Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OCU30_RS01885 Genome accession   NZ_AP024887
Coordinates   388131..389237 (+) Length   368 a.a.
NCBI ID   WP_077315338.1    Uniprot ID   A0A1R4B7P8
Organism   Vibrio palustris strain CECT 9027     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 383131..394237
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCU30_RS01860 - 384066..384668 (-) 603 WP_077315333.1 XTP/dITP diphosphatase -
  OCU30_RS01865 - 384862..385419 (-) 558 WP_077315334.1 YggT family protein -
  OCU30_RS01870 proC 385487..386305 (-) 819 WP_077315335.1 pyrroline-5-carboxylate reductase -
  OCU30_RS01875 - 386342..387052 (-) 711 WP_077315336.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCU30_RS01880 pilT 387079..388119 (+) 1041 WP_077315337.1 type IV pilus twitching motility protein PilT Machinery gene
  OCU30_RS01885 pilU 388131..389237 (+) 1107 WP_077315338.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCU30_RS01890 ruvX 389329..389754 (-) 426 WP_077315339.1 Holliday junction resolvase RuvX -
  OCU30_RS01895 - 389797..390360 (-) 564 WP_077315340.1 YqgE/AlgH family protein -
  OCU30_RS01900 gshB 390411..391358 (-) 948 WP_077315341.1 glutathione synthase -
  OCU30_RS01905 rsmE 391371..392105 (-) 735 WP_077315342.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OCU30_RS01910 - 392252..392752 (-) 501 WP_077315343.1 SprT family zinc-dependent metalloprotease -
  OCU30_RS01915 metK 392842..393996 (-) 1155 WP_077315344.1 methionine adenosyltransferase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41433.24 Da        Isoelectric Point: 6.0541

>NTDB_id=89133 OCU30_RS01885 WP_077315338.1 388131..389237(+) (pilU) [Vibrio palustris strain CECT 9027]
MLLEQCLSEMTARKSSDLYITVGAPVLYRVDGELQSMGDKLSLADVNNFLTAMMDSDMSKDFVRTHEANFALVREAGRFR
VSAFYQRELPGAVVRHIETRIPTFAELQLPERFKELVMNKRGLVLVVGATGSGKSTTMAAMTGYRNRHSSGHILTVEDPI
EFVHEHDQCIVTQREVGLDTQSYEIALKNSLRQAPDMILIGEIRSQETMEYAMNFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLRGVVGQQLVRDKDGSGRHGVFEILLNTPRISDLIRRGELHELKETMKRSRESGMQTFDQALY
QLVIDGKITEQDAMHHADSANDLRLLLKGQQGSSYSASSLSDIKIDME

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=89133 OCU30_RS01885 WP_077315338.1 388131..389237(+) (pilU) [Vibrio palustris strain CECT 9027]
ATGTTGCTTGAACAATGTTTATCAGAAATGACCGCGCGCAAGTCCTCGGATCTTTACATTACGGTTGGTGCTCCGGTGTT
ATATCGAGTGGATGGCGAGTTACAAAGCATGGGGGATAAGTTGTCATTAGCAGACGTTAATAACTTTTTAACCGCCATGA
TGGATAGCGACATGAGTAAAGACTTTGTTCGCACTCACGAGGCTAATTTTGCGTTAGTTCGTGAGGCTGGACGATTTCGG
GTCAGTGCTTTTTATCAGCGCGAGTTGCCAGGGGCGGTGGTTCGCCATATTGAAACCCGCATCCCGACTTTTGCAGAGTT
ACAACTCCCTGAGCGCTTCAAAGAGTTAGTGATGAATAAGCGTGGCTTGGTATTAGTAGTGGGGGCGACTGGCTCTGGTA
AGTCTACAACGATGGCGGCAATGACTGGGTATCGTAACCGTCATTCTTCTGGACATATTTTAACGGTAGAAGATCCAATT
GAGTTTGTGCATGAACATGATCAATGCATTGTGACTCAGCGTGAAGTTGGATTAGATACGCAGAGCTATGAAATCGCACT
AAAAAACTCGTTACGCCAAGCGCCAGATATGATCTTAATTGGTGAGATACGCTCGCAAGAGACCATGGAATACGCGATGA
ACTTTGCCGAAACTGGGCACTTATGTATGGCAACTTTACACGCCAATAATGCCAACCAGGCGTTAGAGCGTATTCTGCAT
TTGGTACCGAAAGAGCAAAAAGAACAATTTTTATTTGATCTCTCAATGAACTTACGTGGTGTAGTAGGGCAGCAATTGGT
TCGTGATAAAGATGGTAGTGGCAGGCATGGGGTTTTTGAGATATTGCTTAATACGCCACGTATTTCTGATTTGATCCGAC
GTGGAGAGTTACATGAGCTTAAAGAAACGATGAAACGCTCACGCGAGTCAGGTATGCAAACATTTGATCAAGCGTTATAC
CAATTAGTGATAGATGGAAAAATAACTGAGCAAGATGCAATGCATCATGCTGATTCCGCGAACGACTTACGTCTCCTTCT
TAAAGGTCAGCAAGGCAGTAGTTATAGCGCGAGTTCATTGAGTGATATTAAGATTGATATGGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(66-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1R4B7P8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

76.63

100

0.766
  pilU Pseudomonas stutzeri DSM 10701

57.143

95.109

0.543
  pilU Acinetobacter baylyi ADP1

53.276

95.38

0.508
  pilT Legionella pneumophila strain ERS1305867

41.791

91.033

0.38
  pilT Legionella pneumophila strain Lp02

41.791

91.033

0.38
  pilT Pseudomonas aeruginosa PAK

40.413

92.12

0.372
  pilT Acinetobacter baylyi ADP1

40.606

89.674

0.364
  pilT Pseudomonas stutzeri DSM 10701

39.233

92.12

0.361
  pilT Acinetobacter baumannii D1279779

39.701

91.033

0.361
  pilT Acinetobacter baumannii strain A118

39.701

91.033

0.361
  pilT Acinetobacter nosocomialis M2

39.701

91.033

0.361

Multiple sequence alignment