Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OCV27_RS02075 Genome accession   NZ_AP024889
Coordinates   428229..429335 (+) Length   368 a.a.
NCBI ID   WP_125322944.1    Uniprot ID   A0A3R9FK21
Organism   Vibrio pectenicida strain LMG 19642     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 423229..434335
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV27_RS02045 - 423746..424351 (-) 606 WP_125322950.1 XTP/dITP diphosphatase -
  OCV27_RS02050 - 424380..424811 (-) 432 WP_125322949.1 DUF4426 domain-containing protein -
  OCV27_RS02055 - 424996..425553 (-) 558 WP_125322948.1 YggT family protein -
  OCV27_RS02060 proC 425593..426411 (-) 819 WP_125322947.1 pyrroline-5-carboxylate reductase -
  OCV27_RS02065 - 426436..427155 (-) 720 WP_125322946.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCV27_RS02070 pilT 427180..428217 (+) 1038 WP_125322945.1 type IV pilus twitching motility protein PilT Machinery gene
  OCV27_RS02075 pilU 428229..429335 (+) 1107 WP_125322944.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCV27_RS02080 ruvX 429355..429777 (-) 423 WP_125322943.1 Holliday junction resolvase RuvX -
  OCV27_RS02085 - 429795..430358 (-) 564 WP_125322942.1 YqgE/AlgH family protein -
  OCV27_RS02090 gshB 430415..431365 (-) 951 WP_125322941.1 glutathione synthase -
  OCV27_RS02095 rsmE 431378..432109 (-) 732 WP_125322940.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OCV27_RS02100 - 432275..432763 (-) 489 WP_125322939.1 SprT family zinc-dependent metalloprotease -
  OCV27_RS02105 - 432828..433625 (-) 798 WP_125322938.1 DUF2189 domain-containing protein -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 40866.65 Da        Isoelectric Point: 6.3180

>NTDB_id=89168 OCV27_RS02075 WP_125322944.1 428229..429335(+) (pilU) [Vibrio pectenicida strain LMG 19642]
MDINACLQGMLSEKASDLYITVGAPLLYRVNGDLKPLGDSLSQQAVSALLHNVMDESRQSEFAQSREANFAIVKDFGRFR
ISAFYQRELPGAVIRRIETTIPTFDELKLPEILKDLSIAKRGLVLVVGATGSGKSTTMAAMTGHRNNHKSGHILTVEDPI
EFVHEHGKCIVTQREVGLDTNSYEVALKNSLRQAPDMILIGEIRSRETMEFAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLRGVVAQQLLRDKHGNGRHGVFEILLNSPRVSDLIRRGDLHELKNTMGKSQQSGMQTFDQALY
QLLIDDKIAEDDALHSADSANDLRIMLKTQRGESFGGGALADVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=89168 OCV27_RS02075 WP_125322944.1 428229..429335(+) (pilU) [Vibrio pectenicida strain LMG 19642]
ATGGATATCAATGCTTGCCTGCAAGGTATGTTGAGTGAGAAGGCATCGGACTTATATATTACTGTCGGCGCTCCTCTTCT
ATATCGAGTTAATGGTGATTTAAAACCGCTTGGTGATAGTTTGTCACAGCAAGCAGTATCAGCATTATTACATAATGTAA
TGGATGAGAGTCGCCAGAGTGAATTTGCTCAGAGCAGAGAAGCTAATTTTGCGATTGTAAAAGACTTTGGTCGTTTTCGA
ATTTCAGCTTTTTACCAGCGTGAGTTACCGGGAGCAGTAATTCGTCGTATTGAAACAACTATTCCCACCTTTGATGAGCT
TAAATTACCTGAAATATTAAAAGATTTATCTATAGCTAAGCGTGGTTTAGTCTTGGTTGTTGGTGCGACTGGTTCGGGTA
AATCAACCACTATGGCTGCAATGACTGGGCACCGAAATAATCATAAAAGTGGGCATATTTTAACGGTTGAAGATCCAATA
GAATTTGTCCATGAGCATGGTAAATGTATCGTTACTCAGAGGGAAGTTGGTCTTGATACAAACAGCTATGAAGTCGCTTT
GAAAAACTCCTTGCGACAGGCTCCTGATATGATCTTGATTGGTGAAATTCGGTCTCGGGAGACGATGGAGTTTGCGATGA
CGTTTGCAGAAACAGGCCATCTTTGCATGGCAACCTTACACGCGAATAATGCTAATCAAGCACTTGAGCGTATTCTTCAT
CTTGTCCCTAAAGAGCAAAAAGAGCAGTTTTTATTTGATCTTTCTATGAACTTAAGAGGCGTGGTTGCTCAGCAACTACT
GCGCGATAAACATGGTAATGGTCGCCACGGCGTCTTTGAGATTTTGCTAAACAGCCCTAGGGTTTCGGATCTAATACGTC
GTGGCGATCTGCATGAACTCAAAAACACCATGGGAAAATCGCAGCAATCGGGTATGCAGACTTTCGATCAAGCTCTATAT
CAATTACTTATAGACGATAAGATCGCAGAAGATGATGCGCTTCACAGTGCCGACTCAGCGAATGACTTGCGCATCATGCT
CAAAACTCAGCGTGGCGAGAGTTTTGGGGGTGGGGCTTTAGCTGACGTCAAAATTGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3R9FK21

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

79.62

100

0.796
  pilU Pseudomonas stutzeri DSM 10701

56.857

95.109

0.541
  pilU Acinetobacter baylyi ADP1

52.137

95.38

0.497
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.953

92.12

0.405
  pilT Legionella pneumophila strain Lp02

41.298

92.12

0.38
  pilT Legionella pneumophila strain ERS1305867

41.298

92.12

0.38
  pilT Pseudomonas aeruginosa PAK

40.708

92.12

0.375
  pilT Pseudomonas stutzeri DSM 10701

40.413

92.12

0.372
  pilT Acinetobacter baylyi ADP1

41.538

88.315

0.367
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.538

88.315

0.367
  pilT Neisseria meningitidis 8013

39.13

93.75

0.367
  pilT Neisseria gonorrhoeae MS11

39.13

93.75

0.367
  pilT Vibrio cholerae strain A1552

41.538

88.315

0.367

Multiple sequence alignment