Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   R1T43_RS05300 Genome accession   NZ_CP136708
Coordinates   1248291..1249334 (+) Length   347 a.a.
NCBI ID   WP_317353626.1    Uniprot ID   A0AAU0H4R9
Organism   Alteromonas sp. CI.11.F.A3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1243291..1254334
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R1T43_RS05275 (R1T43_05275) - 1244548..1245402 (-) 855 WP_410549000.1 transposase -
  R1T43_RS05280 (R1T43_05280) - 1245679..1246119 (-) 441 WP_317353617.1 DUF4426 domain-containing protein -
  R1T43_RS05285 (R1T43_05285) - 1246142..1246681 (-) 540 WP_013782901.1 YggT family protein -
  R1T43_RS05290 (R1T43_05290) proC 1246703..1247524 (-) 822 WP_317353622.1 pyrroline-5-carboxylate reductase -
  R1T43_RS05295 (R1T43_05295) - 1247580..1248266 (-) 687 WP_317353623.1 YggS family pyridoxal phosphate-dependent enzyme -
  R1T43_RS05300 (R1T43_05300) pilT 1248291..1249334 (+) 1044 WP_317353626.1 type IV pilus twitching motility protein PilT Machinery gene
  R1T43_RS05305 (R1T43_05305) pilU 1249344..1250456 (+) 1113 WP_317353627.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  R1T43_RS05310 (R1T43_05310) - 1250462..1250776 (+) 315 WP_317353629.1 DUF2007 domain-containing protein -
  R1T43_RS05315 (R1T43_05315) ruvX 1251131..1251565 (-) 435 WP_057789897.1 Holliday junction resolvase RuvX -
  R1T43_RS05320 (R1T43_05320) - 1251558..1252124 (-) 567 WP_317353633.1 YqgE/AlgH family protein -
  R1T43_RS05325 (R1T43_05325) gshB 1252175..1253128 (-) 954 WP_317353636.1 glutathione synthase -
  R1T43_RS05330 (R1T43_05330) - 1253341..1254069 (-) 729 WP_211071661.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38376.13 Da        Isoelectric Point: 6.5064

>NTDB_id=892549 R1T43_RS05300 WP_317353626.1 1248291..1249334(+) (pilT) [Alteromonas sp. CI.11.F.A3]
MDITELLAFSVKNKASDLHLSAGLPPLIRVDGEMRKLNVPALDHKQVHALIYEIMNDMQRKEYEENLETDFSFEISGLSR
FRVNAFVQNRGAAAVLRTIPNTVLTLDDLGAPEIFKEIINQPTGIVLVTGATGSGKSTTLAAMVDHINSHKREHILTIED
PIEFVHENKMSLINQREVHRDTHSFSNALRSALREDPDVILVGELRDLETIRLAISAAETGHLVFGTLHTNSAPKTIDRI
IDVFPAEEKAMVRSMLSESLRAVISQTLLKKVGGGRIAAHEIMVGIPAIRNLIREDKVPQMYSVIQTGQATGMQTMDQCL
QRLLALGAISKEDAAAKSKDKQSMSNF

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=892549 R1T43_RS05300 WP_317353626.1 1248291..1249334(+) (pilT) [Alteromonas sp. CI.11.F.A3]
GTGGATATTACCGAACTTTTGGCTTTCAGCGTTAAAAATAAAGCGTCAGATTTGCACTTATCAGCAGGTCTGCCACCTCT
TATCCGCGTAGACGGAGAAATGCGTAAGCTTAATGTACCCGCATTAGATCATAAACAAGTGCATGCACTTATTTATGAAA
TAATGAATGACATGCAACGTAAAGAATACGAAGAGAACTTGGAAACCGATTTCTCATTCGAAATTAGTGGCTTGTCGCGG
TTTCGTGTAAATGCTTTTGTGCAAAATCGCGGTGCAGCAGCTGTGCTCCGTACTATCCCCAACACGGTTCTTACGCTTGA
TGATTTAGGTGCGCCTGAAATATTCAAAGAAATAATCAACCAACCTACCGGAATTGTTCTAGTCACTGGCGCCACGGGGT
CGGGTAAGAGTACAACCCTTGCCGCGATGGTTGATCATATCAATTCGCACAAGCGTGAGCACATTCTCACTATTGAAGAT
CCTATCGAGTTTGTGCACGAAAATAAAATGAGCTTGATAAACCAACGTGAAGTGCATAGAGACACCCACTCTTTTAGTAA
CGCACTTCGCTCAGCATTACGTGAAGATCCGGACGTGATATTAGTGGGTGAATTACGTGATTTAGAAACTATTCGCTTAG
CCATTTCAGCTGCGGAAACGGGGCATCTAGTATTTGGTACCTTGCATACTAATTCAGCACCTAAAACAATAGACCGCATT
ATCGATGTATTCCCCGCCGAAGAAAAAGCGATGGTGCGCTCAATGTTATCTGAATCGCTAAGAGCTGTAATTTCTCAAAC
CCTGTTAAAAAAAGTGGGGGGAGGGCGAATAGCAGCGCATGAAATCATGGTAGGTATTCCAGCAATTCGAAATCTTATCC
GTGAAGATAAAGTGCCTCAAATGTATTCCGTTATTCAAACGGGGCAAGCTACGGGTATGCAAACTATGGATCAGTGTTTA
CAACGCTTGTTGGCATTAGGTGCAATTTCTAAAGAAGACGCCGCTGCTAAATCGAAAGACAAGCAATCGATGAGTAATTT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

74.709

99.135

0.741
  pilT Acinetobacter baylyi ADP1

73.837

99.135

0.732
  pilT Acinetobacter baumannii D1279779

73.837

99.135

0.732
  pilT Acinetobacter baumannii strain A118

73.837

99.135

0.732
  pilT Pseudomonas aeruginosa PAK

74.926

97.695

0.732
  pilT Acinetobacter nosocomialis M2

73.547

99.135

0.729
  pilT Legionella pneumophila strain Lp02

73.054

96.254

0.703
  pilT Legionella pneumophila strain ERS1305867

73.054

96.254

0.703
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.81

97.118

0.697
  pilT Vibrio cholerae strain A1552

71.81

97.118

0.697
  pilT Neisseria meningitidis 8013

65.318

99.712

0.651
  pilT Neisseria gonorrhoeae MS11

65.029

99.712

0.648
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.846

93.66

0.504
  pilU Acinetobacter baylyi ADP1

42.262

96.83

0.409
  pilU Vibrio cholerae strain A1552

42.09

96.542

0.406
  pilU Pseudomonas stutzeri DSM 10701

41.194

96.542

0.398