Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OCU46_RS01935 Genome accession   NZ_AP024866
Coordinates   398991..400037 (+) Length   348 a.a.
NCBI ID   WP_141273163.1    Uniprot ID   A0A4Y3IS66
Organism   Vibrio comitans strain LMG 23416     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 393991..405037
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCU46_RS01905 hemW 394556..395737 (-) 1182 WP_141273151.1 radical SAM family heme chaperone HemW -
  OCU46_RS01910 - 395734..396339 (-) 606 WP_141273153.1 XTP/dITP diphosphatase -
  OCU46_RS01915 - 396344..396787 (-) 444 WP_141273155.1 DUF4426 domain-containing protein -
  OCU46_RS01920 - 396815..397384 (-) 570 WP_141273157.1 YggT family protein -
  OCU46_RS01925 proC 397403..398227 (-) 825 WP_141273159.1 pyrroline-5-carboxylate reductase -
  OCU46_RS01930 - 398256..398966 (-) 711 WP_141273161.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCU46_RS01935 pilT 398991..400037 (+) 1047 WP_141273163.1 type IV pilus twitching motility protein PilT Machinery gene
  OCU46_RS01940 pilU 400067..401170 (+) 1104 WP_141273165.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCU46_RS01945 ruvX 401174..401599 (-) 426 WP_141273167.1 Holliday junction resolvase RuvX -
  OCU46_RS01950 - 401596..402156 (-) 561 WP_141273169.1 YqgE/AlgH family protein -
  OCU46_RS01955 gshB 402224..403171 (-) 948 WP_141273171.1 glutathione synthase -
  OCU46_RS01960 rsmE 403181..403912 (-) 732 WP_141273173.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OCU46_RS01965 - 404007..404663 (-) 657 WP_167495437.1 endonuclease -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38585.06 Da        Isoelectric Point: 5.9305

>NTDB_id=88916 OCU46_RS01935 WP_141273163.1 398991..400037(+) (pilT) [Vibrio comitans strain LMG 23416]
MDITELLRFSVKHNASDLHLSSGLPPMVRIDGDVRRLEMPEFSEAEVHDLIFDIMDDAQRSEFESNLELDFSIELGGVGR
FRVNAFNQSRGASAVFRTIPTTIPTLEQLDCPPIFEKIANYEKGLVLVTGPTGSGKSTTLAAMVDYINRHHNKHILTIED
PIEFVHQSSKCLINQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGDKNMVRSMLSESLRAVIAQKLLKRIGGGRVACHEIMLANPAIRNLVREDKVAQMYSIIQTGAAQGMRTMEQSV
NQLMQQNLVSREEAEAKVEIQTGLGTGF

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=88916 OCU46_RS01935 WP_141273163.1 398991..400037(+) (pilT) [Vibrio comitans strain LMG 23416]
ATGGATATCACCGAATTGCTTCGCTTTAGTGTAAAGCATAATGCCTCAGATCTACATCTTTCATCGGGACTACCGCCAAT
GGTAAGGATTGATGGCGATGTGAGACGCTTGGAAATGCCTGAATTTAGTGAAGCTGAGGTACACGATCTTATCTTCGACA
TTATGGATGATGCGCAGCGAAGTGAGTTTGAATCAAATCTAGAGTTAGATTTCTCAATTGAACTGGGTGGCGTTGGCCGT
TTTAGGGTTAATGCCTTCAACCAAAGTCGTGGCGCTTCTGCGGTGTTCCGAACCATACCCACAACCATTCCAACTCTTGA
GCAACTTGATTGCCCTCCAATATTTGAAAAAATAGCCAATTATGAGAAGGGGCTAGTCTTAGTCACTGGACCTACGGGGT
CGGGTAAATCAACCACCCTAGCAGCCATGGTTGACTACATTAATCGTCATCACAACAAGCATATCTTAACCATTGAAGAT
CCTATTGAGTTTGTCCATCAAAGCTCAAAGTGTCTGATTAACCAAAGGGAAGTGCATCGTGATACACATAGCTTTAAAGC
CGCACTTCGCTCAGCATTACGTGAAGACCCTGATGTGATTTTGGTGGGGGAGCTTCGTGACCAAGAAACCATAAGCCTTG
CCCTAACCGCAGCAGAAACGGGGCATTTAGTTTTTGGCACTCTACACACGAGTTCTGCAGCAAAAACCATAGACCGTATT
ATTGATGTATTTCCTGCGGGTGATAAAAACATGGTCCGTTCTATGTTGTCGGAATCACTGCGCGCCGTTATCGCACAAAA
ACTTCTAAAGCGTATTGGAGGAGGGCGAGTGGCATGTCATGAGATCATGTTAGCGAACCCAGCTATTCGAAACCTTGTTC
GGGAAGATAAGGTGGCGCAGATGTACTCAATTATCCAAACTGGCGCGGCTCAAGGCATGAGAACGATGGAGCAGTCGGTA
AATCAACTGATGCAACAAAACCTAGTTAGCCGAGAAGAAGCTGAGGCCAAAGTAGAAATACAAACTGGCTTAGGTACAGG
CTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4Y3IS66

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

82.059

97.701

0.802
  pilT Vibrio cholerae strain A1552

82.059

97.701

0.802
  pilT Pseudomonas stutzeri DSM 10701

69.322

97.414

0.675
  pilT Acinetobacter baylyi ADP1

69.345

96.552

0.67
  pilT Pseudomonas aeruginosa PAK

68.529

97.701

0.67
  pilT Acinetobacter baumannii D1279779

69.461

95.977

0.667
  pilT Acinetobacter baumannii strain A118

69.461

95.977

0.667
  pilT Acinetobacter nosocomialis M2

69.461

95.977

0.667
  pilT Legionella pneumophila strain Lp02

67.066

95.977

0.644
  pilT Legionella pneumophila strain ERS1305867

67.066

95.977

0.644
  pilT Neisseria meningitidis 8013

65.294

97.701

0.638
  pilT Neisseria gonorrhoeae MS11

65

97.701

0.635
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.343

96.264

0.494
  pilU Pseudomonas stutzeri DSM 10701

41.493

96.264

0.399
  pilU Vibrio cholerae strain A1552

41.493

96.264

0.399
  pilU Acinetobacter baylyi ADP1

38.69

96.552

0.374

Multiple sequence alignment