Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OCU56_RS10850 Genome accession   NZ_AP024900
Coordinates   2388263..2389366 (-) Length   367 a.a.
NCBI ID   WP_261873234.1    Uniprot ID   -
Organism   Vibrio rarus strain LMG 23674     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2383263..2394366
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCU56_RS10820 - 2384015..2384509 (+) 495 WP_261873228.1 SprT family zinc-dependent metalloprotease -
  OCU56_RS10825 - 2384632..2385327 (+) 696 WP_261873229.1 endonuclease -
  OCU56_RS10830 rsmE 2385422..2386153 (+) 732 WP_261873230.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OCU56_RS10835 gshB 2386163..2387131 (+) 969 WP_261873231.1 glutathione synthase -
  OCU56_RS10840 - 2387272..2387832 (+) 561 WP_261873232.1 YqgE/AlgH family protein -
  OCU56_RS10845 ruvX 2387829..2388254 (+) 426 WP_261873233.1 Holliday junction resolvase RuvX -
  OCU56_RS10850 pilU 2388263..2389366 (-) 1104 WP_261873234.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCU56_RS10855 pilT 2389613..2390659 (-) 1047 WP_261873235.1 type IV pilus twitching motility protein PilT Machinery gene
  OCU56_RS10860 - 2390684..2391394 (+) 711 WP_261873236.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCU56_RS10865 proC 2391418..2392242 (+) 825 WP_261873237.1 pyrroline-5-carboxylate reductase -
  OCU56_RS10870 - 2392261..2392830 (+) 570 WP_261873238.1 YggT family protein -
  OCU56_RS10875 - 2392862..2393305 (+) 444 WP_261873239.1 DUF4426 domain-containing protein -
  OCU56_RS10880 - 2393312..2393926 (+) 615 WP_261873240.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 367 a.a.        Molecular weight: 40977.89 Da        Isoelectric Point: 6.5667

>NTDB_id=89290 OCU56_RS10850 WP_261873234.1 2388263..2389366(-) (pilU) [Vibrio rarus strain LMG 23674]
MELKELLEAMTQHRASDLYITVDSPCMFRIDGELQPFADKLHQSGVTALLDSIMDNDRRKEYRAHREANFAIESESGRFR
VSAFFQRDLPGAVIRHIETKIPSFAELGLPDTLKDLAMVKRGLVLVVGATGSGKSTTLAAMVGHRNNTQRGHILTVEDPI
EFVHQHQQSIVTQREVGLDTESYEVALKNSLRQAPDMIVIGEIRSAETMRYAMTFAETGHLCVATLHANNANQALERILH
LVPREQREHFLLDLSLNLRGIVGQQLLKATQGTGRHVALEILQVTPRAADLIRQGEITEIKNYMAKTKQVGICTFDQSLF
ELLKQGKISEQDALRSADSANDLRLMLKNFKGVSSDGPLDEIKIDMG

Nucleotide


Download         Length: 1104 bp        

>NTDB_id=89290 OCU56_RS10850 WP_261873234.1 2388263..2389366(-) (pilU) [Vibrio rarus strain LMG 23674]
ATGGAACTAAAAGAGTTGTTAGAAGCGATGACGCAACATAGAGCGTCTGATCTTTATATTACGGTAGATTCTCCTTGTAT
GTTTCGCATTGATGGTGAGCTGCAACCTTTCGCGGATAAACTCCATCAGTCCGGTGTTACCGCACTGCTTGATAGCATAA
TGGATAACGATAGGCGTAAAGAGTATCGCGCCCATAGGGAAGCAAATTTTGCTATTGAAAGTGAGTCGGGGCGTTTTCGG
GTCAGTGCTTTTTTCCAGCGTGATTTACCGGGTGCAGTGATTCGTCATATCGAAACAAAAATTCCTTCTTTTGCTGAGCT
TGGTTTACCGGATACCTTAAAAGATTTGGCGATGGTGAAACGAGGTTTAGTGCTGGTGGTTGGGGCGACGGGTTCTGGCA
AGTCTACGACTCTTGCCGCTATGGTGGGGCATCGAAATAACACCCAACGTGGCCATATTTTGACCGTGGAAGATCCGATA
GAGTTTGTTCATCAGCATCAACAAAGCATAGTGACTCAGCGTGAAGTTGGTTTGGACACTGAAAGTTATGAAGTGGCGCT
AAAAAACTCTCTTCGCCAAGCCCCTGATATGATAGTGATTGGTGAGATACGTTCAGCGGAAACCATGCGCTATGCTATGA
CGTTTGCTGAAACGGGGCACTTGTGTGTGGCCACTTTGCACGCCAACAATGCCAATCAGGCTCTAGAGCGAATTTTACAT
TTAGTGCCAAGAGAACAAAGAGAGCACTTTTTGTTGGATCTTTCCCTTAATTTAAGAGGAATAGTGGGTCAGCAATTGCT
TAAAGCAACACAAGGTACGGGCCGACATGTAGCGTTAGAGATTTTGCAGGTGACGCCAAGGGCGGCGGATCTTATTCGCC
AAGGAGAGATCACCGAGATCAAGAACTACATGGCTAAGACAAAGCAGGTGGGGATTTGTACTTTTGATCAGTCTTTATTT
GAGTTACTTAAACAGGGCAAGATCAGCGAACAAGACGCATTAAGAAGCGCAGATTCGGCCAACGATCTGCGCTTGATGTT
AAAAAACTTTAAAGGCGTGAGCAGTGATGGCCCACTAGATGAGATTAAAATTGATATGGGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

68.12

100

0.681
  pilU Pseudomonas stutzeri DSM 10701

54.039

97.82

0.529
  pilU Acinetobacter baylyi ADP1

52.893

98.91

0.523
  pilT Legionella pneumophila strain Lp02

43.363

92.371

0.401
  pilT Legionella pneumophila strain ERS1305867

43.363

92.371

0.401
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.765

92.643

0.387
  pilT Acinetobacter baylyi ADP1

41.642

92.916

0.387
  pilT Pseudomonas stutzeri DSM 10701

40.936

93.188

0.381
  pilT Acinetobacter baumannii D1279779

41.003

92.371

0.379
  pilT Acinetobacter baumannii strain A118

41.003

92.371

0.379
  pilT Acinetobacter nosocomialis M2

41.003

92.371

0.379
  pilT Pseudomonas aeruginosa PAK

40.708

92.371

0.376
  pilT Vibrio cholerae strain A1552

40.719

91.008

0.371
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.719

91.008

0.371
  pilT Neisseria gonorrhoeae MS11

39.42

94.005

0.371
  pilT Neisseria meningitidis 8013

39.42

94.005

0.371

Multiple sequence alignment