Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OCU38_RS10720 Genome accession   NZ_AP024885
Coordinates   2318757..2319860 (-) Length   367 a.a.
NCBI ID   WP_023405073.1    Uniprot ID   V5FG76
Organism   Vibrio neonatus strain JCM 21521     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2313757..2324860
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCU38_RS10690 - 2314606..2315100 (+) 495 WP_261823061.1 SprT family zinc-dependent metalloprotease -
  OCU38_RS10695 - 2315214..2315909 (+) 696 WP_152819422.1 endonuclease -
  OCU38_RS10700 rsmE 2316005..2316736 (+) 732 WP_152819421.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OCU38_RS10705 gshB 2316746..2317693 (+) 948 WP_023405076.1 glutathione synthase -
  OCU38_RS10710 - 2317756..2318316 (+) 561 WP_023405075.1 YqgE/AlgH family protein -
  OCU38_RS10715 ruvX 2318313..2318738 (+) 426 WP_023405074.1 Holliday junction resolvase RuvX -
  OCU38_RS10720 pilU 2318757..2319860 (-) 1104 WP_023405073.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCU38_RS10725 pilT 2319888..2320934 (-) 1047 WP_152819419.1 type IV pilus twitching motility protein PilT Machinery gene
  OCU38_RS10730 - 2320959..2321669 (+) 711 WP_023405071.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCU38_RS10735 proC 2321699..2322523 (+) 825 WP_261823062.1 pyrroline-5-carboxylate reductase -
  OCU38_RS10740 - 2322541..2323110 (+) 570 WP_152819416.1 YggT family protein -
  OCU38_RS10745 - 2323137..2323580 (+) 444 WP_261823063.1 DUF4426 domain-containing protein -
  OCU38_RS10750 - 2323586..2324188 (+) 603 WP_023405067.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 367 a.a.        Molecular weight: 40967.75 Da        Isoelectric Point: 6.3417

>NTDB_id=89117 OCU38_RS10720 WP_023405073.1 2318757..2319860(-) (pilU) [Vibrio neonatus strain JCM 21521]
MELKELLGAMTQHKASDLYITVDAPCMFRVDGELQPFGEKLHQSGVTAFLDSIMDNDRRKEYRACREANFAIESESGRFR
VSAFFQRDLPGAVIRHIETEIPSFEDLGLPDTLKDLAMVKRGLVLVVGATGSGKSTTLAAMVGHRNQHQHGHILTVEDPI
EFVHQHQQSIVTQREVGLDTESYEIALKNSLRQAPDMIVIGEIRTPETMRYAMTFAETGHLCVATLHANNANQALERILH
LVSKDQREHFLLDLSLNLKGIVGQQLLKAKDGKGRHVALEILLSTPRSSDLIRQGEIAEIKNYMSKTRQEGICTFDHSLF
NLVMQDKITAEEALRSADSANDLRLMLKNQQGTKGSSPLDDVKIDMG

Nucleotide


Download         Length: 1104 bp        

>NTDB_id=89117 OCU38_RS10720 WP_023405073.1 2318757..2319860(-) (pilU) [Vibrio neonatus strain JCM 21521]
ATGGAATTGAAAGAGTTGTTAGGTGCAATGACACAGCACAAAGCGTCTGATCTTTACATTACAGTCGATGCACCTTGTAT
GTTTCGGGTCGATGGAGAGTTGCAACCGTTTGGTGAAAAGCTGCATCAATCGGGTGTAACGGCATTTTTAGACAGCATTA
TGGATAACGATAGGCGCAAAGAGTATCGAGCCTGTCGAGAAGCCAATTTTGCCATTGAAAGTGAATCGGGGCGTTTTCGT
GTCAGCGCCTTTTTTCAGCGTGATTTACCCGGCGCGGTTATTCGACATATTGAGACAGAAATTCCGTCCTTTGAAGATCT
TGGCTTACCCGATACCTTAAAAGATCTGGCTATGGTTAAGCGAGGTTTAGTGCTGGTGGTCGGAGCAACAGGCTCTGGTA
AGTCGACAACGCTTGCCGCTATGGTGGGGCATAGAAATCAGCATCAACATGGGCATATTTTAACGGTAGAAGATCCGATT
GAATTTGTGCATCAGCATCAGCAAAGCATAGTCACTCAGCGTGAAGTGGGTTTAGATACTGAAAGCTACGAAATCGCGCT
GAAAAACTCGCTGCGACAAGCGCCCGATATGATAGTGATTGGCGAGATCCGCACCCCAGAAACCATGCGTTATGCGATGA
CTTTTGCGGAAACGGGGCACTTGTGTGTGGCAACCTTGCACGCAAATAATGCCAACCAAGCCTTAGAGCGGATTTTACAT
TTAGTCTCAAAAGATCAAAGAGAGCATTTTTTGTTGGATCTTTCGCTTAATTTAAAAGGGATTGTTGGCCAGCAACTGCT
AAAAGCCAAAGATGGAAAAGGGCGTCATGTGGCATTGGAAATACTGCTTTCTACGCCAAGATCGTCCGATCTTATCCGAC
AAGGTGAGATTGCTGAAATAAAGAATTACATGAGTAAAACAAGGCAAGAGGGGATCTGCACTTTTGATCACTCTTTGTTT
AACTTAGTGATGCAAGACAAGATCACTGCAGAAGAAGCGTTGCGCAGTGCAGATTCAGCCAACGATCTGCGTTTAATGCT
TAAAAATCAGCAAGGGACTAAAGGTAGTAGTCCCTTAGATGACGTTAAGATTGATATGGGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB V5FG76

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

68.937

100

0.689
  pilU Pseudomonas stutzeri DSM 10701

55.182

97.275

0.537
  pilU Acinetobacter baylyi ADP1

53.022

99.183

0.526
  pilT Legionella pneumophila strain Lp02

44.675

92.098

0.411
  pilT Legionella pneumophila strain ERS1305867

44.675

92.098

0.411
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.556

98.093

0.398
  pilT Acinetobacter baylyi ADP1

40.407

93.733

0.379
  pilT Acinetobacter nosocomialis M2

40.413

92.371

0.373
  pilT Acinetobacter baumannii D1279779

40.413

92.371

0.373
  pilT Acinetobacter baumannii strain A118

40.413

92.371

0.373
  pilT Neisseria gonorrhoeae MS11

39.71

94.005

0.373
  pilT Neisseria meningitidis 8013

39.71

94.005

0.373
  pilT Pseudomonas aeruginosa PAK

39.823

92.371

0.368
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.419

91.008

0.368
  pilT Vibrio cholerae strain A1552

40.419

91.008

0.368
  pilT Pseudomonas stutzeri DSM 10701

39.233

92.371

0.362

Multiple sequence alignment