Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OCU48_RS01925 Genome accession   NZ_AP024878
Coordinates   400856..401902 (+) Length   348 a.a.
NCBI ID   WP_141346877.1    Uniprot ID   A0A4Y3HZ80
Organism   Vibrio inusitatus strain LMG 23434     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 395856..406902
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCU48_RS01895 hemW 396410..397588 (-) 1179 WP_141346883.1 radical SAM family heme chaperone HemW -
  OCU48_RS01900 - 397585..398190 (-) 606 WP_141346882.1 XTP/dITP diphosphatase -
  OCU48_RS01905 - 398195..398638 (-) 444 WP_141346881.1 DUF4426 domain-containing protein -
  OCU48_RS01910 - 398680..399249 (-) 570 WP_141346880.1 YggT family protein -
  OCU48_RS01915 proC 399268..400092 (-) 825 WP_141346879.1 pyrroline-5-carboxylate reductase -
  OCU48_RS01920 - 400121..400831 (-) 711 WP_141346878.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCU48_RS01925 pilT 400856..401902 (+) 1047 WP_141346877.1 type IV pilus twitching motility protein PilT Machinery gene
  OCU48_RS01930 pilU 401932..403035 (+) 1104 WP_141346876.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCU48_RS01935 ruvX 403039..403464 (-) 426 WP_141346875.1 Holliday junction resolvase RuvX -
  OCU48_RS01940 - 403461..404021 (-) 561 WP_141346874.1 YqgE/AlgH family protein -
  OCU48_RS01945 gshB 404089..405036 (-) 948 WP_141346873.1 glutathione synthase -
  OCU48_RS01950 rsmE 405046..405777 (-) 732 WP_141346872.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OCU48_RS01955 - 405872..406567 (-) 696 WP_141346871.1 endonuclease -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38610.10 Da        Isoelectric Point: 5.6264

>NTDB_id=89007 OCU48_RS01925 WP_141346877.1 400856..401902(+) (pilT) [Vibrio inusitatus strain LMG 23434]
MDITELLRFSVKHNASDLHLSSGLPPMVRIDGDVRRLEMPEFSEAEVHELIFDIMDDAQRSEFESNLELDFSIELGGVGR
FRVNAFNQSRGASAVFRTIPTTIPTLEELDCPPIFEKIANYEKGLVLVTGPTGSGKSTTLAAMVDYINRRQNKHILTIED
PIEFVHQSSKCLINQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGDKNMVRSMLSESLRAVIAQKLLKRIGGGRVACHEIMLANPAIRNLVREDKVAQMYSIIQTGAAQGMRTMEQSV
NQLMQQNLVSREEAEAKVEIQTGLGTGF

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=89007 OCU48_RS01925 WP_141346877.1 400856..401902(+) (pilT) [Vibrio inusitatus strain LMG 23434]
ATGGATATCACCGAATTGCTTCGCTTTAGTGTAAAGCATAATGCCTCAGATCTACATCTTTCATCAGGACTACCGCCAAT
GGTAAGGATTGATGGCGATGTGAGACGCTTGGAAATGCCAGAATTTAGTGAAGCTGAGGTGCATGAGCTTATCTTCGACA
TTATGGATGATGCACAGCGAAGTGAGTTTGAATCTAATCTAGAGTTAGACTTTTCAATTGAATTGGGTGGTGTGGGTCGA
TTTAGGGTCAACGCCTTTAATCAAAGCCGAGGTGCCTCTGCGGTGTTTCGAACCATCCCCACAACGATTCCAACTCTTGA
AGAGCTTGATTGTCCGCCAATATTTGAAAAAATAGCTAATTACGAGAAAGGGCTAGTTTTAGTGACTGGACCTACCGGTT
CGGGTAAATCAACCACCTTAGCTGCCATGGTTGACTACATTAATCGCCGTCAGAATAAACATATCTTAACCATTGAAGAT
CCTATTGAGTTTGTCCATCAAAGCTCAAAATGCCTGATCAATCAAAGGGAAGTGCATCGCGATACACATAGCTTTAAAGC
AGCTCTTCGTTCAGCATTACGTGAAGACCCGGATGTGATTCTGGTAGGCGAGCTTCGTGACCAAGAAACCATAAGTCTTG
CCCTTACCGCAGCTGAAACAGGACACTTGGTTTTTGGCACTTTGCACACAAGCTCTGCGGCCAAAACCATAGATCGTATT
ATTGATGTATTCCCCGCAGGGGATAAAAATATGGTTCGCTCTATGCTGTCAGAATCATTACGTGCCGTTATCGCGCAAAA
ACTTCTAAAGCGTATCGGTGGAGGGCGAGTAGCATGTCATGAGATCATGCTGGCGAACCCTGCTATTCGTAATCTTGTTC
GGGAAGATAAGGTAGCGCAGATGTACTCAATTATCCAAACGGGTGCCGCTCAAGGCATGAGAACAATGGAGCAGTCGGTA
AATCAACTGATGCAACAGAATCTCGTTAGCAGAGAAGAAGCTGAGGCCAAAGTAGAAATACAGACTGGCTTAGGTACAGG
CTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4Y3HZ80

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

81.176

97.701

0.793
  pilT Vibrio cholerae strain A1552

81.176

97.701

0.793
  pilT Pseudomonas stutzeri DSM 10701

69.322

97.414

0.675
  pilT Pseudomonas aeruginosa PAK

68.824

97.701

0.672
  pilT Acinetobacter baylyi ADP1

69.345

96.552

0.67
  pilT Acinetobacter baumannii D1279779

69.461

95.977

0.667
  pilT Acinetobacter baumannii strain A118

69.461

95.977

0.667
  pilT Acinetobacter nosocomialis M2

69.461

95.977

0.667
  pilT Legionella pneumophila strain Lp02

67.066

95.977

0.644
  pilT Legionella pneumophila strain ERS1305867

67.066

95.977

0.644
  pilT Neisseria meningitidis 8013

65.882

97.701

0.644
  pilT Neisseria gonorrhoeae MS11

65.588

97.701

0.641
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.642

96.264

0.497
  pilU Pseudomonas stutzeri DSM 10701

41.791

96.264

0.402
  pilU Vibrio cholerae strain A1552

41.493

96.264

0.399
  pilU Acinetobacter baylyi ADP1

38.393

96.552

0.371

Multiple sequence alignment