Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   R1Z03_RS20430 Genome accession   NZ_CP136797
Coordinates   4437327..4438463 (+) Length   378 a.a.
NCBI ID   WP_272566929.1    Uniprot ID   -
Organism   Acidovorax sp. BLS4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4432327..4443463
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R1Z03_RS20410 (R1Z03_20410) - 4433145..4434755 (+) 1611 WP_317354528.1 methyl-accepting chemotaxis protein -
  R1Z03_RS20415 (R1Z03_20415) - 4434775..4435497 (-) 723 WP_317354530.1 YggS family pyridoxal phosphate-dependent enzyme -
  R1Z03_RS20420 (R1Z03_20420) pilT 4435537..4436580 (+) 1044 WP_092744851.1 type IV pilus twitching motility protein PilT Machinery gene
  R1Z03_RS20425 (R1Z03_20425) - 4436658..4437293 (+) 636 WP_272566930.1 cyclic nucleotide-binding domain-containing protein -
  R1Z03_RS20430 (R1Z03_20430) pilU 4437327..4438463 (+) 1137 WP_272566929.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  R1Z03_RS20435 (R1Z03_20435) - 4438531..4439463 (+) 933 WP_284431083.1 NAD(P)-dependent oxidoreductase -
  R1Z03_RS20440 (R1Z03_20440) - 4439566..4440243 (-) 678 WP_372588178.1 BON domain-containing protein -
  R1Z03_RS20445 (R1Z03_20445) - 4440240..4440836 (-) 597 WP_284431081.1 SIS domain-containing protein -
  R1Z03_RS20450 (R1Z03_20450) - 4440940..4441323 (-) 384 WP_284431080.1 YraN family protein -
  R1Z03_RS20455 (R1Z03_20455) rsmI 4441323..4442243 (+) 921 WP_317354539.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41776.26 Da        Isoelectric Point: 6.9080

>NTDB_id=892674 R1Z03_RS20430 WP_272566929.1 4437327..4438463(+) (pilU) [Acidovorax sp. BLS4]
MERDQASKFINDLLKLMVSRGGSDLFITAEFPPAIKVDGKVTKVSPQPLTPAHTLTLARAIMSDKQVADFERTKECNFAI
SPAGIGRFRVNAFIQQSRVGMVLRTIPLTLPTIDGLGVPQVLKEVTMTKRGLCILVGATGSGKSTTLAAMVDWRNENSFG
HIITVEDPIEFVHPHKNCVVTQREVGLDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCLATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRGMISQRLIPKQDGKGRAAAVEVMLNTPLIADLIFKGEVAEIKEIMKKSRNLG
MQTFDQSLFDLFEANVITYEDALRNADSLNDLRLQIKLGSQRAKTTDLASGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=892674 R1Z03_RS20430 WP_272566929.1 4437327..4438463(+) (pilU) [Acidovorax sp. BLS4]
ATGGAACGCGATCAGGCCAGTAAATTCATCAACGATCTGCTCAAGCTCATGGTGAGCCGGGGGGGCAGCGACCTGTTCAT
CACGGCCGAGTTTCCGCCCGCGATCAAGGTGGACGGCAAGGTCACCAAGGTGTCCCCCCAGCCGCTCACTCCCGCCCACA
CGCTGACGCTGGCGCGCGCCATCATGAGCGACAAGCAGGTGGCCGACTTCGAGCGCACGAAGGAGTGCAACTTCGCCATC
TCGCCCGCCGGCATCGGGCGCTTTCGCGTGAACGCGTTCATCCAGCAAAGCCGCGTCGGCATGGTGCTGCGGACCATTCC
GCTCACGCTGCCGACCATCGACGGGCTCGGCGTGCCGCAGGTGCTCAAGGAAGTCACGATGACCAAGCGGGGCCTGTGCA
TTCTGGTGGGCGCCACGGGCTCGGGCAAATCGACCACGCTGGCGGCAATGGTGGACTGGCGCAACGAGAACTCGTTCGGC
CACATCATCACCGTGGAAGACCCCATCGAGTTCGTGCATCCGCACAAGAACTGCGTCGTCACCCAGCGCGAAGTGGGACT
CGATACCGACAGCTGGGAAGCCGCCCTCAAGAACACGCTTCGCCAGGCCCCGGACGTCATCCTGATGGGCGAGATCCGCG
ACCGCGAAACCATGGAGCATGCGGTCGCCTTCGCCGAAACAGGCCACCTGTGCCTGGCCACACTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGCATCATCAATTTCTTTCCGGAAGAGCGGCGCGCGCAACTCCTCATGGACCTGTCGCTCAACCT
GCGCGGCATGATCTCCCAGCGCCTCATCCCCAAGCAGGATGGCAAGGGCCGGGCCGCGGCGGTCGAGGTGATGCTGAACA
CGCCCCTGATCGCCGACCTGATCTTCAAGGGCGAAGTCGCCGAGATCAAGGAGATCATGAAAAAGAGCCGGAACCTCGGC
ATGCAGACCTTCGACCAATCGCTGTTCGACCTGTTCGAGGCCAACGTCATCACCTACGAAGACGCGCTGCGCAATGCCGA
CTCACTGAACGACCTGCGTTTGCAGATCAAGCTGGGCAGCCAGCGCGCCAAGACCACCGACCTGGCCTCGGGCACCGAGC
ACTTCGCCATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.756

92.328

0.598
  pilU Acinetobacter baylyi ADP1

57.542

94.709

0.545
  pilU Vibrio cholerae strain A1552

53.444

96.032

0.513
  pilT Pseudomonas aeruginosa PAK

45.697

89.153

0.407
  pilT Acinetobacter baumannii strain A118

45.045

88.095

0.397
  pilT Acinetobacter nosocomialis M2

45.045

88.095

0.397
  pilT Acinetobacter baumannii D1279779

45.045

88.095

0.397
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.214

89.153

0.394
  pilT Pseudomonas stutzeri DSM 10701

43.62

89.153

0.389
  pilT Legionella pneumophila strain ERS1305867

43.844

88.095

0.386
  pilT Legionella pneumophila strain Lp02

43.844

88.095

0.386
  pilT Acinetobacter baylyi ADP1

43.598

86.772

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.169

87.831

0.37
  pilT Vibrio cholerae strain A1552

42.169

87.831

0.37