Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MTBGP_RS08985 Genome accession   NZ_CP136416
Coordinates   1736357..1737376 (-) Length   339 a.a.
NCBI ID   WP_338817897.1    Uniprot ID   -
Organism   Moorella thermoacetica strain BGP     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1731357..1742376
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MTBGP_RS08955 (MTBGP_17900) - 1731751..1732251 (-) 501 WP_338817891.1 hypothetical protein -
  MTBGP_RS08960 (MTBGP_17910) - 1732248..1732646 (-) 399 WP_338817892.1 type II secretion system protein -
  MTBGP_RS08965 (MTBGP_17920) - 1732643..1733164 (-) 522 WP_338817893.1 type II secretion system protein -
  MTBGP_RS08970 (MTBGP_17930) - 1733196..1733939 (-) 744 WP_338825737.1 prepilin peptidase -
  MTBGP_RS08975 (MTBGP_17940) - 1734169..1734699 (-) 531 WP_338817895.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MTBGP_RS08980 (MTBGP_17950) - 1734949..1736157 (-) 1209 WP_338817896.1 type II secretion system F family protein -
  MTBGP_RS08985 (MTBGP_17960) pilT 1736357..1737376 (-) 1020 WP_338817897.1 type IV pilus twitching motility protein PilT Machinery gene
  MTBGP_RS08990 (MTBGP_17970) pilF 1737390..1739051 (-) 1662 WP_338817898.1 GspE/PulE family protein Machinery gene
  MTBGP_RS08995 (MTBGP_17980) aroB 1739057..1740151 (-) 1095 WP_338817899.1 3-dehydroquinate synthase -
  MTBGP_RS09000 (MTBGP_17990) - 1740130..1740651 (-) 522 WP_338817900.1 shikimate kinase -
  MTBGP_RS09005 (MTBGP_18000) aroC 1740705..1741856 (-) 1152 WP_338817901.1 chorismate synthase -

Sequence

Protein


Download         Length: 339 a.a.        Molecular weight: 37028.64 Da        Isoelectric Point: 6.6636

>NTDB_id=890817 MTBGP_RS08985 WP_338817897.1 1736357..1737376(-) (pilT) [Moorella thermoacetica strain BGP]
MLDIETILVAAAAAGASDVHISAGLPPVFRVNGELQVQRQWDPLDLEMTARLVRPVVGDKWEVFQEQGEIDLAYSLPGVS
RFRVNVFHQRGSVGAAIRLIPREIPSLESLGLPPVVAELACRQHGLILVTGPTGSGKSTTLAAMVDKINRERSCHIITLE
DPIEYLHQHRRSIVNQREVGSDTRSFASALRAALRQDPDVILVGEMRDLETIATAITAAETGHLVLATLHTSSAVQSVDR
IIDVFPPHQQGQVRIQLADTLEGVITQQLLPRADRKGRVAAVEVLIATPAVKNLIREGKTHQIVSTMQTGARYGMQTMEM
ALRQLITRGVIFEEGLNIG

Nucleotide


Download         Length: 1020 bp        

>NTDB_id=890817 MTBGP_RS08985 WP_338817897.1 1736357..1737376(-) (pilT) [Moorella thermoacetica strain BGP]
ATGCTCGATATCGAAACAATCCTGGTAGCGGCTGCCGCCGCCGGTGCCTCCGACGTCCATATCTCTGCCGGGCTGCCGCC
GGTTTTCCGGGTGAACGGCGAACTCCAGGTCCAGCGCCAGTGGGACCCCCTGGATTTGGAGATGACAGCCCGCCTGGTAC
GGCCTGTAGTGGGCGATAAGTGGGAGGTATTTCAGGAGCAAGGTGAAATCGATCTGGCTTACTCTCTGCCCGGTGTAAGC
CGTTTCCGGGTCAATGTCTTTCACCAGCGGGGGAGCGTCGGGGCGGCCATCCGCCTTATCCCCAGGGAGATCCCCAGCCT
GGAGTCCCTGGGTTTACCGCCGGTGGTGGCCGAACTGGCGTGCAGACAGCACGGCCTGATCCTGGTGACGGGTCCGACTG
GCAGCGGTAAATCCACCACCCTGGCGGCCATGGTGGATAAAATCAACCGGGAGCGGAGCTGTCACATCATCACCCTGGAA
GACCCCATTGAGTACTTGCACCAGCACCGCCGTAGCATAGTCAACCAGCGGGAAGTGGGTTCTGATACCAGATCCTTTGC
CAGCGCCCTGCGGGCCGCCCTGCGCCAGGACCCCGACGTTATCCTGGTCGGGGAGATGCGCGACCTGGAAACCATCGCCA
CGGCCATTACCGCTGCCGAAACAGGTCACCTGGTCCTGGCGACTTTGCACACCAGCAGTGCCGTCCAGAGCGTGGACCGG
ATCATTGACGTTTTCCCGCCCCACCAGCAGGGCCAGGTACGAATCCAGCTTGCCGACACCCTGGAAGGGGTGATCACCCA
GCAGCTTTTACCGCGGGCCGACAGGAAGGGGCGGGTGGCGGCCGTAGAAGTGTTGATAGCCACCCCGGCAGTGAAGAATC
TCATCCGCGAGGGTAAAACCCATCAAATCGTTTCCACTATGCAGACCGGAGCCCGCTACGGGATGCAGACCATGGAGATG
GCGCTGCGGCAACTGATCACCCGGGGAGTAATTTTTGAGGAAGGTTTAAATATTGGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

56.38

99.41

0.56
  pilT Vibrio cholerae strain A1552

51.807

97.935

0.507
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.807

97.935

0.507
  pilT Acinetobacter baumannii D1279779

51.205

97.935

0.501
  pilT Acinetobacter baumannii strain A118

51.205

97.935

0.501
  pilT Acinetobacter nosocomialis M2

51.205

97.935

0.501
  pilT Acinetobacter baylyi ADP1

50.904

97.935

0.499
  pilT Pseudomonas aeruginosa PAK

49.851

98.82

0.493
  pilT Pseudomonas stutzeri DSM 10701

50.301

97.935

0.493
  pilT Neisseria meningitidis 8013

49.401

98.525

0.487
  pilT Neisseria gonorrhoeae MS11

48.955

98.82

0.484
  pilT Legionella pneumophila strain Lp02

49.245

97.64

0.481
  pilT Legionella pneumophila strain ERS1305867

49.245

97.64

0.481
  pilU Pseudomonas stutzeri DSM 10701

45.858

99.705

0.457
  pilU Vibrio cholerae strain A1552

45

94.395

0.425
  pilU Acinetobacter baylyi ADP1

43.558

96.165

0.419