Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OCU38_RS10725 Genome accession   NZ_AP024885
Coordinates   2319888..2320934 (-) Length   348 a.a.
NCBI ID   WP_152819419.1    Uniprot ID   A0A6I1QCH7
Organism   Vibrio neonatus strain JCM 21521     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2314888..2325934
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCU38_RS10695 - 2315214..2315909 (+) 696 WP_152819422.1 endonuclease -
  OCU38_RS10700 rsmE 2316005..2316736 (+) 732 WP_152819421.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OCU38_RS10705 gshB 2316746..2317693 (+) 948 WP_023405076.1 glutathione synthase -
  OCU38_RS10710 - 2317756..2318316 (+) 561 WP_023405075.1 YqgE/AlgH family protein -
  OCU38_RS10715 ruvX 2318313..2318738 (+) 426 WP_023405074.1 Holliday junction resolvase RuvX -
  OCU38_RS10720 pilU 2318757..2319860 (-) 1104 WP_023405073.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCU38_RS10725 pilT 2319888..2320934 (-) 1047 WP_152819419.1 type IV pilus twitching motility protein PilT Machinery gene
  OCU38_RS10730 - 2320959..2321669 (+) 711 WP_023405071.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCU38_RS10735 proC 2321699..2322523 (+) 825 WP_261823062.1 pyrroline-5-carboxylate reductase -
  OCU38_RS10740 - 2322541..2323110 (+) 570 WP_152819416.1 YggT family protein -
  OCU38_RS10745 - 2323137..2323580 (+) 444 WP_261823063.1 DUF4426 domain-containing protein -
  OCU38_RS10750 - 2323586..2324188 (+) 603 WP_023405067.1 XTP/dITP diphosphatase -
  OCU38_RS10755 hemW 2324185..2325369 (+) 1185 WP_261823064.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38720.18 Da        Isoelectric Point: 5.7353

>NTDB_id=89118 OCU38_RS10725 WP_152819419.1 2319888..2320934(-) (pilT) [Vibrio neonatus strain JCM 21521]
MDITELLRFSVKHNASDLHLSSGLPPMVRIDGDVRRLEMPEFSETEVHELIFDIMDDAQRSEFESNLELDFSIELAGVGR
FRVNAFNQSRGASAVFRTIPTDIPTLEQLDCPPIFENIVNYEKGLVLVTGPTGSGKSTTLAAMVDYINRHHNKHILTIED
PIEFVHQSNKCLINQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGDKNMVRSMLSESLRAVIAQKLLKRVGGGRIACHEIMLANPAIRNLIREDKVAQMYSIIQTGASHGMKTMEQAA
NQLMQQNQVTREEVETKIEIQVGLGGGF

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=89118 OCU38_RS10725 WP_152819419.1 2319888..2320934(-) (pilT) [Vibrio neonatus strain JCM 21521]
ATGGATATCACCGAATTACTTCGCTTTAGTGTAAAGCATAATGCTTCAGATCTACATCTTTCATCGGGCTTACCTCCTAT
GGTGAGGATAGACGGCGATGTGCGACGTCTGGAGATGCCAGAGTTTAGCGAGACTGAAGTCCACGAACTTATTTTTGATA
TCATGGATGATGCCCAACGAAGTGAGTTTGAGTCAAATCTAGAGTTGGATTTTTCGATCGAGCTTGCAGGCGTCGGGCGA
TTTAGGGTTAATGCCTTTAATCAAAGTCGCGGCGCTTCAGCGGTGTTTAGAACCATCCCTACAGATATCCCTACGCTTGA
ACAGCTAGATTGCCCGCCTATCTTCGAAAACATCGTCAATTATGAAAAAGGCTTAGTCTTGGTCACCGGCCCGACAGGAT
CGGGTAAATCTACCACGCTCGCGGCGATGGTGGACTATATTAATCGTCATCACAATAAACATATCTTGACCATTGAAGAT
CCGATTGAATTTGTACACCAAAGTAATAAGTGTTTGATCAATCAGCGTGAAGTACATCGTGATACGCACAGCTTCAAAGC
GGCGCTCCGCTCAGCACTGCGTGAAGATCCAGATGTGATTTTGGTGGGAGAGTTGCGCGACCAAGAGACCATTAGTTTAG
CGCTAACGGCCGCTGAAACAGGGCACTTGGTATTTGGCACATTGCACACCAGTTCGGCAGCCAAAACCATAGATAGAATT
ATTGATGTGTTTCCTGCCGGAGATAAAAACATGGTGCGCTCTATGCTTTCTGAATCACTGCGAGCGGTGATTGCGCAGAA
GCTATTAAAGAGAGTGGGCGGCGGTCGTATTGCTTGTCATGAAATCATGTTGGCTAACCCAGCAATTAGAAATCTAATTC
GGGAAGATAAAGTGGCGCAGATGTATTCCATTATCCAAACGGGCGCGTCACACGGCATGAAAACGATGGAGCAAGCGGCA
AATCAATTGATGCAACAAAATCAGGTCACTAGAGAAGAAGTTGAAACTAAAATTGAGATTCAAGTCGGTCTTGGTGGCGG
TTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I1QCH7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

82.456

98.276

0.81
  pilT Vibrio cholerae strain A1552

82.456

98.276

0.81
  pilT Pseudomonas stutzeri DSM 10701

69.139

96.839

0.67
  pilT Acinetobacter baylyi ADP1

70.859

93.678

0.664
  pilT Acinetobacter baumannii strain A118

68.75

96.552

0.664
  pilT Acinetobacter nosocomialis M2

68.75

96.552

0.664
  pilT Acinetobacter baumannii D1279779

68.75

96.552

0.664
  pilT Pseudomonas aeruginosa PAK

67.647

97.701

0.661
  pilT Legionella pneumophila strain Lp02

66.767

95.115

0.635
  pilT Legionella pneumophila strain ERS1305867

66.767

95.115

0.635
  pilT Neisseria meningitidis 8013

64.565

95.69

0.618
  pilT Neisseria gonorrhoeae MS11

64.264

95.69

0.615
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.599

95.977

0.486
  pilU Pseudomonas stutzeri DSM 10701

41.916

95.977

0.402
  pilU Vibrio cholerae strain A1552

41.317

95.977

0.397
  pilU Acinetobacter baylyi ADP1

38.855

95.402

0.371

Multiple sequence alignment