibrio cholerae strain A1552

40.896

96.264

0.394
  pilU Acinetobacter baylyi ADP1

37.798

96.552

0.365

Multiple sequence alignment