Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OCV27_RS02070 Genome accession   NZ_AP024889
Coordinates   427180..428217 (+) Length   345 a.a.
NCBI ID   WP_125322945.1    Uniprot ID   A0A3R9F640
Organism   Vibrio pectenicida strain LMG 19642     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 422180..433217
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV27_RS02040 hemW 422555..423733 (-) 1179 WP_125322951.1 radical SAM family heme chaperone HemW -
  OCV27_RS02045 - 423746..424351 (-) 606 WP_125322950.1 XTP/dITP diphosphatase -
  OCV27_RS02050 - 424380..424811 (-) 432 WP_125322949.1 DUF4426 domain-containing protein -
  OCV27_RS02055 - 424996..425553 (-) 558 WP_125322948.1 YggT family protein -
  OCV27_RS02060 proC 425593..426411 (-) 819 WP_125322947.1 pyrroline-5-carboxylate reductase -
  OCV27_RS02065 - 426436..427155 (-) 720 WP_125322946.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCV27_RS02070 pilT 427180..428217 (+) 1038 WP_125322945.1 type IV pilus twitching motility protein PilT Machinery gene
  OCV27_RS02075 pilU 428229..429335 (+) 1107 WP_125322944.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCV27_RS02080 ruvX 429355..429777 (-) 423 WP_125322943.1 Holliday junction resolvase RuvX -
  OCV27_RS02085 - 429795..430358 (-) 564 WP_125322942.1 YqgE/AlgH family protein -
  OCV27_RS02090 gshB 430415..431365 (-) 951 WP_125322941.1 glutathione synthase -
  OCV27_RS02095 rsmE 431378..432109 (-) 732 WP_125322940.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OCV27_RS02100 - 432275..432763 (-) 489 WP_125322939.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 37972.39 Da        Isoelectric Point: 6.1084

>NTDB_id=89167 OCV27_RS02070 WP_125322945.1 427180..428217(+) (pilT) [Vibrio pectenicida strain LMG 19642]
MDIAELLDFSVKHNASDLHLSAGVSPMIRIDGDVRKLGVPAFNHSEVHRLVTEIMNDSQRSEFEEHLEIDFSFELPDVGR
FRVNAFNQSRGCGAVFRTIPTVIPTLEQLEAPATFENIANMEKGLILVTGPTGSGKSTTLAAMVDYINRNHNKHILTIED
PIEFVHTNNKCLLNQREVHKDTHSFNNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKGMVRSMLSESLRAVIAQKLLKKIGGGRVACHEIMMATPAIRNLIREDKVAQMQSVIQTGAAHGMQTMEQHS
KQLIAQGLVDAEEVAKKIEAESGMI

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=89167 OCV27_RS02070 WP_125322945.1 427180..428217(+) (pilT) [Vibrio pectenicida strain LMG 19642]
ATGGATATCGCTGAATTACTGGACTTTAGTGTAAAGCATAATGCGTCAGATCTACATCTTTCTGCTGGAGTATCTCCGAT
GATACGTATTGATGGTGATGTAAGAAAGTTAGGTGTGCCCGCTTTTAATCACTCTGAGGTCCATCGCTTAGTGACTGAAA
TCATGAACGACTCTCAACGTAGTGAATTTGAGGAACATTTAGAAATTGATTTTTCTTTTGAGCTGCCAGATGTTGGACGT
TTTCGGGTCAATGCATTTAATCAATCGAGAGGATGTGGTGCGGTATTTCGTACGATCCCGACCGTCATTCCGACCTTGGA
GCAGCTAGAGGCTCCGGCAACTTTTGAAAATATCGCCAATATGGAGAAAGGTTTAATCCTTGTTACTGGGCCTACTGGTT
CGGGTAAATCCACAACACTGGCCGCTATGGTGGATTATATTAATCGTAACCATAACAAGCATATTCTGACGATTGAGGAT
CCGATCGAATTTGTGCATACCAACAATAAATGCTTGCTAAATCAGCGTGAAGTCCACAAAGACACGCATAGTTTTAACAA
TGCTTTGCGCAGTGCTTTGCGAGAAGATCCTGACGTAATTTTGGTTGGTGAGTTGCGAGATCAAGAAACTATAAGCCTTG
CCCTTACCGCAGCAGAAACTGGGCATTTGGTGTTTGGTACTTTACATACCAGTTCTGCAGCTAAAACTATCGATCGTATC
ATTGATGTATTCCCCGGCAGTGATAAAGGTATGGTTCGCTCCATGCTTTCGGAGTCACTTAGAGCTGTCATTGCGCAGAA
ACTACTGAAAAAAATTGGTGGGGGTCGAGTGGCTTGTCATGAAATCATGATGGCGACACCTGCTATCAGAAACTTGATTC
GTGAAGATAAAGTCGCCCAGATGCAATCTGTTATTCAGACGGGTGCAGCTCACGGCATGCAGACTATGGAGCAACACTCG
AAGCAATTGATTGCTCAGGGACTGGTTGATGCAGAAGAGGTGGCGAAAAAAATTGAAGCTGAGTCTGGGATGATATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3R9F640

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

87.021

98.261

0.855
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

87.021

98.261

0.855
  pilT Acinetobacter baylyi ADP1

69.388

99.42

0.69
  pilT Acinetobacter baumannii D1279779

69.388

99.42

0.69
  pilT Acinetobacter baumannii strain A118

69.388

99.42

0.69
  pilT Acinetobacter nosocomialis M2

69.096

99.42

0.687
  pilT Pseudomonas stutzeri DSM 10701

69.006

99.13

0.684
  pilT Pseudomonas aeruginosa PAK

68.546

97.681

0.67
  pilT Legionella pneumophila strain Lp02

68.389

95.362

0.652
  pilT Legionella pneumophila strain ERS1305867

68.389

95.362

0.652
  pilT Neisseria meningitidis 8013

65.089

97.971

0.638
  pilT Neisseria gonorrhoeae MS11

62.899

100

0.629
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.818

95.652

0.496
  pilU Vibrio cholerae strain A1552

42.462

94.203

0.4
  pilU Pseudomonas stutzeri DSM 10701

41.018

96.812

0.397
  pilU Acinetobacter baylyi ADP1

40

92.754

0.371

Multiple sequence alignment