Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OCU30_RS01880 Genome accession   NZ_AP024887
Coordinates   387079..388119 (+) Length   346 a.a.
NCBI ID   WP_077315337.1    Uniprot ID   A0A1R4B7Q4
Organism   Vibrio palustris strain CECT 9027     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 382079..393119
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCU30_RS01855 hemW 382891..384066 (-) 1176 WP_077315332.1 radical SAM family heme chaperone HemW -
  OCU30_RS01860 - 384066..384668 (-) 603 WP_077315333.1 XTP/dITP diphosphatase -
  OCU30_RS01865 - 384862..385419 (-) 558 WP_077315334.1 YggT family protein -
  OCU30_RS01870 proC 385487..386305 (-) 819 WP_077315335.1 pyrroline-5-carboxylate reductase -
  OCU30_RS01875 - 386342..387052 (-) 711 WP_077315336.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCU30_RS01880 pilT 387079..388119 (+) 1041 WP_077315337.1 type IV pilus twitching motility protein PilT Machinery gene
  OCU30_RS01885 pilU 388131..389237 (+) 1107 WP_077315338.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCU30_RS01890 ruvX 389329..389754 (-) 426 WP_077315339.1 Holliday junction resolvase RuvX -
  OCU30_RS01895 - 389797..390360 (-) 564 WP_077315340.1 YqgE/AlgH family protein -
  OCU30_RS01900 gshB 390411..391358 (-) 948 WP_077315341.1 glutathione synthase -
  OCU30_RS01905 rsmE 391371..392105 (-) 735 WP_077315342.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OCU30_RS01910 - 392252..392752 (-) 501 WP_077315343.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38168.49 Da        Isoelectric Point: 6.5035

>NTDB_id=89132 OCU30_RS01880 WP_077315337.1 387079..388119(+) (pilT) [Vibrio palustris strain CECT 9027]
MDIAELLDFSVKHNASDLHLSAGVPPMVRIDGDVRKLGIPAFSHSEVHRLIFDIMNDAQQREYEEKLEVDFSFELPSVGR
FRVNAFHQARGSSAVFRTIPTEIPTLSQLDAPEIFNRISHYEKGLVLVTGPTGSGKSTTLAAMVNEINQTKNKHILTIED
PIEFVHTNNKCLINQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFSGADKGMVRSMLSESLRAVIAQNLLKKSEGGRVACHEIMLATPAIRNLIREDKVAQMQSVIQTGSAHGMQTLEQNA
RQLLAKGIVDSEAVAHMIPTDTSTHF

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=89132 OCU30_RS01880 WP_077315337.1 387079..388119(+) (pilT) [Vibrio palustris strain CECT 9027]
ATGGATATCGCTGAATTACTCGATTTTAGTGTAAAACATAATGCCTCAGATTTGCACCTTTCAGCAGGCGTTCCTCCGAT
GGTACGTATTGATGGTGATGTGCGAAAGCTAGGTATCCCTGCCTTTTCTCATTCCGAAGTTCATCGTCTGATCTTTGACA
TCATGAATGACGCTCAGCAACGTGAATACGAAGAAAAACTCGAAGTCGATTTCTCTTTTGAGTTGCCCAGTGTTGGGCGC
TTTCGTGTCAATGCGTTCCATCAAGCACGTGGTAGCTCGGCGGTCTTTCGAACTATCCCTACTGAGATTCCTACTCTATC
ACAGTTGGATGCGCCGGAAATCTTTAATCGTATTAGTCACTATGAAAAAGGATTAGTTCTGGTAACCGGGCCTACGGGGT
CTGGTAAGTCCACCACATTAGCCGCGATGGTCAATGAAATTAATCAAACCAAAAATAAACATATTCTTACCATTGAAGAT
CCGATTGAATTTGTCCATACCAATAATAAATGCCTGATTAATCAAAGAGAAGTACACCGTGATACACACAGTTTCAAAGC
GGCATTACGTTCGGCATTGCGTGAAGATCCGGATGTGATTTTAGTCGGTGAACTACGTGACCAAGAAACAATTAGCTTAG
CGTTAACGGCCGCAGAAACCGGGCATTTGGTATTTGGCACATTGCATACTAGCTCTGCAGCGAAAACAATTGACCGGATT
ATTGATGTCTTCTCTGGTGCTGATAAGGGCATGGTACGCTCGATGTTGTCCGAATCGTTACGGGCAGTGATAGCGCAAAA
TTTGCTTAAAAAGAGTGAAGGTGGCCGGGTCGCGTGCCATGAAATTATGTTGGCCACCCCCGCCATTCGTAACTTGATTC
GTGAAGATAAAGTCGCGCAAATGCAATCTGTCATCCAGACAGGTTCAGCACACGGAATGCAAACGTTAGAGCAAAATGCG
CGGCAATTATTAGCCAAAGGGATCGTCGATAGTGAGGCTGTCGCTCACATGATTCCCACTGACACCTCGACTCATTTCTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1R4B7Q4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

86.51

98.555

0.853
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

86.51

98.555

0.853
  pilT Acinetobacter baylyi ADP1

71.515

95.376

0.682
  pilT Acinetobacter baumannii D1279779

69.578

95.954

0.668
  pilT Acinetobacter baumannii strain A118

69.578

95.954

0.668
  pilT Pseudomonas stutzeri DSM 10701

69.069

96.243

0.665
  pilT Acinetobacter nosocomialis M2

69.277

95.954

0.665
  pilT Pseudomonas aeruginosa PAK

68.976

95.954

0.662
  pilT Legionella pneumophila strain Lp02

67.262

97.11

0.653
  pilT Legionella pneumophila strain ERS1305867

67.262

97.11

0.653
  pilT Neisseria meningitidis 8013

63.664

96.243

0.613
  pilT Neisseria gonorrhoeae MS11

63.473

96.532

0.613
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.145

91.908

0.488
  pilU Vibrio cholerae strain A1552

41.246

97.399

0.402
  pilU Pseudomonas stutzeri DSM 10701

39.349

97.688

0.384
  pilU Acinetobacter baylyi ADP1

39.688

92.486

0.367

Multiple sequence alignment