Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OCU48_RS01930 Genome accession   NZ_AP024878
Coordinates   401932..403035 (+) Length   367 a.a.
NCBI ID   WP_141346876.1    Uniprot ID   A0A4Y3HZ64
Organism   Vibrio inusitatus strain LMG 23434     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 396932..408035
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCU48_RS01900 - 397585..398190 (-) 606 WP_141346882.1 XTP/dITP diphosphatase -
  OCU48_RS01905 - 398195..398638 (-) 444 WP_141346881.1 DUF4426 domain-containing protein -
  OCU48_RS01910 - 398680..399249 (-) 570 WP_141346880.1 YggT family protein -
  OCU48_RS01915 proC 399268..400092 (-) 825 WP_141346879.1 pyrroline-5-carboxylate reductase -
  OCU48_RS01920 - 400121..400831 (-) 711 WP_141346878.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCU48_RS01925 pilT 400856..401902 (+) 1047 WP_141346877.1 type IV pilus twitching motility protein PilT Machinery gene
  OCU48_RS01930 pilU 401932..403035 (+) 1104 WP_141346876.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCU48_RS01935 ruvX 403039..403464 (-) 426 WP_141346875.1 Holliday junction resolvase RuvX -
  OCU48_RS01940 - 403461..404021 (-) 561 WP_141346874.1 YqgE/AlgH family protein -
  OCU48_RS01945 gshB 404089..405036 (-) 948 WP_141346873.1 glutathione synthase -
  OCU48_RS01950 rsmE 405046..405777 (-) 732 WP_141346872.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OCU48_RS01955 - 405872..406567 (-) 696 WP_141346871.1 endonuclease -
  OCU48_RS01960 - 406700..407194 (-) 495 WP_141346870.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 367 a.a.        Molecular weight: 40872.66 Da        Isoelectric Point: 5.8781

>NTDB_id=89008 OCU48_RS01930 WP_141346876.1 401932..403035(+) (pilU) [Vibrio inusitatus strain LMG 23434]
MELKELLDAMTEHKASDLYITVDSPCMFRVDGELQPFGEKLHQSGVTAILDSIMDNDRRKEYRAIREANFAIESESGRFR
VSAFFQRDLPGAVIRHIETNIPSFDELGLPDTLKELAMVKRGLVLVVGATGSGKSTTLAAMVGHRNQHQRGHILTVEDPI
EFVHQHQKSIVTQREVGLDTESYEIALKNSLRQAPDMIVIGEIRTPETMRYAMTFAETGHLCVATLHANNANQALERILH
LVPQGQREHFLLDLSLNLKGIVGQQLLKAKDDTGRHAALEILLVTPRASDLVRQGEIAEIKNYMTKTKQVGICTFDQSLF
DLVIEGKVTEEEALRSADSANDLRLMLKNHQGTSSSGPLDDIKIDMG

Nucleotide


Download         Length: 1104 bp        

>NTDB_id=89008 OCU48_RS01930 WP_141346876.1 401932..403035(+) (pilU) [Vibrio inusitatus strain LMG 23434]
ATGGAATTAAAAGAACTGTTAGATGCGATGACAGAGCATAAGGCATCGGATCTCTATATCACCGTAGATTCTCCTTGTAT
GTTTCGAGTAGATGGCGAGCTTCAGCCTTTTGGGGAAAAGCTACATCAGTCAGGGGTGACCGCTATCCTTGATAGCATTA
TGGATAACGATAGGCGCAAAGAATATCGAGCCATTAGGGAGGCTAACTTTGCCATAGAAAGTGAATCTGGGCGCTTTAGG
GTCAGTGCTTTCTTTCAGCGAGATCTGCCCGGTGCTGTTATTCGTCATATAGAAACCAATATTCCGTCTTTTGATGAGCT
TGGCTTGCCTGATACGCTGAAAGAGTTGGCGATGGTAAAGCGTGGTTTAGTGCTGGTGGTTGGAGCGACAGGATCAGGAA
AATCCACGACATTGGCAGCGATGGTCGGACATAGAAATCAGCATCAGCGAGGGCATATTCTTACGGTAGAAGACCCTATC
GAGTTTGTGCATCAGCACCAAAAGAGCATAGTCACTCAGCGTGAGGTAGGGTTAGATACAGAAAGTTATGAAATTGCTCT
CAAGAATTCACTAAGACAAGCCCCTGATATGATTGTAATTGGTGAGATTCGAACACCAGAAACTATGCGCTATGCGATGA
CTTTTGCCGAAACAGGTCATCTGTGCGTCGCAACTTTACATGCCAATAATGCTAATCAAGCACTTGAACGAATATTACAC
TTAGTTCCTCAGGGTCAGCGAGAACATTTTTTATTAGATCTATCGCTTAATTTGAAAGGGATCGTGGGTCAGCAATTACT
TAAAGCAAAAGATGATACAGGGCGCCATGCTGCACTAGAGATCTTGCTAGTAACACCAAGAGCTTCGGATCTTGTTCGCC
AAGGAGAGATCGCTGAGATCAAGAACTACATGACAAAAACCAAGCAAGTAGGGATCTGCACCTTTGATCAGTCTCTATTT
GACTTGGTAATAGAAGGTAAGGTCACTGAAGAAGAAGCGTTGCGAAGCGCAGATTCCGCAAACGATCTGCGCTTAATGCT
TAAAAATCATCAAGGAACGAGCAGTAGTGGTCCGTTAGATGATATTAAAATTGATATGGGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4Y3HZ64

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

68.12

100

0.681
  pilU Pseudomonas stutzeri DSM 10701

55.143

95.368

0.526
  pilU Acinetobacter baylyi ADP1

53.022

99.183

0.526
  pilT Legionella pneumophila strain ERS1305867

44.248

92.371

0.409
  pilT Legionella pneumophila strain Lp02

44.248

92.371

0.409
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.13

100

0.392
  pilT Neisseria gonorrhoeae MS11

40.29

94.005

0.379
  pilT Neisseria meningitidis 8013

40.29

94.005

0.379
  pilT Acinetobacter baylyi ADP1

40.469

92.916

0.376
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.018

91.008

0.373
  pilT Vibrio cholerae strain A1552

41.018

91.008

0.373
  pilT Acinetobacter baumannii D1279779

40.118

92.371

0.371
  pilT Acinetobacter baumannii strain A118

40.118

92.371

0.371
  pilT Acinetobacter nosocomialis M2

40.118

92.371

0.371
  pilT Pseudomonas aeruginosa PAK

40.118

92.371

0.371

Multiple sequence alignment