Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RWT08_RS04965 Genome accession   NZ_CP136247
Coordinates   1052804..1053841 (+) Length   345 a.a.
NCBI ID   WP_004147099.1    Uniprot ID   A0AAP7J2J0
Organism   Stenotrophomonas maltophilia strain STEN00241     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1047804..1058841
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RWT08_RS04940 (RWT08_04940) soxR 1049140..1049574 (+) 435 WP_107230054.1 redox-sensitive transcriptional activator SoxR -
  RWT08_RS04945 (RWT08_04945) - 1049617..1050219 (-) 603 WP_249844834.1 short chain dehydrogenase -
  RWT08_RS04950 (RWT08_04950) - 1050317..1051192 (+) 876 WP_249844833.1 LysR family transcriptional regulator -
  RWT08_RS04955 (RWT08_04955) proC 1051199..1052020 (-) 822 WP_249844832.1 pyrroline-5-carboxylate reductase -
  RWT08_RS04960 (RWT08_04960) - 1052039..1052716 (-) 678 WP_249844831.1 YggS family pyridoxal phosphate-dependent enzyme -
  RWT08_RS04965 (RWT08_04965) pilT 1052804..1053841 (+) 1038 WP_004147099.1 type IV pilus twitching motility protein PilT Machinery gene
  RWT08_RS04970 (RWT08_04970) pilU 1053920..1055083 (+) 1164 WP_107230059.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RWT08_RS04975 (RWT08_04975) - 1055170..1055847 (+) 678 WP_249844830.1 hypothetical protein -
  RWT08_RS04980 (RWT08_04980) - 1055959..1056942 (+) 984 WP_249844829.1 aldo/keto reductase -
  RWT08_RS04985 (RWT08_04985) - 1057001..1057897 (-) 897 WP_249844828.1 LysR family transcriptional regulator -
  RWT08_RS04990 (RWT08_04990) - 1058240..1058452 (+) 213 WP_249844827.1 DUF4287 domain-containing protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38658.41 Da        Isoelectric Point: 6.6654

>NTDB_id=889785 RWT08_RS04965 WP_004147099.1 1052804..1053841(+) (pilT) [Stenotrophomonas maltophilia strain STEN00241]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLACPPLFREVIQQPQGLILVTGPTGSGKSTTLAAMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQQYGMMTLDQHL
QDLVKRSLITRNQAREYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=889785 RWT08_RS04965 WP_004147099.1 1052804..1053841(+) (pilT) [Stenotrophomonas maltophilia strain STEN00241]
ATGGATATCGCCGAACTGCTGGCGTTTTCCGTAAAGAACAAAGCGTCCGACCTGCACCTGTCCGCAGGCCTGCCGCCGAT
GATCCGCGTGGACGGCGACGTGCGCCGGATCAACATTCCAGCCCTGGACCACAAGCAGGTCCATGCGCTGGTGTACGACA
TCATGTCCGACAAGCAGCGCCGCGATTACGAAGAATTCCTCGAAGTGGATTTCTCGTTCGAGATCCCGTCGCTGGCGCGC
TTCCGAGTGAACGCGTTCAACCAGAACCGTGGTGCCGGTGCGGTGTTCCGTACCATTCCCTCCGAGGTGCTCACGCTTGA
GGACCTGGCCTGCCCGCCGCTGTTCCGCGAGGTGATCCAGCAGCCGCAGGGCCTGATCCTGGTGACCGGTCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACTACATCAACAAGAACGAATACGGCCACATCCTCACCGTCGAGGAT
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCATCGCGATACGCACGGCTTCAACGA
AGCGTTGCGTTCGGCGCTGCGCGAAGACCCGGACATCATCCTGGTCGGCGAACTGCGTGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGCCACCTGGTGTTTGGCACCCTGCACACCAGTTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAGAAGCCGATGGTGCGCTCGATGCTGTCCGAATCGCTGCGCGCGGTGATCTCGCAGGC
GCTGCTGAAGAAGGTCGGCGGCGGTCGTACCGCAGCGTGGGAAATCATGGTGGGCACCCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGGCCATCCAGACCGGCCAGCAGTACGGCATGATGACCCTGGACCAGCACCTG
CAGGACCTGGTCAAGCGCAGCCTGATCACCCGCAATCAGGCCCGCGAGTACGCCAAGGACAAGCGCCTGTTCGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.651

99.71

0.794
  pilT Acinetobacter baumannii D1279779

79.651

99.71

0.794
  pilT Acinetobacter baumannii strain A118

79.651

99.71

0.794
  pilT Pseudomonas stutzeri DSM 10701

78.488

99.71

0.783
  pilT Acinetobacter baylyi ADP1

77.907

99.71

0.777
  pilT Pseudomonas aeruginosa PAK

79.056

98.261

0.777
  pilT Legionella pneumophila strain Lp02

75

99.71

0.748
  pilT Legionella pneumophila strain ERS1305867

75

99.71

0.748
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.821

95.362

0.675
  pilT Vibrio cholerae strain A1552

70.821

95.362

0.675
  pilT Neisseria meningitidis 8013

65.689

98.841

0.649
  pilT Neisseria gonorrhoeae MS11

65.396

98.841

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

100

0.496
  pilU Vibrio cholerae strain A1552

39.943

100

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.413

98.261

0.397
  pilU Acinetobacter baylyi ADP1

39.706

98.551

0.391