Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OCV04_RS02040 Genome accession   NZ_AP024893
Coordinates   422869..423975 (+) Length   368 a.a.
NCBI ID   WP_171137261.1    Uniprot ID   -
Organism   Vibrio plantisponsor strain CECT 7581     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 417869..428975
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV04_RS02005 - 418159..418758 (-) 600 WP_171137258.1 XTP/dITP diphosphatase -
  OCV04_RS02010 - 418783..419214 (-) 432 WP_171137259.1 DUF4426 domain-containing protein -
  OCV04_RS02015 yggU 419281..419571 (-) 291 WP_102955634.1 DUF167 family protein YggU -
  OCV04_RS02020 - 419571..420128 (-) 558 WP_167413112.1 YggT family protein -
  OCV04_RS02025 proC 420157..420975 (-) 819 WP_102942717.1 pyrroline-5-carboxylate reductase -
  OCV04_RS02030 - 421071..421775 (-) 705 WP_102942716.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCV04_RS02035 pilT 421820..422857 (+) 1038 WP_171137260.1 type IV pilus twitching motility protein PilT Machinery gene
  OCV04_RS02040 pilU 422869..423975 (+) 1107 WP_171137261.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCV04_RS02045 ruvX 424029..424454 (-) 426 WP_042489189.1 Holliday junction resolvase RuvX -
  OCV04_RS02050 - 424527..425090 (-) 564 WP_042489186.1 YqgE/AlgH family protein -
  OCV04_RS02055 gshB 425117..426073 (-) 957 WP_171137262.1 glutathione synthase -
  OCV04_RS02060 rsmE 426086..426817 (-) 732 WP_102942712.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OCV04_RS02065 - 427104..427805 (-) 702 WP_171137263.1 endonuclease -
  OCV04_RS02070 - 427942..428445 (-) 504 WP_171137264.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41378.41 Da        Isoelectric Point: 6.5070

>NTDB_id=89202 OCV04_RS02040 WP_171137261.1 422869..423975(+) (pilU) [Vibrio plantisponsor strain CECT 7581]
MELNHYLEAMTSLKASDLYITVGAPVLLRVDGEMRPQGEKLSQQEVLSLLHEMMDEERKAEFNASHEANFAIVRGNGRFR
VSAFFQRELPGAVIRRIETQIPTFEELHLPAVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNRHRSGHILTVEDPI
EFVHEHQKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLRGVVGQQLIRDKNGKGRHGVFEILLNSPRVSDLIRKGELHELKSTMAKSKEIGMQTFDQALY
QLLVEDKITEQDAMHSADSANDLRMMLKTQRGDTPSSSSLANVKIDMN

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=89202 OCV04_RS02040 WP_171137261.1 422869..423975(+) (pilU) [Vibrio plantisponsor strain CECT 7581]
ATGGAATTGAATCACTACCTTGAAGCGATGACCTCGCTCAAAGCGTCGGACTTATATATTACTGTCGGTGCACCGGTATT
GTTGCGTGTCGATGGCGAGATGCGTCCGCAAGGGGAGAAGCTTTCACAGCAAGAGGTACTTTCTTTATTACATGAAATGA
TGGATGAAGAGCGTAAGGCTGAGTTTAACGCATCGCACGAAGCGAACTTTGCCATCGTAAGAGGAAATGGACGTTTCAGG
GTGAGTGCTTTTTTCCAACGTGAGCTGCCGGGTGCAGTTATTCGACGTATCGAAACGCAAATTCCGACATTTGAAGAATT
GCACTTGCCTGCTGTTTTGCAGGATCTTGCGATTGCAAAGCGAGGCTTAGTGCTGGTCGTGGGGGCTACGGGTTCGGGTA
AATCCACCACCATGGCAGCTATGACGGGGTATCGTAATCGTCATCGCAGTGGACATATCTTAACGGTTGAAGATCCGATT
GAGTTCGTACACGAACATCAAAAATGCATCGTCACTCAGCGAGAAGTGGGGTTAGATACCGAAAGCTACGAAGTGGCACT
GAAGAACTCTCTTCGCCAAGCGCCAGACATGATTTTGATTGGTGAAATCCGCAGTCGTGAAACCATGGAATACGCGATGA
CCTTTGCTGAAACAGGGCACTTGTGTATGGCAACCCTGCATGCGAACAATGCTAACCAAGCGTTAGAGCGTATTCTTCAC
CTAGTACCTAAAGAACAAAAAGAGCAGTTCTTGTTTGATTTGTCGATGAACTTACGTGGCGTGGTAGGGCAGCAACTGAT
TCGTGATAAAAATGGTAAAGGTCGCCACGGTGTGTTTGAGATCTTACTCAATTCACCTCGCGTTTCAGATTTGATTCGCA
AAGGAGAGTTACACGAGTTGAAATCGACGATGGCTAAATCGAAAGAGATTGGTATGCAGACGTTTGATCAAGCGTTGTAT
CAGTTACTAGTGGAAGACAAGATCACTGAACAAGATGCAATGCACAGCGCCGATTCTGCCAATGATTTGCGCATGATGTT
AAAAACTCAGCGTGGAGACACTCCGAGTTCAAGTTCACTGGCTAATGTGAAAATCGATATGAACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

83.379

99.728

0.832
  pilU Pseudomonas stutzeri DSM 10701

59.143

95.109

0.563
  pilU Acinetobacter baylyi ADP1

53.846

95.38

0.514
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.308

88.315

0.391
  pilT Acinetobacter baylyi ADP1

42.229

92.663

0.391
  pilT Legionella pneumophila strain ERS1305867

41.593

92.12

0.383
  pilT Legionella pneumophila strain Lp02

41.593

92.12

0.383
  pilT Vibrio cholerae strain A1552

41.617

90.761

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.617

90.761

0.378
  pilT Pseudomonas aeruginosa PAK

40.708

92.12

0.375
  pilT Acinetobacter baumannii D1279779

40.413

92.12

0.372
  pilT Acinetobacter baumannii strain A118

40.413

92.12

0.372
  pilT Acinetobacter nosocomialis M2

40.413

92.12

0.372
  pilT Pseudomonas stutzeri DSM 10701

40.118

92.12

0.37

Multiple sequence alignment