Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MHOCP_RS07790 Genome accession   NZ_CP136421
Coordinates   1483903..1484922 (-) Length   339 a.a.
NCBI ID   WP_106006108.1    Uniprot ID   A0A2T0AMF3
Organism   Moorella humiferrea strain OCP     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1478903..1489922
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHOCP_RS07760 (MHOCP_15460) - 1478914..1479312 (-) 399 WP_338823324.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MHOCP_RS07765 (MHOCP_15470) - 1479309..1479788 (-) 480 WP_338823326.1 type II secretion system protein -
  MHOCP_RS07770 (MHOCP_15480) - 1479835..1480578 (-) 744 WP_338823327.1 prepilin peptidase -
  MHOCP_RS07775 (MHOCP_15500) - 1480826..1481611 (-) 786 WP_338823328.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MHOCP_RS07780 (MHOCP_15510) - 1481718..1482254 (-) 537 WP_338823329.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MHOCP_RS07785 (MHOCP_15520) - 1482500..1483708 (-) 1209 WP_338823331.1 type II secretion system F family protein -
  MHOCP_RS07790 (MHOCP_15530) pilT 1483903..1484922 (-) 1020 WP_106006108.1 type IV pilus twitching motility protein PilT Machinery gene
  MHOCP_RS07795 (MHOCP_15540) pilF 1485090..1486742 (-) 1653 WP_338823332.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  MHOCP_RS07800 (MHOCP_15550) aroB 1486806..1487879 (-) 1074 WP_338823333.1 3-dehydroquinate synthase -
  MHOCP_RS07805 (MHOCP_15560) - 1487858..1488379 (-) 522 WP_338823334.1 shikimate kinase -
  MHOCP_RS07810 (MHOCP_15570) aroC 1488475..1489626 (-) 1152 WP_338823336.1 chorismate synthase -

Sequence

Protein


Download         Length: 339 a.a.        Molecular weight: 37121.65 Da        Isoelectric Point: 7.0817

>NTDB_id=890906 MHOCP_RS07790 WP_106006108.1 1483903..1484922(-) (pilT) [Moorella humiferrea strain OCP]
MFDIETILRAAAAAGASDVHLSVGLPPVFRVHGELQVQRQWEPLDAGMTAALVRPMVGERWEVLQERGEIDLAYSLPGVS
RFRVNVFHQRGSVSAAIRLIPREIPSLETLGLPPVVAELAERQHGLVLVTGPTGSGKSTTLAAMVDKINRERSCHIITLE
DPIEYLHQHRRSIVNQREVGSDTRSFASALRAALRQDPDVILVGEMRDLETIATAITAAETGHLVLATLHTSSAIQSVDR
IIDVFPPHQQGQVRIQLADTLEGVITQQLLPRADRKGRVAAVEVLIATPAVKNLIREGKTHQIVSSMQTGARYGMQTMDM
ALRQLVTRGVITDRELNNG

Nucleotide


Download         Length: 1020 bp        

>NTDB_id=890906 MHOCP_RS07790 WP_106006108.1 1483903..1484922(-) (pilT) [Moorella humiferrea strain OCP]
ATGTTCGACATTGAAACCATCCTACGGGCGGCGGCTGCCGCCGGGGCCTCCGATGTCCATCTCTCTGTTGGCCTGCCGCC
GGTTTTCCGGGTCCACGGCGAACTCCAGGTCCAGCGCCAGTGGGAGCCCCTGGATGCCGGGATGACGGCCGCCCTGGTAC
GGCCTATGGTGGGCGAGAGGTGGGAGGTATTGCAAGAGCGGGGAGAAATAGACCTGGCTTACTCCCTGCCCGGCGTAAGC
CGTTTCCGGGTCAATGTCTTTCACCAGCGGGGGAGCGTTAGCGCAGCCATCCGCCTTATACCCAGGGAAATACCCAGCCT
GGAAACCCTGGGATTACCGCCGGTGGTGGCCGAGCTGGCGGAACGACAGCACGGCCTAGTCCTGGTGACGGGGCCGACGG
GTAGCGGCAAGTCCACCACCCTGGCGGCCATGGTGGATAAAATCAACCGGGAACGCAGCTGCCATATTATTACCCTGGAA
GACCCCATCGAATACCTGCACCAGCACCGCCGTAGCATCGTCAACCAGCGGGAAGTGGGTTCAGATACCCGTTCCTTTGC
CAGTGCCCTGCGGGCCGCCCTGCGTCAGGACCCGGATGTTATCCTGGTCGGCGAGATGCGCGATCTGGAGACCATTGCCA
CGGCCATCACGGCCGCGGAAACGGGCCACCTGGTCCTGGCGACTTTACATACCAGCAGTGCCATCCAGAGCGTGGATCGG
ATCATTGACGTTTTCCCGCCCCACCAGCAGGGACAGGTCCGTATCCAGCTGGCTGACACCCTGGAAGGGGTTATCACCCA
GCAGCTTTTACCTCGGGCCGACAGGAAGGGTCGGGTGGCCGCCGTGGAAGTGCTGATTGCTACCCCGGCTGTCAAAAATC
TGATCCGCGAGGGTAAAACCCACCAGATTGTTTCCAGCATGCAGACGGGAGCCCGCTATGGTATGCAGACCATGGACATG
GCTCTGCGGCAGCTGGTCACCAGAGGGGTAATAACGGATAGAGAATTAAACAATGGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2T0AMF3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

58.912

97.64

0.575
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.471

100

0.516
  pilT Vibrio cholerae strain A1552

51.471

100

0.516
  pilT Acinetobacter baylyi ADP1

50.904

97.935

0.499
  pilT Acinetobacter baumannii D1279779

50.751

98.23

0.499
  pilT Acinetobacter baumannii strain A118

50.751

98.23

0.499
  pilT Acinetobacter nosocomialis M2

50.751

98.23

0.499
  pilT Neisseria meningitidis 8013

51.22

96.755

0.496
  pilT Pseudomonas stutzeri DSM 10701

50.602

97.935

0.496
  pilT Neisseria gonorrhoeae MS11

50.915

96.755

0.493
  pilT Pseudomonas aeruginosa PAK

50.151

97.64

0.49
  pilT Legionella pneumophila strain ERS1305867

49.849

97.64

0.487
  pilT Legionella pneumophila strain Lp02

49.849

97.64

0.487
  pilU Pseudomonas stutzeri DSM 10701

45.783

97.935

0.448
  pilU Vibrio cholerae strain A1552

45.483

94.69

0.431
  pilU Acinetobacter baylyi ADP1

42.331

96.165

0.407