Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RXV79_RS25040 Genome accession   NZ_CP136336
Coordinates   5279528..5280664 (-) Length   378 a.a.
NCBI ID   WP_316700845.1    Uniprot ID   -
Organism   Piscinibacter gummiphilus strain SBD 7-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5274528..5285664
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RXV79_RS25010 (RXV79_25010) - 5275569..5275907 (+) 339 WP_316700838.1 hypothetical protein -
  RXV79_RS25015 (RXV79_25015) rsmI 5275928..5276833 (-) 906 WP_316700839.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  RXV79_RS25020 (RXV79_25020) - 5276832..5277212 (+) 381 WP_316700840.1 YraN family protein -
  RXV79_RS25025 (RXV79_25025) - 5277276..5277872 (+) 597 WP_316700841.1 phosphoheptose isomerase -
  RXV79_RS25030 (RXV79_25030) - 5277869..5278510 (+) 642 WP_316700842.1 BON domain-containing protein -
  RXV79_RS25035 (RXV79_25035) - 5278571..5279464 (-) 894 WP_316700844.1 NAD(P)-dependent oxidoreductase -
  RXV79_RS25040 (RXV79_25040) pilU 5279528..5280664 (-) 1137 WP_316700845.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RXV79_RS25045 (RXV79_25045) - 5280694..5281344 (-) 651 WP_316700846.1 cyclic nucleotide-binding domain-containing protein -
  RXV79_RS25050 (RXV79_25050) pilT 5281380..5282423 (-) 1044 WP_201809207.1 type IV pilus twitching motility protein PilT Machinery gene
  RXV79_RS25055 (RXV79_25055) - 5282508..5283194 (+) 687 WP_316700848.1 YggS family pyridoxal phosphate-dependent enzyme -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42325.58 Da        Isoelectric Point: 6.7252

>NTDB_id=890085 RXV79_RS25040 WP_316700845.1 5279528..5280664(-) (pilU) [Piscinibacter gummiphilus strain SBD 7-3]
MERDQASKFVNDLLRLMISRNGSDLFLTADFPPAIKVDGKITKVSPQPLTSQHTLALTRSVMNDKQAAEFEKTKECNFAI
SPQGIGRFRVNAFVQQGHVGLVLRTIPQTLPTIDSLNLPQILKDVTMTKRGLVIFVGATGSGKSTSLAAMIDYRNENSYG
HIITIEDPVEFVHPHKNCIVTQREVGLDTEDWFSALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLKGLISQRLLPRQEGRGRIAAVEILLNTPLISDLIFKGEVAEIKEIMKKSRELG
MQTFDQALFDLYENHLVTYEDALRNADSVNDLRLQIKLHSNRARSSDLSAGTEHLTIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=890085 RXV79_RS25040 WP_316700845.1 5279528..5280664(-) (pilU) [Piscinibacter gummiphilus strain SBD 7-3]
ATGGAAAGAGACCAGGCATCGAAATTCGTCAACGACCTGCTGCGGCTGATGATCTCCCGCAACGGCTCCGACCTCTTCCT
CACGGCCGACTTCCCGCCGGCCATCAAGGTCGACGGCAAGATCACCAAGGTGTCGCCGCAGCCGCTGACGAGCCAGCACA
CGCTGGCCCTCACCCGCTCGGTGATGAACGACAAGCAGGCCGCCGAGTTCGAGAAGACGAAGGAATGCAACTTCGCGATC
TCGCCGCAGGGCATCGGCCGCTTCCGCGTCAACGCCTTCGTGCAGCAGGGCCATGTGGGCCTCGTGCTGCGGACGATTCC
GCAGACGCTCCCCACCATCGACAGCCTCAACCTGCCGCAGATCCTGAAAGACGTGACGATGACCAAGCGCGGGCTGGTTA
TCTTCGTGGGTGCCACCGGCTCCGGCAAGAGCACCTCGCTCGCGGCCATGATCGACTACCGCAACGAGAACTCCTACGGC
CACATCATCACCATCGAAGACCCGGTGGAGTTCGTGCACCCGCACAAGAACTGCATCGTGACGCAGCGCGAGGTCGGCCT
CGACACCGAAGACTGGTTCTCGGCCCTGAAGAACACCCTGCGCCAGGCGCCCGACGTGATCCTGATGGGTGAAATCCGCG
ACCGCGAGACCATGGAGCACGCAGTGGCCTTCGCCGAAACCGGCCACCTGTGCATGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTCGACCGCATCATCAACTTCTTCCCCGAAGAGCGCCGCGCCCAGCTGCTGATGGACCTGTCGCTCAACCT
GAAGGGCCTGATCTCGCAGCGCCTCTTGCCGCGCCAGGAAGGGCGAGGCCGCATTGCCGCGGTCGAGATCCTGCTCAACA
CGCCGTTGATCAGCGACCTGATCTTCAAGGGCGAGGTGGCCGAGATCAAGGAAATCATGAAGAAGAGCCGCGAGCTCGGC
ATGCAGACCTTCGACCAGGCGCTCTTCGACCTCTACGAGAACCACCTCGTGACCTACGAAGACGCACTGCGCAACGCCGA
CTCGGTGAACGACCTGCGCCTGCAGATCAAGCTGCACAGCAACCGGGCGCGCAGCTCCGATCTGTCGGCCGGCACCGAAC
ACCTGACCATTGTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(119-256)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.115

96.825

0.611
  pilU Acinetobacter baylyi ADP1

57.923

96.825

0.561
  pilU Vibrio cholerae strain A1552

53.444

96.032

0.513
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.884

89.153

0.418
  pilT Pseudomonas aeruginosa PAK

44.51

89.153

0.397
  pilT Pseudomonas stutzeri DSM 10701

43.027

89.153

0.384
  pilT Acinetobacter baylyi ADP1

42.478

89.683

0.381
  pilT Acinetobacter baumannii D1279779

43.243

88.095

0.381
  pilT Acinetobacter nosocomialis M2

43.243

88.095

0.381
  pilT Acinetobacter baumannii strain A118

43.243

88.095

0.381
  pilT Legionella pneumophila strain ERS1305867

42.042

88.095

0.37
  pilT Legionella pneumophila strain Lp02

42.042

88.095

0.37
  pilT Vibrio cholerae strain A1552

43.533

83.862

0.365
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.533

83.862

0.365