Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RZV17_RS14010 Genome accession   NZ_CP136580
Coordinates   3289764..3290801 (-) Length   345 a.a.
NCBI ID   WP_003489919.1    Uniprot ID   -
Organism   Xanthomonas cannabis strain DAR41331     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3284764..3295801
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RZV17_RS13985 (RZV17_13945) - 3285146..3285712 (-) 567 WP_039404799.1 YqgE/AlgH family protein -
  RZV17_RS13990 (RZV17_13950) - 3286021..3286587 (+) 567 WP_179454303.1 DNA-3-methyladenine glycosylase I -
  RZV17_RS13995 (RZV17_13955) - 3286624..3287523 (+) 900 WP_355604317.1 DUF72 domain-containing protein -
  RZV17_RS14000 (RZV17_13960) - 3287536..3288210 (-) 675 WP_039566430.1 YitT family protein -
  RZV17_RS14005 (RZV17_13965) pilU 3288520..3289650 (-) 1131 WP_039404792.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RZV17_RS14010 (RZV17_13970) pilT 3289764..3290801 (-) 1038 WP_003489919.1 type IV pilus twitching motility protein PilT Machinery gene
  RZV17_RS14015 (RZV17_13975) - 3291129..3291821 (+) 693 WP_166745300.1 YggS family pyridoxal phosphate-dependent enzyme -
  RZV17_RS14020 (RZV17_13980) proC 3291865..3292719 (+) 855 WP_104579947.1 pyrroline-5-carboxylate reductase -
  RZV17_RS14025 (RZV17_13985) - 3293276..3293695 (+) 420 WP_039404742.1 HU family DNA-binding protein -
  RZV17_RS14030 (RZV17_13990) - 3293845..3294429 (+) 585 WP_355604323.1 M23 family metallopeptidase -
  RZV17_RS14035 (RZV17_13995) - 3294521..3294823 (+) 303 WP_047693789.1 DUF3861 family protein -
  RZV17_RS14040 (RZV17_14000) - 3294995..3295423 (+) 429 WP_355609392.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38643.30 Da        Isoelectric Point: 6.6652

>NTDB_id=891698 RZV17_RS14010 WP_003489919.1 3289764..3290801(-) (pilT) [Xanthomonas cannabis strain DAR41331]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLIDQPQGLILVTGPTGSGKSTTLAGMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSSIQTGQQYGMQTLDQHL
QDLVKRSLITRNQAREYAKDKRIFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=891698 RZV17_RS14010 WP_003489919.1 3289764..3290801(-) (pilT) [Xanthomonas cannabis strain DAR41331]
ATGGATATCGCTGAACTATTGGCGTTTTCTGTCAAGAACAAGGCATCGGACCTGCACCTGTCTGCAGGGCTGCCGCCGAT
GATCCGTGTCGATGGCGATGTCCGCCGCATCAATATTCCGGCGCTGGACCACAAGCAGGTGCATGCGCTGGTCTACGACA
TCATGTCGGACAAGCAGCGCCGCGATTACGAAGAATTCCTCGAGGTCGACTTCTCGTTCGAGATCCCGTCGCTGGCGCGC
TTCCGCGTCAATGCGTTCAATCAGAACCGCGGCGCCGGTGCGGTGTTCCGTACCATTCCCTCCGAAGTGCTGACGCTGGA
AGACCTGGGCTGCCCGCCGATCTTCCGCCAGCTGATCGACCAGCCGCAGGGCCTGATCCTGGTCACGGGCCCCACCGGGT
CGGGCAAGTCGACCACGCTGGCCGGCATGATCGACTACATCAACAAGAACGAATACGGCCACATCCTCACTGTCGAGGAC
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCACCGCGACACGCACGGCTTCAACGA
GGCGCTGCGCTCGGCACTGCGCGAAGACCCGGACATCATCCTTGTGGGCGAATTGCGCGACCTGGAGACCATCCGCCTGG
CGCTCACCGCCGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAGTCGCTGCGCGCGGTCATTTCGCAGGC
GCTGCTGAAGAAGGTGGGCGGCGGACGCACCGCCGCCTGGGAAATCATGGTCGGCACCCCGGCCATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCCTCGATCCAGACCGGCCAGCAATACGGCATGCAGACGCTGGACCAGCATCTG
CAGGACCTGGTCAAGCGCAGCCTGATCACGCGCAACCAGGCGCGTGAGTACGCGAAGGACAAGCGGATATTCGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.942

99.71

0.797
  pilT Acinetobacter baumannii D1279779

79.942

99.71

0.797
  pilT Acinetobacter baumannii strain A118

79.942

99.71

0.797
  pilT Pseudomonas stutzeri DSM 10701

79.36

99.71

0.791
  pilT Acinetobacter baylyi ADP1

78.779

99.71

0.786
  pilT Pseudomonas aeruginosa PAK

79.056

98.261

0.777
  pilT Legionella pneumophila strain Lp02

75.291

99.71

0.751
  pilT Legionella pneumophila strain ERS1305867

75.291

99.71

0.751
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.125

95.362

0.678
  pilT Vibrio cholerae strain A1552

71.125

95.362

0.678
  pilT Neisseria meningitidis 8013

65.982

98.841

0.652
  pilT Neisseria gonorrhoeae MS11

65.689

98.841

0.649
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

100

0.499
  pilU Pseudomonas stutzeri DSM 10701

40.708

98.261

0.4
  pilU Acinetobacter baylyi ADP1

40.294

98.551

0.397
  pilU Vibrio cholerae strain A1552

40.597

97.101

0.394