Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RW078_RS14290 Genome accession   NZ_CP136178
Coordinates   3035410..3036447 (+) Length   345 a.a.
NCBI ID   WP_006581387.1    Uniprot ID   A0A0A7XL61
Organism   Acinetobacter baumannii strain ABO21-A045     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3030410..3041447
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RW078_RS14280 (RW078_14280) - 3030793..3034389 (+) 3597 WP_317858783.1 AAA family ATPase -
  RW078_RS14285 (RW078_14285) - 3034591..3035283 (-) 693 WP_199989547.1 YggS family pyridoxal phosphate-dependent enzyme -
  RW078_RS14290 (RW078_14290) pilT 3035410..3036447 (+) 1038 WP_006581387.1 type IV pilus twitching motility protein PilT Machinery gene
  RW078_RS14295 (RW078_14295) pilU 3036475..3037593 (+) 1119 WP_000347034.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RW078_RS14300 (RW078_14300) fur 3037662..3038099 (-) 438 WP_001122847.1 ferric iron uptake transcriptional regulator -
  RW078_RS14305 (RW078_14305) - 3038211..3038609 (+) 399 WP_001170994.1 outer membrane protein assembly factor BamE -
  RW078_RS14310 (RW078_14310) - 3038664..3038987 (-) 324 WP_000442585.1 RnfH family protein -
  RW078_RS14315 (RW078_14315) - 3038987..3040054 (-) 1068 WP_002084046.1 hypothetical protein -
  RW078_RS14320 (RW078_14320) - 3040207..3040662 (-) 456 WP_000782976.1 bacteriohemerythrin -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38328.92 Da        Isoelectric Point: 6.7271

>NTDB_id=889425 RW078_RS14290 WP_006581387.1 3035410..3036447(+) (pilT) [Acinetobacter baumannii strain ABO21-A045]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKDVHRLVYDIMNDKQRRDYEEKLETDFSFEVPNVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQIFKDICDYPRGIVLVTGPTGSGKSTTLAAMLDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANHGMTTLDQSL
KGLVSRGVISPQTARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=889425 RW078_RS14290 WP_006581387.1 3035410..3036447(+) (pilT) [Acinetobacter baumannii strain ABO21-A045]
ATGGATATTACAGAGCTACTCGCCTTCTCTGTGAAAAATGGCGCGTCCGATTTACATCTCTCTGCTGGCATGCCACCAAT
GATTCGTGTTGACGGTGAAGTTCGTCGTATTAACTTACCAGCCTTAGAACATAAAGATGTCCACCGTCTTGTTTACGACA
TCATGAATGATAAACAGCGCCGTGACTATGAAGAAAAACTCGAAACTGACTTTTCTTTTGAAGTACCTAATGTTGCGCGT
TTCCGTGTTAACGCATTTAACCAAAACCGTGGTGCTGGTGCAGTATTTCGTACCATTCCATCTAAAGTCCTCACCATGGA
AGATCTAGGTCTAGGACAAATCTTTAAAGATATTTGCGACTATCCTCGCGGCATTGTACTGGTGACGGGGCCAACAGGTT
CGGGTAAATCAACTACGCTTGCAGCGATGCTCGATTATATTAACGAAAACCGCTATGACCATATTTTAACGGTGGAAGAC
CCAATCGAATTTGTACACCAGTCTAAGAAATGTCTCATTAACCAGCGTGAAGTGCATCGTGACACACACGGCTTTAATGA
AGCACTTCGCTCAGCACTGCGTGAAGACCCAGATATTATTTTGGTCGGTGAGATGCGTGACCTTGAAACTATTCGCTTGG
CATTAACCGCTGCCGAAACAGGTCACTTGGTGTTTGGTACACTCCACACCACGTCGGCAGCAAAAACCATTGACCGTGTG
ATTGACGTATTCCCTGCCGAAGAAAAAGACATGGTTCGTGCCATGCTGTCTGAATCTTTACAGGCAGTTATTTCACAGAC
CCTACTTAAAAAGAACGGTGGTGGTCGTGTTGCAGCGCATGAAATTATGATTGGTATTCCGGCTATTCGTAACTTAATTC
GTGAAAACAAAGTCGCACAAATGTATTCAGCTATTCAGACTGGTGCTAACCATGGCATGACAACCCTCGACCAAAGCCTA
AAAGGTTTGGTTTCACGTGGTGTAATTAGTCCACAAACAGCCCGTACAGCGGCGAAACAGCCTGAATCATTTCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0A7XL61

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

100

100

1
  pilT Acinetobacter baumannii D1279779

99.71

100

0.997
  pilT Acinetobacter baumannii strain A118

99.71

100

0.997
  pilT Acinetobacter baylyi ADP1

88.663

99.71

0.884
  pilT Pseudomonas aeruginosa PAK

84.012

99.71

0.838
  pilT Pseudomonas stutzeri DSM 10701

83.721

99.71

0.835
  pilT Legionella pneumophila strain Lp02

75.581

99.71

0.754
  pilT Legionella pneumophila strain ERS1305867

75.581

99.71

0.754
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.429

97.391

0.696
  pilT Vibrio cholerae strain A1552

71.429

97.391

0.696
  pilT Neisseria meningitidis 8013

68.116

100

0.681
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.678
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.522
  pilU Vibrio cholerae strain A1552

41.593

98.261

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.101

0.394
  pilU Acinetobacter baylyi ADP1

38.728

100

0.388