Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   R1T43_RS05305 Genome accession   NZ_CP136708
Coordinates   1249344..1250456 (+) Length   370 a.a.
NCBI ID   WP_317353627.1    Uniprot ID   A0AAU0H4R1
Organism   Alteromonas sp. CI.11.F.A3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1244344..1255456
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R1T43_RS05275 (R1T43_05275) - 1244548..1245402 (-) 855 WP_410549000.1 transposase -
  R1T43_RS05280 (R1T43_05280) - 1245679..1246119 (-) 441 WP_317353617.1 DUF4426 domain-containing protein -
  R1T43_RS05285 (R1T43_05285) - 1246142..1246681 (-) 540 WP_013782901.1 YggT family protein -
  R1T43_RS05290 (R1T43_05290) proC 1246703..1247524 (-) 822 WP_317353622.1 pyrroline-5-carboxylate reductase -
  R1T43_RS05295 (R1T43_05295) - 1247580..1248266 (-) 687 WP_317353623.1 YggS family pyridoxal phosphate-dependent enzyme -
  R1T43_RS05300 (R1T43_05300) pilT 1248291..1249334 (+) 1044 WP_317353626.1 type IV pilus twitching motility protein PilT Machinery gene
  R1T43_RS05305 (R1T43_05305) pilU 1249344..1250456 (+) 1113 WP_317353627.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  R1T43_RS05310 (R1T43_05310) - 1250462..1250776 (+) 315 WP_317353629.1 DUF2007 domain-containing protein -
  R1T43_RS05315 (R1T43_05315) ruvX 1251131..1251565 (-) 435 WP_057789897.1 Holliday junction resolvase RuvX -
  R1T43_RS05320 (R1T43_05320) - 1251558..1252124 (-) 567 WP_317353633.1 YqgE/AlgH family protein -
  R1T43_RS05325 (R1T43_05325) gshB 1252175..1253128 (-) 954 WP_317353636.1 glutathione synthase -
  R1T43_RS05330 (R1T43_05330) - 1253341..1254069 (-) 729 WP_211071661.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  R1T43_RS05335 (R1T43_05335) - 1254217..1254381 (-) 165 WP_013782911.1 hypothetical protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40815.51 Da        Isoelectric Point: 5.2675

>NTDB_id=892550 R1T43_RS05305 WP_317353627.1 1249344..1250456(+) (pilU) [Alteromonas sp. CI.11.F.A3]
MLDSFLEKMVEKSASDLFVTAGFPVSAKVHGKLTPISENSFSDEDALALVHEAMNEKQKEAFHTTKECNFAIVREGLGRF
RCSAFWQRDQAGMVVRRIVTEIPKVDDLGLPSVLKDIIMSKRGLVLFVGGTGTGKSTSLAALIGHRNSNSFGHILTIEDP
IEFVHEHQSCVITQREVGIDTHSFDDALKSSLRQAPDVILIGEIRSMETMEYAMSFADTGHLCVATLHANNANQAIERIM
HLAPKDQHDKLRFDLSQNIKAIVAQQLLPTKDGEGRVAAIEILLNSPLVSDLIQRNEIGSLKEAMKKGKELGMQSFDMAL
YDLYREGSIDLDQALHHADSPNDLRLMIKLDSNDGSSLGTLSNVSIDMDD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=892550 R1T43_RS05305 WP_317353627.1 1249344..1250456(+) (pilU) [Alteromonas sp. CI.11.F.A3]
ATGTTAGATAGCTTTTTAGAAAAAATGGTAGAGAAATCTGCTTCTGACTTATTCGTTACCGCGGGCTTTCCCGTCAGCGC
GAAGGTGCACGGTAAGCTTACACCCATTTCGGAAAATTCATTTAGTGATGAAGACGCATTAGCACTTGTACATGAGGCCA
TGAATGAAAAGCAAAAAGAGGCTTTTCATACTACCAAGGAATGTAACTTTGCCATTGTACGAGAAGGCCTAGGGCGTTTT
CGTTGTTCAGCATTTTGGCAAAGAGATCAAGCCGGAATGGTGGTACGTCGTATTGTGACCGAAATTCCTAAGGTCGATGA
CCTGGGGTTACCTTCGGTGCTAAAAGATATCATTATGTCAAAACGCGGTTTGGTGCTTTTTGTAGGTGGTACTGGTACCG
GTAAATCAACCTCTTTGGCAGCACTTATTGGCCATCGAAATAGTAATTCTTTTGGGCATATTTTAACCATTGAAGATCCA
ATCGAATTTGTGCACGAGCATCAATCTTGCGTGATAACTCAGCGTGAAGTAGGCATAGATACTCATTCATTTGACGATGC
GTTAAAAAGCTCATTGCGACAAGCGCCGGATGTCATCCTTATTGGTGAAATTCGTTCAATGGAGACAATGGAATACGCCA
TGTCGTTTGCTGATACGGGGCATTTGTGTGTGGCGACACTTCATGCCAACAATGCTAACCAAGCCATTGAGCGTATTATG
CACTTAGCGCCAAAAGATCAGCACGACAAGTTACGCTTCGATTTAAGCCAAAATATAAAAGCCATTGTGGCGCAGCAACT
CCTGCCAACCAAAGATGGAGAGGGTAGGGTTGCGGCCATTGAAATCTTATTGAACTCTCCGTTGGTGAGCGACCTTATTC
AACGTAATGAAATAGGTAGCTTGAAAGAAGCCATGAAAAAGGGCAAAGAGTTAGGCATGCAAAGCTTTGACATGGCGCTG
TACGATTTGTATCGTGAAGGCAGTATTGATTTAGACCAAGCTTTACATCATGCGGATTCTCCCAATGATTTACGCCTAAT
GATAAAACTAGACTCTAATGATGGGTCGAGCCTAGGCACATTGTCGAATGTCTCGATAGATATGGATGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(104-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.838

100

0.6
  pilU Vibrio cholerae strain A1552

55.707

99.459

0.554
  pilU Acinetobacter baylyi ADP1

56.164

98.649

0.554
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.21

95.405

0.403
  pilT Acinetobacter nosocomialis M2

40.964

89.73

0.368
  pilT Acinetobacter baumannii D1279779

40.964

89.73

0.368
  pilT Acinetobacter baumannii strain A118

40.964

89.73

0.368