Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   MHOCP_RS07795 Genome accession   NZ_CP136421
Coordinates   1485090..1486742 (-) Length   550 a.a.
NCBI ID   WP_338823332.1    Uniprot ID   -
Organism   Moorella humiferrea strain OCP     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1480090..1491742
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHOCP_RS07775 (MHOCP_15500) - 1480826..1481611 (-) 786 WP_338823328.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MHOCP_RS07780 (MHOCP_15510) - 1481718..1482254 (-) 537 WP_338823329.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MHOCP_RS07785 (MHOCP_15520) - 1482500..1483708 (-) 1209 WP_338823331.1 type II secretion system F family protein -
  MHOCP_RS07790 (MHOCP_15530) pilT 1483903..1484922 (-) 1020 WP_106006108.1 type IV pilus twitching motility protein PilT Machinery gene
  MHOCP_RS07795 (MHOCP_15540) pilF 1485090..1486742 (-) 1653 WP_338823332.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  MHOCP_RS07800 (MHOCP_15550) aroB 1486806..1487879 (-) 1074 WP_338823333.1 3-dehydroquinate synthase -
  MHOCP_RS07805 (MHOCP_15560) - 1487858..1488379 (-) 522 WP_338823334.1 shikimate kinase -
  MHOCP_RS07810 (MHOCP_15570) aroC 1488475..1489626 (-) 1152 WP_338823336.1 chorismate synthase -
  MHOCP_RS07815 (MHOCP_15580) - 1489740..1490612 (-) 873 WP_338823337.1 shikimate dehydrogenase -
  MHOCP_RS07820 (MHOCP_15590) - 1490662..1491171 (-) 510 WP_338823339.1 YqeG family HAD IIIA-type phosphatase -

Sequence

Protein


Download         Length: 550 a.a.        Molecular weight: 60378.24 Da        Isoelectric Point: 6.0642

>NTDB_id=890907 MHOCP_RS07795 WP_338823332.1 1485090..1486742(-) (pilF) [Moorella humiferrea strain OCP]
MDTRRRLGDLLIAAGMITPAQLEEALREQKRTGERLGRVLVRLGFITEESILEVLEFQLGIPKVVLVDYHLDPEVVRLVP
EGLARRYQAIPIRLDGNRLLVAMADPLNLLALDDLRLATGKEIMPAIAAEREIEEALSRFWQKETAASMSEAAATALEAP
GPGGTEGAPAVRLVNSFIQQAIQMRASDIHIEPQEGEVRVRLRVDGLLRELTRLPLGVLSSLISRIKIMAGMDIAEKRLP
QDGRFQFTLGKRNVDLRVSSLPTVYGEKIVLRLLDQEAMLLSLDDLGFLPAIKERFENLIHSSYGMLLITGPTGSGKTTT
LYATLNILSSPEKNIITIEDPVEYLLPGINQVRVNPKAGLTFASGLRSILRQDPDIIMVGEIRDRETADIAVRAATTGHL
VFSTLHTNDAAGAVTRLLDMGVEPYLVNSSLIGVVAQRLVRRLCPHCREIYRPEPGSLEKTWLPAVEELWRGRGCQRCSF
TGYAGRTAIQEVLVMNEELRGLVTAKAPAMALKEAAVAAGMVPLIEDGLEKVRQGITTVSEVLRVSLGGL

Nucleotide


Download         Length: 1653 bp        

>NTDB_id=890907 MHOCP_RS07795 WP_338823332.1 1485090..1486742(-) (pilF) [Moorella humiferrea strain OCP]
ATGGATACCCGGCGGCGGCTGGGGGACCTTTTAATTGCGGCCGGGATGATCACCCCGGCCCAGCTGGAGGAGGCCCTGCG
GGAACAAAAGCGTACCGGCGAACGCCTGGGCAGGGTACTGGTGCGGCTGGGTTTCATCACCGAAGAGAGCATCCTGGAAG
TGCTGGAATTTCAGCTGGGCATACCCAAAGTCGTCCTGGTCGACTACCACCTGGACCCGGAGGTGGTGCGTCTGGTGCCG
GAGGGCTTGGCGCGGCGCTACCAGGCCATTCCCATTCGCCTGGACGGTAACCGTCTCCTGGTAGCCATGGCCGACCCCTT
GAACCTGCTGGCCCTGGACGACCTGCGCCTGGCTACCGGCAAGGAGATCATGCCGGCTATAGCTGCTGAAAGGGAAATCG
AAGAGGCTTTGAGCAGGTTCTGGCAGAAGGAAACTGCTGCCAGCATGAGCGAGGCGGCGGCTACTGCCCTGGAAGCTCCC
GGTCCCGGCGGTACCGAAGGGGCACCGGCTGTACGGCTGGTTAACAGCTTTATCCAGCAGGCAATCCAGATGCGGGCCAG
CGATATCCACATCGAACCCCAGGAGGGGGAGGTGCGTGTACGCCTGCGGGTGGATGGCCTGCTGCGGGAGTTAACCCGCC
TGCCCCTGGGGGTTCTAAGCAGCCTGATCTCCAGGATCAAGATCATGGCGGGCATGGATATTGCCGAAAAGCGCCTTCCC
CAGGACGGTCGATTTCAGTTTACCCTGGGTAAACGCAACGTCGACCTGCGGGTTTCCAGCCTGCCCACAGTTTACGGGGA
AAAGATCGTTCTGCGCTTGCTGGACCAGGAGGCCATGCTCCTCTCCCTGGACGACCTGGGATTTTTGCCGGCCATAAAAG
AACGCTTTGAGAATCTCATCCACAGTTCCTACGGCATGCTCCTCATTACCGGTCCCACTGGTAGCGGTAAAACGACGACC
CTCTATGCCACCCTTAACATTTTAAGCTCGCCGGAAAAAAACATCATTACCATTGAAGACCCGGTAGAATACCTGCTGCC
CGGCATCAACCAGGTGCGGGTGAATCCCAAGGCAGGCTTGACCTTTGCCTCCGGGCTGCGGTCTATCCTGCGCCAGGATC
CCGATATCATTATGGTAGGAGAAATTCGCGACCGGGAAACGGCAGACATCGCCGTCCGGGCGGCGACCACCGGCCATCTG
GTGTTCAGCACCCTTCATACCAATGACGCTGCCGGGGCCGTCACCCGCCTCTTAGATATGGGCGTTGAACCTTACCTGGT
CAACTCTTCCCTTATCGGCGTAGTGGCCCAGCGGCTGGTACGCCGGCTCTGCCCCCACTGCCGGGAGATTTACCGACCCG
AACCGGGGTCTTTGGAAAAGACCTGGCTGCCAGCAGTAGAAGAGCTCTGGCGGGGTCGGGGTTGCCAGCGCTGCAGCTTT
ACCGGTTATGCTGGCCGGACGGCCATCCAGGAGGTACTGGTGATGAATGAAGAACTCCGCGGCCTGGTGACCGCCAAGGC
ACCGGCCATGGCCCTGAAAGAGGCGGCCGTGGCTGCCGGTATGGTGCCTTTAATCGAGGACGGCTTGGAAAAAGTCCGCC
AGGGGATTACCACGGTGAGTGAAGTCCTGCGTGTTTCCCTGGGCGGTTTGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-144)

Type II/IV secretion system protein (T2SSE)

(177-442)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

50.091

99.818

0.5
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.372

100

0.455
  pilB Vibrio cholerae strain A1552

42.599

100

0.429
  pilB/pilB1 Synechocystis sp. PCC 6803

38.424

100

0.425
  pilB Vibrio parahaemolyticus RIMD 2210633

41.577

100

0.422
  pilB Vibrio campbellii strain DS40M4

41.472

100

0.42
  pilB Acinetobacter baylyi ADP1

41.367

100

0.418
  pilB Acinetobacter baumannii D1279779

40.253

100

0.405
  pilF Neisseria gonorrhoeae MS11

39.383

100

0.395
  pilB Legionella pneumophila strain ERS1305867

40.15

96.909

0.389