Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   Pcarn_RS11700 Genome accession   NZ_AP024881
Coordinates   2557360..2558400 (-) Length   346 a.a.
NCBI ID   WP_261834041.1    Uniprot ID   -
Organism   Vibrio ishigakensis strain C1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2552360..2563400
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Pcarn_RS11670 - 2552758..2553459 (+) 702 WP_261834035.1 endonuclease -
  Pcarn_RS11675 rsmE 2553528..2554259 (+) 732 WP_261834036.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  Pcarn_RS11680 gshB 2554269..2555216 (+) 948 WP_261834037.1 glutathione synthase -
  Pcarn_RS11685 - 2555269..2555829 (+) 561 WP_261834038.1 YqgE/AlgH family protein -
  Pcarn_RS11690 ruvX 2555826..2556251 (+) 426 WP_261834039.1 Holliday junction resolvase RuvX -
  Pcarn_RS11695 pilU 2556248..2557351 (-) 1104 WP_261834040.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Pcarn_RS11700 pilT 2557360..2558400 (-) 1041 WP_261834041.1 type IV pilus twitching motility protein PilT Machinery gene
  Pcarn_RS11705 - 2558425..2559129 (+) 705 WP_261834042.1 YggS family pyridoxal phosphate-dependent enzyme -
  Pcarn_RS11710 - 2559166..2561325 (-) 2160 WP_261834043.1 TRAP transporter fused permease subunit -
  Pcarn_RS11715 - 2561381..2562337 (-) 957 WP_261834044.1 TAXI family TRAP transporter solute-binding subunit -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38379.95 Da        Isoelectric Point: 6.0521

>NTDB_id=89054 Pcarn_RS11700 WP_261834041.1 2557360..2558400(-) (pilT) [Vibrio ishigakensis strain C1]
MDITELLRFSVKHNASDLHLSSGLPPMVRIDGDVRRLEIDALTENEVHNLVFDIMDDAQRSEFEAKLEIDFSIELQSVGR
FRVNAFQQSRGASAVFRTIPTVIPSLEELETPPIFEKIANYEKGLVLVTGPTGSGKSTTLAAMVDHINKHQNKHVLTIED
PIEFVHHSDKCLINQREVHRDTHSFKAALRSALREDPDVILVGELRDMETISLALTAAETGHLVFGTLHTSSAAKTIDRV
IDVFPAGDKNMVRSMLSESLRAVIAQKLLKRVGGGRVACHEIMLANPAIRNLVREDKVAQMYSVIQTGAAQGMKTMEQSA
RELLQRGVVSVDEVNAKIEQTMGLEF

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=89054 Pcarn_RS11700 WP_261834041.1 2557360..2558400(-) (pilT) [Vibrio ishigakensis strain C1]
ATGGATATCACGGAATTGCTTCGCTTTAGTGTAAAGCATAATGCATCAGATCTACATCTGTCATCGGGTTTACCTCCCAT
GGTGCGAATCGATGGTGATGTACGTCGCTTAGAGATAGATGCGCTGACGGAGAACGAAGTGCATAACCTAGTGTTCGATA
TTATGGATGATGCTCAGCGCAGTGAATTCGAAGCCAAGCTAGAGATAGATTTTTCTATCGAGCTGCAATCGGTAGGGCGC
TTTCGTGTGAATGCCTTCCAGCAAAGCCGTGGTGCGTCGGCCGTGTTCCGAACCATTCCGACCGTTATCCCATCCCTTGA
AGAGCTCGAGACGCCCCCGATCTTTGAAAAGATTGCCAATTATGAGAAGGGCTTAGTCTTGGTCACCGGGCCTACTGGTT
CGGGTAAATCCACCACCTTAGCGGCCATGGTTGACCATATTAACAAGCATCAGAACAAGCATGTGTTGACCATAGAAGAC
CCAATCGAGTTTGTGCATCACAGTGATAAGTGCCTGATAAACCAAAGGGAAGTACATAGAGATACCCACAGTTTTAAGGC
GGCGCTTCGCTCTGCGTTGCGTGAAGACCCAGACGTTATCTTAGTGGGTGAGCTTAGGGATATGGAAACCATAAGCCTTG
CTCTGACTGCGGCTGAAACTGGGCATCTAGTATTCGGTACCTTGCATACGAGCTCAGCGGCTAAGACTATCGATAGGGTT
ATCGATGTCTTTCCTGCTGGAGACAAGAACATGGTGCGCTCAATGCTGTCGGAATCGCTAAGAGCAGTGATAGCACAAAA
GCTATTGAAGCGAGTTGGCGGAGGGCGTGTGGCCTGTCATGAGATCATGCTGGCGAATCCTGCTATTCGTAACCTAGTTC
GCGAAGACAAGGTGGCGCAGATGTATTCCGTGATTCAAACAGGTGCGGCGCAGGGAATGAAAACCATGGAGCAATCGGCG
CGTGAGCTTCTGCAAAGAGGGGTGGTCTCGGTTGATGAGGTCAACGCTAAGATTGAGCAGACCATGGGGTTGGAGTTCTA
G

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

81.711

97.977

0.801
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

81.711

97.977

0.801
  pilT Acinetobacter baumannii D1279779

70.606

95.376

0.673
  pilT Acinetobacter baumannii strain A118

70.606

95.376

0.673
  pilT Acinetobacter nosocomialis M2

70.606

95.376

0.673
  pilT Pseudomonas stutzeri DSM 10701

68.235

98.266

0.671
  pilT Acinetobacter baylyi ADP1

68.452

97.11

0.665
  pilT Pseudomonas aeruginosa PAK

66.469

97.399

0.647
  pilT Legionella pneumophila strain Lp02

66.061

95.376

0.63
  pilT Legionella pneumophila strain ERS1305867

66.061

95.376

0.63
  pilT Neisseria meningitidis 8013

62.248

100

0.624
  pilT Neisseria gonorrhoeae MS11

61.96

100

0.621
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.198

96.532

0.494
  pilU Vibrio cholerae strain A1552

40.923

93.931

0.384
  pilU Pseudomonas stutzeri DSM 10701

39.222

96.532

0.379
  pilU Acinetobacter baylyi ADP1

39.157

95.954

0.376

Multiple sequence alignment