Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RWT08_RS04970 Genome accession   NZ_CP136247
Coordinates   1053920..1055083 (+) Length   387 a.a.
NCBI ID   WP_107230059.1    Uniprot ID   -
Organism   Stenotrophomonas maltophilia strain STEN00241     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1048920..1060083
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RWT08_RS04940 (RWT08_04940) soxR 1049140..1049574 (+) 435 WP_107230054.1 redox-sensitive transcriptional activator SoxR -
  RWT08_RS04945 (RWT08_04945) - 1049617..1050219 (-) 603 WP_249844834.1 short chain dehydrogenase -
  RWT08_RS04950 (RWT08_04950) - 1050317..1051192 (+) 876 WP_249844833.1 LysR family transcriptional regulator -
  RWT08_RS04955 (RWT08_04955) proC 1051199..1052020 (-) 822 WP_249844832.1 pyrroline-5-carboxylate reductase -
  RWT08_RS04960 (RWT08_04960) - 1052039..1052716 (-) 678 WP_249844831.1 YggS family pyridoxal phosphate-dependent enzyme -
  RWT08_RS04965 (RWT08_04965) pilT 1052804..1053841 (+) 1038 WP_004147099.1 type IV pilus twitching motility protein PilT Machinery gene
  RWT08_RS04970 (RWT08_04970) pilU 1053920..1055083 (+) 1164 WP_107230059.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RWT08_RS04975 (RWT08_04975) - 1055170..1055847 (+) 678 WP_249844830.1 hypothetical protein -
  RWT08_RS04980 (RWT08_04980) - 1055959..1056942 (+) 984 WP_249844829.1 aldo/keto reductase -
  RWT08_RS04985 (RWT08_04985) - 1057001..1057897 (-) 897 WP_249844828.1 LysR family transcriptional regulator -
  RWT08_RS04990 (RWT08_04990) - 1058240..1058452 (+) 213 WP_249844827.1 DUF4287 domain-containing protein -
  RWT08_RS04995 (RWT08_04995) - 1058564..1059271 (+) 708 WP_029379885.1 YitT family protein -
  RWT08_RS05000 (RWT08_05000) - 1059306..1059851 (-) 546 WP_249844826.1 DNA-3-methyladenine glycosylase I -

Sequence

Protein


Download         Length: 387 a.a.        Molecular weight: 43195.64 Da        Isoelectric Point: 6.7226

>NTDB_id=889786 RWT08_RS04970 WP_107230059.1 1053920..1055083(+) (pilU) [Stenotrophomonas maltophilia strain STEN00241]
MPHWEPAVNTTATTIDFTSFLKLMAHQRASDLFITAGMPPAMKVNGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKT
HECNFAIGLSGVGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGY
RNHNSTGHIITIEDPIEFVHKHEGCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLC
TLHANNANQAMDRIINFFPEDRRNQLLMDLSLNLKGVVAQQLVPSPDGRSRKVAMEILLGTPLVQDYIRDGEIHKLKEVM
KDSVQLGMKTFDQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEISEIR

Nucleotide


Download         Length: 1164 bp        

>NTDB_id=889786 RWT08_RS04970 WP_107230059.1 1053920..1055083(+) (pilU) [Stenotrophomonas maltophilia strain STEN00241]
ATGCCCCATTGGGAGCCCGCCGTGAACACCACCGCGACCACCATCGATTTCACTTCGTTCCTCAAGCTGATGGCGCACCA
GCGCGCCTCGGACCTGTTCATCACCGCCGGCATGCCGCCGGCGATGAAGGTCAACGGCAAGATCTCGCCGATCACGCAGA
CGCCGCTGACGCCGCAGCAGAGCCGCGACCTGGTCCTCAATGTGATGACCCCGGCGCAGCGCGAGGAGTTCGAGAAGACC
CACGAGTGCAACTTCGCCATCGGCCTGTCCGGTGTCGGCCGCTTCCGTGTCAGCTGCTTCTACCAGCGCAACCAGGTCGG
CATGGTGCTGCGTCGCATCGAGACGCGCATCCCGACCGTGGAAGAGCTGAGCCTGCCGCCGATCATCAAGACGCTGGCGA
TGACCAAGCGCGGCATCATCCTGTTCGTCGGTGCTACCGGTACCGGCAAGTCGACGTCGCTGGCGGCGATGATCGGCTAT
CGCAACCATAATTCGACCGGCCATATCATCACCATCGAAGACCCGATCGAATTCGTGCACAAGCACGAAGGCTGCATCAT
CACCCAGCGCGAAGTGGGCATTGATACCGACAGCTGGGAAGCGGCGTTGAAGAACACCCTGCGCCAGGCGCCGGACGTGA
TCATGATCGGCGAGGTGCGCACCCGCGAGGGCATGGACCATGCCATCGCCTTCGCCGAAACCGGCCATCTGGTGCTGTGC
ACGCTGCATGCCAACAACGCCAACCAGGCGATGGACCGCATCATCAACTTCTTCCCGGAAGACCGCCGCAACCAGCTGCT
GATGGATCTTTCGCTGAACCTCAAGGGTGTGGTGGCGCAGCAGCTGGTGCCTTCGCCCGATGGTCGTTCGCGCAAGGTGG
CGATGGAGATCCTGCTGGGCACGCCGCTGGTGCAGGATTACATCCGCGATGGCGAGATCCACAAGCTGAAGGAAGTGATG
AAGGATTCGGTCCAGCTGGGCATGAAGACCTTCGACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGA
GGACGCATTGCGCTACGCCGATTCGCAGAACGAGGTACGCCTGCGCATCAAGCTCAGCCAGGGCGGCGACGCGCGCACCC
TGTCGCAGGGGCTGGACGGCGTGGAGATTTCCGAGATCCGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(129-283)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.12

94.832

0.646
  pilU Acinetobacter baylyi ADP1

63.585

92.248

0.587
  pilU Vibrio cholerae strain A1552

53.09

91.99

0.488
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.817

91.731

0.393