Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OCU28_RS09755 Genome accession   NZ_AP024871
Coordinates   2101510..2102550 (-) Length   346 a.a.
NCBI ID   WP_261816010.1    Uniprot ID   -
Organism   Vibrio gallicus strain CIP 107863     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2096510..2107550
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCU28_RS09725 - 2096801..2097508 (+) 708 WP_261816004.1 endonuclease -
  OCU28_RS09730 rsmE 2097592..2098323 (+) 732 WP_261816005.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OCU28_RS09735 gshB 2098333..2099280 (+) 948 WP_261816006.1 glutathione synthase -
  OCU28_RS09740 - 2099368..2099928 (+) 561 WP_261816007.1 YqgE/AlgH family protein -
  OCU28_RS09745 ruvX 2099925..2100353 (+) 429 WP_261816008.1 Holliday junction resolvase RuvX -
  OCU28_RS09750 pilU 2100350..2101453 (-) 1104 WP_261816009.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCU28_RS09755 pilT 2101510..2102550 (-) 1041 WP_261816010.1 type IV pilus twitching motility protein PilT Machinery gene
  OCU28_RS09760 - 2102575..2103270 (+) 696 WP_261816011.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCU28_RS09765 - 2103331..2105472 (-) 2142 WP_261816012.1 TRAP transporter fused permease subunit -
  OCU28_RS09770 - 2105605..2106558 (-) 954 WP_261816013.1 TAXI family TRAP transporter solute-binding subunit -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38214.75 Da        Isoelectric Point: 6.0494

>NTDB_id=88965 OCU28_RS09755 WP_261816010.1 2101510..2102550(-) (pilT) [Vibrio gallicus strain CIP 107863]
MDITELLRFSVKHNASDLHLSSGLPPMIRIDGDVRRLEIDALSETEVHNLVFDIMDDAQRSEFEAKLELDFSIELDSVGR
FRVNAFQQSRGASAVFRTIPMTIPSLEQLDAPAIFEQIANYERGLVLVTGPTGSGKSTTLAAMVDHINRNQNKHILTIED
PIEFVHHSDKCLINQREVHRDTHSFKAALRSALREDPDVILVGELRDMETISLALTAAETGHLVFGTLHTSSAAKTIDRV
IDVFPAGDKNMVRSMISESLKAVIAQKLLKRVGGGRVACHEVMMANPAIRNLIREDKVAQMYSVIQTGAAHGMKTMEQSA
REMLQKGLVSVDEVNAKIEQTSGIGL

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=88965 OCU28_RS09755 WP_261816010.1 2101510..2102550(-) (pilT) [Vibrio gallicus strain CIP 107863]
ATGGATATCACTGAATTACTTCGCTTTAGTGTAAAGCATAATGCGTCAGATCTACATCTATCATCTGGGTTACCGCCTAT
GATTCGTATAGATGGTGATGTGCGTCGCTTAGAAATCGATGCTTTAAGTGAAACCGAGGTACACAATCTGGTGTTTGACA
TCATGGATGATGCCCAGCGTAGTGAATTTGAGGCAAAGTTAGAGCTGGATTTTTCCATTGAGTTGGATTCGGTGGGACGG
TTTAGGGTTAATGCCTTTCAGCAAAGTAGAGGGGCTTCAGCGGTATTTAGAACCATACCTATGACTATTCCTTCTCTAGA
GCAGTTGGATGCACCTGCAATCTTTGAACAGATTGCAAATTATGAAAGAGGTTTGGTGCTGGTTACAGGCCCAACCGGCT
CTGGGAAATCGACCACTCTTGCGGCTATGGTGGACCATATCAACCGCAATCAAAATAAGCATATATTGACCATTGAAGAC
CCAATTGAATTTGTTCACCACAGCGATAAGTGTTTGATCAATCAAAGAGAGGTACATAGAGATACCCATAGCTTTAAAGC
GGCCTTGAGGTCTGCATTGCGTGAAGACCCCGATGTCATTTTGGTGGGTGAACTGCGAGATATGGAAACCATTAGCTTAG
CGCTAACGGCAGCCGAAACCGGGCATCTGGTGTTTGGTACGTTGCATACCAGTTCAGCGGCAAAGACTATCGATAGGGTT
ATCGATGTATTTCCGGCAGGAGATAAGAATATGGTGCGCTCGATGATCTCTGAATCATTGAAGGCGGTGATTGCGCAGAA
ACTGCTAAAGCGTGTTGGTGGAGGGCGAGTGGCATGCCATGAGGTCATGATGGCAAACCCAGCCATACGTAACCTGATCC
GTGAGGATAAGGTTGCACAGATGTATTCAGTAATTCAAACCGGTGCAGCGCATGGTATGAAGACCATGGAGCAGTCAGCT
CGTGAGATGCTACAAAAGGGTTTGGTCAGCGTTGATGAGGTGAATGCTAAAATAGAGCAAACCTCAGGTATCGGTCTATA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

79.351

97.977

0.777
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

79.351

97.977

0.777
  pilT Pseudomonas stutzeri DSM 10701

68.824

98.266

0.676
  pilT Acinetobacter baumannii strain A118

70.909

95.376

0.676
  pilT Acinetobacter nosocomialis M2

70.909

95.376

0.676
  pilT Acinetobacter baumannii D1279779

70.909

95.376

0.676
  pilT Acinetobacter baylyi ADP1

68.75

97.11

0.668
  pilT Pseudomonas aeruginosa PAK

67.062

97.399

0.653
  pilT Legionella pneumophila strain Lp02

66.667

95.376

0.636
  pilT Legionella pneumophila strain ERS1305867

66.667

95.376

0.636
  pilT Neisseria meningitidis 8013

65.861

95.665

0.63
  pilT Neisseria gonorrhoeae MS11

65.559

95.665

0.627
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.198

96.532

0.494
  pilU Vibrio cholerae strain A1552

40.816

99.133

0.405
  pilU Pseudomonas stutzeri DSM 10701

39.82

96.532

0.384
  pilU Acinetobacter baylyi ADP1

40.187

92.775

0.373

Multiple sequence alignment