Detailed information NTG0889426

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: RW078_RS14295
• Genome accession: NZ_CP136178
• Coordinates: 3036475..3037593 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347034.1
• Uniprot ID: A0A009HMD8
• Organism: Acinetobacter baumannii strain ABO21-A045
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3031475..3042593

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RW078_RS14285 (RW078_14285)	-	3034591..3035283 (-)	693	WP_199989547.1	YggS family pyridoxal phosphate-dependent enzyme	-
RW078_RS14290 (RW078_14290)	pilT	3035410..3036447 (+)	1038	WP_006581387.1	type IV pilus twitching motility protein PilT	Machinery gene
RW078_RS14295 (RW078_14295)	pilU	3036475..3037593 (+)	1119	WP_000347034.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
RW078_RS14300 (RW078_14300)	fur	3037662..3038099 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
RW078_RS14305 (RW078_14305)	-	3038211..3038609 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
RW078_RS14310 (RW078_14310)	-	3038664..3038987 (-)	324	WP_000442585.1	RnfH family protein	-
RW078_RS14315 (RW078_14315)	-	3038987..3040054 (-)	1068	WP_002084046.1	hypothetical protein	-
RW078_RS14320 (RW078_14320)	-	3040207..3040662 (-)	456	WP_000782976.1	bacteriohemerythrin	-
RW078_RS14325 (RW078_14325)	-	3040915..3041763 (-)	849	WP_000336551.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41891.09 Da
• Isoelectric Point: 6.6943

>NTDB_id=889426 RW078_RS14295 WP_000347034.1 3036475..3037593(+) (pilU) [Acinetobacter baumannii strain ABO21-A045]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=889426 RW078_RS14295 WP_000347034.1 3036475..3037593(+) (pilU) [Acinetobacter baumannii strain ABO21-A045]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCCGATCTTTTTATTACAGATGGTGTTGCACCATC
GATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGCCTTTCAGGTGAAGTAATTGGTCAACTCTTACATTCCA
TCATGAGCGAAAAACAACGCAAAGAATTTGCAGACACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCAGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCGCTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGAAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (34-270)

3D structure

• AlphaFold DB: A0A009HMD8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	69.022	98.925	0.683
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.908	93.011	0.39