Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RXV79_RS25050 Genome accession   NZ_CP136336
Coordinates   5281380..5282423 (-) Length   347 a.a.
NCBI ID   WP_201809207.1    Uniprot ID   A0A937A3H4
Organism   Piscinibacter gummiphilus strain SBD 7-3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5276380..5287423
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RXV79_RS25020 (RXV79_25020) - 5276832..5277212 (+) 381 WP_316700840.1 YraN family protein -
  RXV79_RS25025 (RXV79_25025) - 5277276..5277872 (+) 597 WP_316700841.1 phosphoheptose isomerase -
  RXV79_RS25030 (RXV79_25030) - 5277869..5278510 (+) 642 WP_316700842.1 BON domain-containing protein -
  RXV79_RS25035 (RXV79_25035) - 5278571..5279464 (-) 894 WP_316700844.1 NAD(P)-dependent oxidoreductase -
  RXV79_RS25040 (RXV79_25040) pilU 5279528..5280664 (-) 1137 WP_316700845.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RXV79_RS25045 (RXV79_25045) - 5280694..5281344 (-) 651 WP_316700846.1 cyclic nucleotide-binding domain-containing protein -
  RXV79_RS25050 (RXV79_25050) pilT 5281380..5282423 (-) 1044 WP_201809207.1 type IV pilus twitching motility protein PilT Machinery gene
  RXV79_RS25055 (RXV79_25055) - 5282508..5283194 (+) 687 WP_316700848.1 YggS family pyridoxal phosphate-dependent enzyme -
  RXV79_RS25060 (RXV79_25060) - 5283294..5286290 (+) 2997 WP_316700849.1 L-glutamate gamma-semialdehyde dehydrogenase -
  RXV79_RS25065 (RXV79_25065) - 5286287..5286775 (-) 489 WP_316700850.1 GFA family protein -
  RXV79_RS25070 (RXV79_25070) - 5286841..5287233 (-) 393 WP_316700851.1 4-oxalocrotonate tautomerase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38246.90 Da        Isoelectric Point: 7.0671

>NTDB_id=890086 RXV79_RS25050 WP_201809207.1 5281380..5282423(-) (pilT) [Piscinibacter gummiphilus strain SBD 7-3]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDPLEHKQVHEMVYDIMNDSQRKAYEETLECDFSFEIQGLAR
FRVNAFNQNRGAGAVFRTIPSKILTLEQLNTPKVFAELALKPRGLVLVTGPTGSGKSTTLAAMVNHLNENEYGHVLTVED
PIEFVHESKKCLVNQREVGPHTLSFSNALRSALREDPDAILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPAAEKEMVRAMLSESLVAVISQTLCKLKDGSGRVAAHEIMLGTAAIRNLIRENKIAQMYSSIQTGNTVGMQTLDQN
LSDLVRRNIIAPAEARNKAKFPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=890086 RXV79_RS25050 WP_201809207.1 5281380..5282423(-) (pilT) [Piscinibacter gummiphilus strain SBD 7-3]
ATGGACATCACCCAACTGTTGGCGTTCTCGGTCAAGAACAAAGCGTCCGACTTGCACCTCTCTGCCGGCCTGCCTCCGAT
GATCCGTGTGCACGGCGACGTGCGGCGCATCAACGTCGACCCGCTCGAGCACAAGCAGGTGCACGAGATGGTCTACGACA
TCATGAACGACTCGCAGCGCAAGGCGTATGAAGAAACGCTCGAGTGCGACTTCTCGTTCGAGATCCAGGGCCTCGCGCGT
TTCCGCGTCAACGCCTTCAACCAGAACCGCGGCGCGGGCGCCGTGTTCCGGACGATTCCCTCGAAGATCCTCACGCTCGA
GCAGCTCAACACGCCCAAGGTATTCGCCGAACTCGCACTCAAGCCACGCGGCCTGGTGCTCGTGACGGGGCCGACGGGCT
CGGGCAAGTCGACCACGCTCGCCGCGATGGTCAACCACCTGAACGAAAACGAATACGGTCACGTGCTGACGGTCGAAGAC
CCGATCGAGTTCGTGCACGAGTCGAAGAAGTGCCTGGTCAACCAGCGTGAGGTCGGCCCGCACACCCTGAGCTTCTCGAA
CGCGCTGCGCTCCGCGCTGCGCGAAGACCCCGACGCCATCCTGGTCGGCGAAATGCGCGACCTGGAGACCATCCGCCTCG
CGCTGACCGCCGCTGAAACGGGCCACCTGGTGTTCGGCACGCTGCACACGAGCTCCGCTGCCAAGACCATCGACCGCGTG
GTCGACGTGTTCCCGGCGGCAGAAAAGGAAATGGTGCGCGCGATGCTCTCCGAGTCGCTCGTCGCCGTGATCTCGCAGAC
CCTGTGCAAGCTGAAGGACGGCAGCGGCCGTGTCGCCGCGCACGAGATCATGTTGGGCACTGCGGCCATCCGAAATCTCA
TCCGCGAAAACAAGATCGCACAGATGTACTCGTCGATCCAGACCGGCAACACGGTGGGCATGCAGACGCTCGACCAGAAC
CTGTCCGACCTCGTCCGCCGCAACATCATTGCGCCGGCCGAAGCGCGCAACAAGGCCAAGTTCCCCGAAAACTTCCCCGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

77.391

99.424

0.769
  pilT Pseudomonas stutzeri DSM 10701

76.232

99.424

0.758
  pilT Acinetobacter baumannii D1279779

75.942

99.424

0.755
  pilT Acinetobacter baumannii strain A118

75.942

99.424

0.755
  pilT Acinetobacter nosocomialis M2

75.942

99.424

0.755
  pilT Acinetobacter baylyi ADP1

73.913

99.424

0.735
  pilT Legionella pneumophila strain Lp02

72.566

97.695

0.709
  pilT Legionella pneumophila strain ERS1305867

72.566

97.695

0.709
  pilT Neisseria meningitidis 8013

67.826

99.424

0.674
  pilT Neisseria gonorrhoeae MS11

67.536

99.424

0.671
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.272

97.406

0.646
  pilT Vibrio cholerae strain A1552

66.272

97.406

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

99.424

0.504
  pilU Vibrio cholerae strain A1552

41.888

97.695

0.409
  pilU Acinetobacter baylyi ADP1

40.173

99.712

0.401
  pilU Pseudomonas stutzeri DSM 10701

42.593

93.372

0.398