Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OCU28_RS09750 Genome accession   NZ_AP024871
Coordinates   2100350..2101453 (-) Length   367 a.a.
NCBI ID   WP_261816009.1    Uniprot ID   -
Organism   Vibrio gallicus strain CIP 107863     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2095350..2106453
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCU28_RS09720 - 2096240..2096734 (+) 495 WP_261816003.1 SprT family zinc-dependent metalloprotease -
  OCU28_RS09725 - 2096801..2097508 (+) 708 WP_261816004.1 endonuclease -
  OCU28_RS09730 rsmE 2097592..2098323 (+) 732 WP_261816005.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OCU28_RS09735 gshB 2098333..2099280 (+) 948 WP_261816006.1 glutathione synthase -
  OCU28_RS09740 - 2099368..2099928 (+) 561 WP_261816007.1 YqgE/AlgH family protein -
  OCU28_RS09745 ruvX 2099925..2100353 (+) 429 WP_261816008.1 Holliday junction resolvase RuvX -
  OCU28_RS09750 pilU 2100350..2101453 (-) 1104 WP_261816009.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCU28_RS09755 pilT 2101510..2102550 (-) 1041 WP_261816010.1 type IV pilus twitching motility protein PilT Machinery gene
  OCU28_RS09760 - 2102575..2103270 (+) 696 WP_261816011.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCU28_RS09765 - 2103331..2105472 (-) 2142 WP_261816012.1 TRAP transporter fused permease subunit -

Sequence

Protein


Download         Length: 367 a.a.        Molecular weight: 40826.53 Da        Isoelectric Point: 5.9868

>NTDB_id=88964 OCU28_RS09750 WP_261816009.1 2100350..2101453(-) (pilU) [Vibrio gallicus strain CIP 107863]
MELSQILEAMIEHKASDLYITVDSPCMFRVDGELIAFGECLHQSGVNALLDSIMDNDRRKEYRNIRESNFAIESGEGRFR
VSAFYQRDLPGAVIRHIETVIPSMEQLGLPDSIKQLALTKRGLVLVVGATGSGKSTTLAAMVGHKNNQHKGHILTVEDPI
EFVHQHHSCIVTQREVGLDTESYEIALKNSLRQAPDMIVIGEIRTAETMRYAMTFAETGHLCVATLHANNANQALERILH
LVPKEQREHFLLDLSLNLKGVVAQQLMRDKQGQGRHLALEVLLATPRVSELIRQGEISEVKSYMSKTLSDGVCTFDRSLF
DLVIEGKVSEEDALRSADSSNELRLKLKNHRGGDSTSSLDDISIDMG

Nucleotide


Download         Length: 1104 bp        

>NTDB_id=88964 OCU28_RS09750 WP_261816009.1 2100350..2101453(-) (pilU) [Vibrio gallicus strain CIP 107863]
ATGGAACTGAGTCAAATATTAGAGGCAATGATCGAGCATAAGGCTTCCGATCTTTATATTACGGTTGATTCACCTTGTAT
GTTTCGAGTTGATGGAGAGTTAATAGCATTTGGGGAGTGCTTACATCAATCTGGAGTGAACGCTTTACTTGATAGCATAA
TGGACAATGATCGCCGCAAAGAATATCGCAATATTAGAGAGTCTAATTTTGCTATTGAGTCCGGTGAAGGGCGATTTAGG
GTAAGTGCTTTTTATCAGCGAGATCTGCCTGGAGCTGTGATACGCCATATTGAGACTGTCATTCCATCCATGGAACAGCT
TGGCTTGCCAGATAGCATTAAGCAGCTGGCATTGACAAAAAGAGGCCTAGTGCTGGTGGTGGGAGCGACCGGTTCTGGTA
AATCAACCACCCTTGCAGCTATGGTTGGACACAAAAACAATCAGCATAAGGGACATATTCTAACCGTTGAAGATCCAATA
GAGTTTGTGCATCAGCATCACTCATGCATTGTTACTCAAAGAGAGGTTGGCTTAGATACAGAAAGCTATGAAATTGCGCT
GAAAAATTCTCTGCGCCAAGCACCAGATATGATAGTGATTGGTGAGATAAGAACTGCGGAAACTATGCGCTATGCAATGA
CCTTTGCTGAGACGGGGCATCTGTGTGTTGCAACCTTGCATGCTAATAATGCCAACCAAGCCTTAGAGCGTATTTTGCAT
CTAGTACCTAAAGAGCAAAGAGAGCACTTTCTACTGGACTTGTCACTAAACCTAAAGGGAGTCGTAGCCCAGCAATTAAT
GCGCGACAAACAAGGCCAAGGACGTCATCTAGCCTTAGAGGTATTGCTTGCTACTCCACGGGTTTCAGAGCTGATTCGAC
AAGGTGAAATCAGCGAAGTGAAGAGCTATATGTCAAAGACACTTAGTGATGGCGTTTGTACCTTTGACCGCTCGCTATTT
GATTTAGTTATAGAGGGGAAGGTGAGTGAAGAAGATGCTCTGAGAAGTGCTGATTCTAGTAATGAATTACGCCTAAAACT
AAAGAATCACCGAGGCGGTGATTCTACAAGCAGCTTAGATGATATCAGCATTGATATGGGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

67.847

100

0.678
  pilU Pseudomonas stutzeri DSM 10701

54

95.368

0.515
  pilU Acinetobacter baylyi ADP1

52.991

95.64

0.507
  pilT Legionella pneumophila strain Lp02

43.824

92.643

0.406
  pilT Legionella pneumophila strain ERS1305867

43.824

92.643

0.406
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.379

94.823

0.392
  pilT Vibrio cholerae strain A1552

41.379

94.823

0.392
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.519

88.283

0.384
  pilT Pseudomonas aeruginosa PAK

41.298

92.371

0.381
  pilT Acinetobacter baumannii D1279779

40.413

92.371

0.373
  pilT Acinetobacter baumannii strain A118

40.413

92.371

0.373
  pilT Acinetobacter nosocomialis M2

40.413

92.371

0.373
  pilT Acinetobacter baylyi ADP1

39.883

92.916

0.371
  pilT Pseudomonas stutzeri DSM 10701

40.118

92.371

0.371
  pilT Neisseria gonorrhoeae MS11

39.017

94.278

0.368
  pilT Neisseria meningitidis 8013

39.017

94.278

0.368

Multiple sequence alignment