Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OCV04_RS02035 Genome accession   NZ_AP024893
Coordinates   421820..422857 (+) Length   345 a.a.
NCBI ID   WP_171137260.1    Uniprot ID   -
Organism   Vibrio plantisponsor strain CECT 7581     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 416820..427857
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV04_RS02000 hemW 416987..418159 (-) 1173 WP_171137257.1 radical SAM family heme chaperone HemW -
  OCV04_RS02005 - 418159..418758 (-) 600 WP_171137258.1 XTP/dITP diphosphatase -
  OCV04_RS02010 - 418783..419214 (-) 432 WP_171137259.1 DUF4426 domain-containing protein -
  OCV04_RS02015 yggU 419281..419571 (-) 291 WP_102955634.1 DUF167 family protein YggU -
  OCV04_RS02020 - 419571..420128 (-) 558 WP_167413112.1 YggT family protein -
  OCV04_RS02025 proC 420157..420975 (-) 819 WP_102942717.1 pyrroline-5-carboxylate reductase -
  OCV04_RS02030 - 421071..421775 (-) 705 WP_102942716.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCV04_RS02035 pilT 421820..422857 (+) 1038 WP_171137260.1 type IV pilus twitching motility protein PilT Machinery gene
  OCV04_RS02040 pilU 422869..423975 (+) 1107 WP_171137261.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCV04_RS02045 ruvX 424029..424454 (-) 426 WP_042489189.1 Holliday junction resolvase RuvX -
  OCV04_RS02050 - 424527..425090 (-) 564 WP_042489186.1 YqgE/AlgH family protein -
  OCV04_RS02055 gshB 425117..426073 (-) 957 WP_171137262.1 glutathione synthase -
  OCV04_RS02060 rsmE 426086..426817 (-) 732 WP_102942712.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OCV04_RS02065 - 427104..427805 (-) 702 WP_171137263.1 endonuclease -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38266.78 Da        Isoelectric Point: 6.5031

>NTDB_id=89201 OCV04_RS02035 WP_171137260.1 421820..422857(+) (pilT) [Vibrio plantisponsor strain CECT 7581]
MNIAELLDFSVKHNASDLHLSAGVPPMVRIDGEVRKLGVPAFTHADVHRLVFEVMNDAQRSEFEEKLEVDFSFELPDVGR
FRVNAFHQARGCSAVFRTIPTVIPTLDQLDAPEIFRKIANYEKGLVLVTGPTGSGKSTTLAAMVDYINKNHNKHILTIED
PIEFVHSNDKCLINQREVHRDTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGNDKDMVRSMLSESLRAVIAQKLLKRTGGGRVACHEIMLATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMDQNS
KQLIAQGLVDPAEVQKKIESEISTF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=89201 OCV04_RS02035 WP_171137260.1 421820..422857(+) (pilT) [Vibrio plantisponsor strain CECT 7581]
ATGAATATCGCTGAGTTACTGGATTTTAGTGTAAAGCATAATGCGTCAGATCTACATCTTTCTGCAGGTGTTCCGCCTAT
GGTACGTATAGATGGCGAAGTAAGAAAGCTTGGCGTGCCTGCTTTTACCCATGCAGATGTTCATCGTCTAGTGTTTGAAG
TGATGAATGATGCTCAGCGCAGTGAGTTTGAAGAAAAACTCGAAGTGGATTTTTCTTTTGAACTGCCGGATGTAGGGCGC
TTTCGTGTGAATGCATTCCACCAAGCCCGCGGCTGTTCTGCTGTGTTCCGTACTATCCCGACAGTCATTCCAACTCTTGA
CCAGTTAGATGCTCCCGAGATTTTCAGAAAAATTGCCAACTATGAGAAAGGTCTGGTTCTGGTGACAGGCCCAACAGGTT
CTGGTAAATCCACCACTTTGGCTGCGATGGTCGATTACATCAACAAGAATCACAACAAGCATATTTTGACCATTGAAGAT
CCAATCGAGTTCGTTCATAGCAACGATAAGTGTTTGATCAACCAACGCGAAGTTCATCGCGATACGCACAGTTTCAAGAA
TGCACTGCGTTCAGCACTGCGTGAAGATCCAGATGTCATTCTGGTCGGTGAGTTGCGTGACCAAGAAACCATTAGCTTAG
CTCTGACAGCTGCGGAAACAGGACACCTTGTCTTTGGTACGCTGCACACTAGTTCGGCAGCAAAAACCATTGACCGTATT
ATTGATGTGTTTCCGGGCAACGATAAAGATATGGTGCGTTCTATGTTGTCTGAATCCTTACGCGCGGTGATTGCACAGAA
ACTGCTAAAACGTACTGGCGGAGGTCGAGTTGCTTGCCATGAGATCATGCTGGCGACACCTGCAATTCGTAACTTGATCC
GCGAAGACAAAGTGGCTCAGATGTATTCGATCATCCAAACCGGTGCTGCTCACGGAATGCAAACCATGGATCAAAACTCC
AAACAGCTGATTGCTCAAGGTTTAGTGGATCCCGCTGAAGTACAGAAGAAGATTGAGTCTGAAATCTCTACCTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

91.884

100

0.919
  pilT Vibrio cholerae strain A1552

91.884

100

0.919
  pilT Acinetobacter baumannii D1279779

72.205

95.942

0.693
  pilT Acinetobacter baumannii strain A118

72.205

95.942

0.693
  pilT Acinetobacter nosocomialis M2

71.903

95.942

0.69
  pilT Acinetobacter baylyi ADP1

71.212

95.652

0.681
  pilT Pseudomonas stutzeri DSM 10701

68.843

97.681

0.672
  pilT Pseudomonas aeruginosa PAK

67.359

97.681

0.658
  pilT Legionella pneumophila strain Lp02

66.565

95.362

0.635
  pilT Legionella pneumophila strain ERS1305867

66.565

95.362

0.635
  pilT Neisseria meningitidis 8013

63.112

100

0.635
  pilT Neisseria gonorrhoeae MS11

62.824

100

0.632
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.774

92.174

0.496
  pilU Vibrio cholerae strain A1552

44

94.203

0.414
  pilU Pseudomonas stutzeri DSM 10701

42.683

95.072

0.406
  pilU Acinetobacter baylyi ADP1

40.312

92.754

0.374

Multiple sequence alignment