Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   R1Z03_RS20420 Genome accession   NZ_CP136797
Coordinates   4435537..4436580 (+) Length   347 a.a.
NCBI ID   WP_092744851.1    Uniprot ID   -
Organism   Acidovorax sp. BLS4     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4430537..4441580
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R1Z03_RS20405 (R1Z03_20405) - 4431445..4432836 (-) 1392 WP_317354525.1 hypothetical protein -
  R1Z03_RS20410 (R1Z03_20410) - 4433145..4434755 (+) 1611 WP_317354528.1 methyl-accepting chemotaxis protein -
  R1Z03_RS20415 (R1Z03_20415) - 4434775..4435497 (-) 723 WP_317354530.1 YggS family pyridoxal phosphate-dependent enzyme -
  R1Z03_RS20420 (R1Z03_20420) pilT 4435537..4436580 (+) 1044 WP_092744851.1 type IV pilus twitching motility protein PilT Machinery gene
  R1Z03_RS20425 (R1Z03_20425) - 4436658..4437293 (+) 636 WP_272566930.1 cyclic nucleotide-binding domain-containing protein -
  R1Z03_RS20430 (R1Z03_20430) pilU 4437327..4438463 (+) 1137 WP_272566929.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  R1Z03_RS20435 (R1Z03_20435) - 4438531..4439463 (+) 933 WP_284431083.1 NAD(P)-dependent oxidoreductase -
  R1Z03_RS20440 (R1Z03_20440) - 4439566..4440243 (-) 678 WP_372588178.1 BON domain-containing protein -
  R1Z03_RS20445 (R1Z03_20445) - 4440240..4440836 (-) 597 WP_284431081.1 SIS domain-containing protein -
  R1Z03_RS20450 (R1Z03_20450) - 4440940..4441323 (-) 384 WP_284431080.1 YraN family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38030.79 Da        Isoelectric Point: 6.7744

>NTDB_id=892673 R1Z03_RS20420 WP_092744851.1 4435537..4436580(+) (pilT) [Acidovorax sp. BLS4]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDALDHKTVHAMVYDIMSDAQRKAYEEFLEVDFSFEIEGLAR
FRVNAFNQNRGAAAVFRTIPSKILTLEQLNAPKIFGELALKPRGLVLVTGPTGSGKSTTLAAMVNFLNESEYGHILTVED
PIEFVHESKKCLINQREVGPMTLSFAAALKSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLQAVISQTLCKTKDGQGRVAAHEIMLGTSAIRNLIREAKVAQMYSTIQTSSSAGMQTLDQN
LTDLVRRNVISPAEARGKAKIPDNFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=892673 R1Z03_RS20420 WP_092744851.1 4435537..4436580(+) (pilT) [Acidovorax sp. BLS4]
GTGGACATCACCCAACTGCTGGCATTCAGCGTGAAGAACAAGGCTTCTGACCTGCACTTGTCGGCGGGCCTTCCACCCAT
GATCCGGGTCCACGGCGACGTGCGGCGCATCAACGTCGATGCGCTCGACCACAAGACCGTCCACGCCATGGTGTACGACA
TCATGAGCGATGCGCAGCGCAAGGCGTACGAAGAATTCCTGGAAGTGGACTTTTCCTTCGAGATCGAGGGCCTGGCGCGC
TTTCGCGTCAACGCGTTCAATCAGAACCGCGGCGCGGCCGCTGTGTTCCGGACCATTCCGAGCAAGATCCTGACGCTGGA
GCAGCTCAACGCCCCCAAGATCTTCGGCGAACTGGCCTTGAAACCGCGCGGCCTGGTGCTGGTCACGGGCCCCACGGGCT
CGGGCAAGTCCACCACGCTGGCGGCCATGGTCAACTTCCTGAACGAAAGCGAATACGGCCACATCCTCACGGTGGAAGAC
CCGATCGAGTTCGTGCACGAATCCAAGAAGTGCCTGATCAACCAGCGCGAGGTCGGGCCGATGACGCTGTCGTTCGCGGC
CGCCCTCAAGTCGGCCCTGCGCGAAGATCCGGACGCCATCCTGGTGGGCGAAATGCGCGACCTGGAAACCATCCGCCTGG
CCATGACGGCCGCGGAAACGGGCCACTTGGTGTTCGGCACGCTGCACACCTCCAGCGCCGCCAAGACCATCGACCGGATC
ATCGACGTGTTCCCCGCCGAGGAAAAGGAAATGGTGCGGGCCATGCTGTCCGAGTCGCTGCAGGCCGTCATTTCGCAGAC
GCTGTGCAAGACCAAGGACGGCCAGGGCCGTGTCGCGGCGCACGAGATCATGCTGGGCACCAGCGCCATCCGCAACCTGA
TCCGCGAGGCCAAGGTGGCACAGATGTACTCCACCATCCAGACCAGCAGCAGCGCCGGCATGCAGACCCTCGACCAGAAC
CTGACCGACCTCGTCCGGCGCAACGTCATCAGCCCGGCCGAGGCGCGGGGCAAGGCGAAGATCCCCGACAACTTCCCGGG
TTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

75.942

99.424

0.755
  pilT Acinetobacter nosocomialis M2

75.942

99.424

0.755
  pilT Acinetobacter baumannii D1279779

75.942

99.424

0.755
  pilT Acinetobacter baumannii strain A118

75.942

99.424

0.755
  pilT Pseudomonas stutzeri DSM 10701

75.362

99.424

0.749
  pilT Acinetobacter baylyi ADP1

74.203

99.424

0.738
  pilT Legionella pneumophila strain ERS1305867

73.746

97.695

0.72
  pilT Legionella pneumophila strain Lp02

73.746

97.695

0.72
  pilT Neisseria gonorrhoeae MS11

68.235

97.983

0.669
  pilT Neisseria meningitidis 8013

68.235

97.983

0.669
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.449

98.271

0.663
  pilT Vibrio cholerae strain A1552

67.449

98.271

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

99.424

0.496
  pilU Vibrio cholerae strain A1552

43.881

96.542

0.424
  pilU Acinetobacter baylyi ADP1

41.908

99.712

0.418
  pilU Pseudomonas stutzeri DSM 10701

41.493

96.542

0.401