Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   Pcarn_RS11695 Genome accession   NZ_AP024881
Coordinates   2556248..2557351 (-) Length   367 a.a.
NCBI ID   WP_261834040.1    Uniprot ID   -
Organism   Vibrio ishigakensis strain C1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2551248..2562351
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Pcarn_RS11660 - 2551318..2552115 (+) 798 WP_261835678.1 DUF2189 domain-containing protein -
  Pcarn_RS11665 - 2552199..2552693 (+) 495 WP_261834034.1 SprT family zinc-dependent metalloprotease -
  Pcarn_RS11670 - 2552758..2553459 (+) 702 WP_261834035.1 endonuclease -
  Pcarn_RS11675 rsmE 2553528..2554259 (+) 732 WP_261834036.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  Pcarn_RS11680 gshB 2554269..2555216 (+) 948 WP_261834037.1 glutathione synthase -
  Pcarn_RS11685 - 2555269..2555829 (+) 561 WP_261834038.1 YqgE/AlgH family protein -
  Pcarn_RS11690 ruvX 2555826..2556251 (+) 426 WP_261834039.1 Holliday junction resolvase RuvX -
  Pcarn_RS11695 pilU 2556248..2557351 (-) 1104 WP_261834040.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Pcarn_RS11700 pilT 2557360..2558400 (-) 1041 WP_261834041.1 type IV pilus twitching motility protein PilT Machinery gene
  Pcarn_RS11705 - 2558425..2559129 (+) 705 WP_261834042.1 YggS family pyridoxal phosphate-dependent enzyme -
  Pcarn_RS11710 - 2559166..2561325 (-) 2160 WP_261834043.1 TRAP transporter fused permease subunit -
  Pcarn_RS11715 - 2561381..2562337 (-) 957 WP_261834044.1 TAXI family TRAP transporter solute-binding subunit -

Sequence

Protein


Download         Length: 367 a.a.        Molecular weight: 40803.40 Da        Isoelectric Point: 6.0961

>NTDB_id=89053 Pcarn_RS11695 WP_261834040.1 2556248..2557351(-) (pilU) [Vibrio ishigakensis strain C1]
MELKQVLEAMIEQKASDLYITVDSPCMFRIDGELTPFGEKLHQSGVTALLDSIMDNDRRKEYRASRESNFAIESGEGRFR
VSAFFQRDLPGAVIRHIETRIPTMDELGLPQSIKDLSLVKRGLVLVVGATGSGKSTTLAAMVGHRNQTQRGHILTVEDPI
EFVHQHNSSIVTQREVGLDTESYEIALKNSLRQAPDMIVIGEIRTPETMRYAMTFAETGHLCVATLHANNANQALERILH
LVPKEQREHFLLDLSLNLKGIVAQQLLKDKEGKGRHLALEVLLATPRASELVRQGEISEVKNYMSKTNNEGVCTFDNSLF
QLVIDGKVSEEDALRSADSSNELRLKLKNHRGGDSSSSLDTVSIDMG

Nucleotide


Download         Length: 1104 bp        

>NTDB_id=89053 Pcarn_RS11695 WP_261834040.1 2556248..2557351(-) (pilU) [Vibrio ishigakensis strain C1]
ATGGAGCTAAAACAAGTGTTAGAGGCCATGATTGAGCAGAAGGCCTCAGATCTCTATATCACGGTAGATTCACCCTGCAT
GTTCCGCATCGATGGCGAGCTAACCCCATTTGGTGAAAAACTGCATCAATCTGGGGTGACGGCTCTGCTCGATAGCATCA
TGGATAATGACAGGCGCAAAGAGTATCGCGCCTCTCGCGAGTCTAACTTTGCTATCGAATCTGGCGAAGGAAGATTTAGG
GTCAGTGCCTTTTTCCAGCGAGACCTTCCGGGGGCGGTTATTCGTCATATTGAGACGCGAATTCCAACTATGGATGAGCT
AGGCCTGCCACAAAGCATTAAGGATCTTTCGCTAGTTAAACGAGGGCTAGTGTTAGTGGTGGGGGCAACAGGCTCAGGTA
AATCCACCACACTTGCTGCTATGGTTGGGCATAGAAATCAGACTCAGCGAGGGCATATCCTAACCGTTGAAGACCCTATC
GAATTTGTGCATCAACATAACTCGAGCATCGTTACTCAGCGTGAGGTGGGATTAGATACCGAGAGCTATGAGATTGCGCT
TAAAAACTCTCTTCGTCAGGCACCAGATATGATAGTGATAGGCGAGATACGAACGCCTGAGACTATGCGCTATGCCATGA
CCTTTGCAGAAACGGGTCATCTGTGTGTTGCGACCTTGCATGCAAATAATGCTAACCAAGCCCTAGAGCGTATCTTGCAT
TTGGTTCCTAAAGAACAACGAGAGCATTTTTTATTGGATCTTTCGTTGAACCTAAAAGGGATAGTTGCTCAACAGTTACT
CAAGGATAAAGAAGGCAAAGGACGTCACCTTGCGCTTGAGGTACTCCTGGCAACGCCAAGGGCATCAGAGTTGGTGCGTC
AGGGAGAGATCTCCGAGGTGAAGAACTATATGTCTAAGACCAACAATGAGGGAGTTTGCACCTTTGATAACTCTCTATTC
CAGTTGGTTATAGATGGCAAAGTCAGTGAGGAAGATGCACTGAGATCGGCTGACTCTAGCAATGAACTGCGCCTAAAACT
AAAGAATCACCGCGGAGGTGATTCTAGTAGTAGCTTGGATACGGTGAGTATCGATATGGGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

68.665

100

0.687
  pilU Pseudomonas stutzeri DSM 10701

53.714

95.368

0.512
  pilU Acinetobacter baylyi ADP1

52.422

95.64

0.501
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.71

99.728

0.406
  pilT Legionella pneumophila strain Lp02

41.888

92.371

0.387
  pilT Legionella pneumophila strain ERS1305867

41.888

92.371

0.387
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.23

94.823

0.381
  pilT Vibrio cholerae strain A1552

40.23

94.823

0.381
  pilT Pseudomonas aeruginosa PAK

41.003

92.371

0.379
  pilT Acinetobacter baumannii strain A118

40.413

92.371

0.373
  pilT Acinetobacter baumannii D1279779

40.413

92.371

0.373
  pilT Acinetobacter nosocomialis M2

40.413

92.371

0.373
  pilT Acinetobacter baylyi ADP1

39.883

92.916

0.371
  pilT Pseudomonas stutzeri DSM 10701

39.528

92.371

0.365

Multiple sequence alignment